Heavy metals, antibiotics and nutrients affect the bacterial community and resistance genes in chicken manure composting and fertilized soil

Deng, Wenwen; Zhang, Anyun; Chen, Shujuan; He, Xueping; Jin, Lan; Yu, Xiumei; Yang, Sheng; Li, Bei; Fan, Liangqian; Lin, Junjie; Pan, Xin; Zou, Likou

doi:10.1016/j.jenvman.2019.109980

Cited by 124 publications

(36 citation statements)

References 80 publications

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“…In a case study carried out in Northern Ireland by Zhao et al (2019b) , there were positive correlations between 24 types of metals and ARGs, particularly for that of Cr, Zn, and β-lactam ARGs. Regarding the influence of heavy metals, antibiotics, and nutrients on ARGs in fertilized soil, Deng et al (2019) demonstrated the notable dependence of ARG formation on copper elements. Likewise, similar conclusions were also be drawn by Knapp et al (2011) who reported the selection pressure induced by Cu, Cr, Ni, Pb, and Fe to the content of ARGs in Scottish soil.…”

Section: Discussionmentioning

confidence: 99%

The Interactions Between Antibiotic Resistance Genes and Heavy Metal Pollution Under Co-Selective Pressure Influenced the Bio-Enzyme Activity

Zheng

et al. 2021

Front. Chem.

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The spread of antibiotic resistance genes (ARGs) has brought potential risks to public health. However, the interactions between heavy metals and ARGs, as well as their potential effect on bio-enzyme activity under the pressure of co-selectivity in soil still remain poorly understood. In this work, the distribution characteristics and the co-selective relationship of 28 ARGs and eight heavy metals in soil in a dairy farm were visualized via the geographic information system (GIS) technique. Eight kinds of heavy metals were detected by an atomic fluorescence spectrometer and atomic absorption spectrophotometer, which were further evaluated via the single factor pollution index value. The GIS analysis showed that arsenic (As) was the key element responsible for soil pollution, which was found to be positively related to soil depths. The top three comprehensive scores of ARGs ranked the orders of sul2 > tetX > blaTEM, indicating the high potential of risk caused by these genes in the soil environment. In addition, the functional predications performed with the 16 SrDNA sequencing data based on the KEGG database indicated that the sulfonamides in soil involved multiple pathways, especially the metabolism, transport and catabolism, and membrane transport processes. This suggested that most bio-enzymes were found to be expressed in low activities in different pathways. Significant correlations were observed between the heavy metals and ARGs (p < 0.05), particularly between the ARGs and As, Cu, Ni, Pb, and Zn (p < 0.01). This study offers deep insights into the potential interactions between heavy metals and ARGs in soil and provides guidance for the fabrication of enzyme-based smart materials for soil remediation in dairy farms.

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Section: Discussionmentioning

confidence: 99%

The Interactions Between Antibiotic Resistance Genes and Heavy Metal Pollution Under Co-Selective Pressure Influenced the Bio-Enzyme Activity

Zheng

et al. 2021

Front. Chem.

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“…We speculated that manure affect soil ARGs from three ways: firstly, nutrients carried by manure cause the proliferation of endogenous bacteria carrying by ARGs; secondly, ARGs from manure transfer to soil endogenous microorganisms; and finally, manure-derived ARG survival in soil. Nutrients can enhance the effect of selective pressures of ARGs (Deng et al, 2020); even sterilized manure also can result in a little increase in the relative abundance of tetracyclineresistant bacteria in soil (Peng et al, 2016). Therefore, the seven significantly enriched ARGs in three manure treatments (Figure 3) may result from the proliferation of endogenous resistance using nutrients carried by the manures.…”

Section: Small Proportion Of Manure-derived Bacteria and Antibiotic Resistance Gene Survival In Soil May Be The Main Reason For Its Minormentioning

confidence: 99%

“…Long-term fertilization with chemical fertilizers plus mushroom residues showed a more connective and closer bacterial networks compared with chemical fertilization (Yao et al, 2020). Recent studies about ARGs mainly shed light on the dynamics of ARGs during manure treatment process (Gou et al, 2018;Awasthi et al, 2019;Wu et al, 2020) and in agricultural soils following application of animal manures (Deng et al, 2020;Liu et al, 2021). Little attention was paid to ARGs in plant-derived organic manure, which also contain a huge number of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Chicken Manure and Mushroom Residues Affect Soil Bacterial Community Structure but Not the Bacterial Resistome When Applied at the Same Rate of Nitrogen for 3 Years

et al. 2021

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Animal manure is a reservoir of antibiotic resistance genes (ARGs), and direct application of the manure will lead to spread of ARGs in farmland. Here, we explored the impacts of chicken manure and heat-treated chicken manure on the patterns of soil resistome after 3 years’ application, with mushroom residues set as the plant-derived organic manure treatment. A total of 262 ARG subtypes were detected in chicken manure using high-throughput qPCR, and heat treatment can effectively remove 50 types of ARGs. Although ARG subtypes and abundance were both higher in chicken manure, there was no significant difference in the ARG profiles and total ARG abundance among three manure-treated soils. Soil bacteria community compositions were significantly different among manure-treated soils, but they were not significantly correlated with soil ARG profiles. Fast expectation–maximization microbial source tracking (FEAST) was used for quantifying the contributions of the potential sources to microbial taxa and ARGs in manure-fertilized soil. Results revealed that only 0.2% of the chicken manure-derived bacterial communities survived in soil, and intrinsic ARGs were the largest contributor of soil ARGs (95.8–99.7%); ARGs from chicken manure only contributed 0.4%. The total ARG abundance in the heat-treated chicken manure-amended soils was similar to that in the mushroom residue-treated soils, while it was 1.41 times higher in chicken manure-treated soils. Thus, heat treatment of chicken manure may efficiently reduce ARGs introduced into soil and decrease the risk of dissemination of ARGs.

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“…Salmonella disease in calves prompts fever and looseness of the bowels, with high mortality if no anti-toxin treatment is given (Birhanu et al 2018). S. typhimurium contamination in day-old chicks additionally prompts enteric and other fundamental illnesses, with a high death rate (Nascimento et al 2019), while disease in more seasoned chicks prompts asymptomatic colonization of dung with relentless discharge of living organisms in defecation (Deng et al 2020). S. enterica subspecies enteric serotype typhimurium (S. typhimurium) is perceived as a human microorganism and represents a sanitation hazard around the world.…”

Section: Species Of Zoonotic Importancementioning

confidence: 99%

Pathology and Public Health Significance of Salmonella

2021

Int J Vet Sci

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Heavy metals, antibiotics and nutrients affect the bacterial community and resistance genes in chicken manure composting and fertilized soil

Cited by 124 publications

References 80 publications

The Interactions Between Antibiotic Resistance Genes and Heavy Metal Pollution Under Co-Selective Pressure Influenced the Bio-Enzyme Activity

The Interactions Between Antibiotic Resistance Genes and Heavy Metal Pollution Under Co-Selective Pressure Influenced the Bio-Enzyme Activity

Chicken Manure and Mushroom Residues Affect Soil Bacterial Community Structure but Not the Bacterial Resistome When Applied at the Same Rate of Nitrogen for 3 Years

Pathology and Public Health Significance of Salmonella

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