Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil

Aransiola, Elizabeth Funmilayo; Ige, OO; Ehinmitola, E O; Layokun, S. K.

doi:10.5897/ajb2016.15823

Cited by 15 publications

(7 citation statements)

References 18 publications

(20 reference statements)

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“…This strain is usually isolated from hydrocarbon-contaminated areas and tolerates high concentrations of heavy metals and hydrocarbons. Moreover, it is also an important part of the microbial community that is involved in petroleum biodegradation processes (Aransiola et al 2017;Rodrigues et al 2009). In this manner, the clinical and environmental Kp1 and Kp2 strains were investigated in terms of their petroleum degradation abilities.…”

Section: Resultsmentioning

confidence: 99%

“…Klebsiella pneumoniae, a gram-negative, coccobacilli, facultative, non-motile, usually encapsulated bacteria belonging to Enterobacteriaceae, is an important opportunistic pathogen besides its ability to live in different environments (Aransiola et al 2017;Lee et al 2013;Rajkumari et al 2018). Its ability to survive in different habitats and to degrade the organic compounds such as petroleum are related to its genetic structure.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the petroleum removal efficiencies and plasmid profiles of clinical and environmental Klebsiella pneumoniae strains

Özyürek

Avcıoğlu

2021

Int. J. Environ. Sci. Technol.

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It is of great importance to remove petroleum and petroleum-based products from the environment through the genes encoded by the chromosomal and plasmids of microorganisms. This study aimed to compare the petroleum removal efficiencies and plasmid profiles of clinical Klebsiella pneumoniae ATCC 700721 (Kp1) and environmental Klebsiella pneumoniae ATCC 13883 (Kp2) strains. Over 80% of petroleum removal and high bacterial growths were achieved by Kp1 and Kp2 strains. The degradation rate constants and half-life periods of Kp1 and Kp2 strains were 0.262 and 0.280; 2.64 and 2.47 days, respectively. Moreover, the culture supernatants of Kp1 and Kp2 strains reached to 87.5% and 81% in degradation of petroleum. The Gas Chromatography-Mass Spectrometry analysis confirmed that Kp1 and Kp2 strains degraded approximately 90% and 80% of long chain n-alkanes. Kp1 and Kp2 exhibit > 12kbp plasmid DNA band. Plasmid curing was applied with ethidium bromide to determine the role of plasmid and genomic DNA in petroleum degradation. Kp1 and Kp2 strains (plasmid cured) showed over 50% of degradation abilities even in the absence of plasmid DNA. So, the results clearly indicated that both genomic and plasmid DNA of Kp1 and Kp2 contributed to petroleum degradation process. Consequently, the clinical Kp1 and environmental Kp2 strains are promising for further studies as they can be effective in petroleum bioremediation even under unsuitable environmental conditions. Editorial Responsibility: Samareh Mirkia.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the petroleum removal efficiencies and plasmid profiles of clinical and environmental Klebsiella pneumoniae strains

Özyürek

Avcıoğlu

2021

Int. J. Environ. Sci. Technol.

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“…Klebsiealla planticola grew anaerobically at a Cd dose of 15 mM and precipitated CdS (Sharma et al, 2000). Aransiola et al (2017) reported that K. edwardsii, K. oxytoca, and K. pneumoniae strains isolated from diesel polluted soil significantly reduced Cadmium, copper and nickel. Several studies have revealed that mechanism of bio-sorption were convincingly associated to physicochemical interaction of metal in solution (Aksu et al, 2002;Lodeiro et al, 2006;Amini et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Klebsiealla planticola grew anaerobically at a Cd dose of 15 mM and precipitated CdS ( Sharma et al., 2000 ). Aransiola et al (2017) reported that K. edwardsii, K . oxytoca, and K. pneumoniae strains isolated from diesel polluted soil significantly reduced Cadmium, copper and nickel.…”

Section: Discussionmentioning

confidence: 99%

Halotolerant and metalotolerant bacteria strains with heavy metals biorestoration possibilities isolated from Uburu Salt Lake, Southeastern, Nigeria

Orji

Awoke

Aja

et al. 2021

Heliyon

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Environmental contaminations by heavy metals are currently an increasing public health concern globally. One key challenge of these toxic metals is the extremely difficulties involved in their detoxification from the environment and effluents because of their non-degradability. An efficient biologic agent with potentials of remediating these toxic metals may ease these ever-increasing problems. We reported toxic metals tolerance and bioremediation potentials of novel bacteria sp. Strains USL2S, USL4W and USL5W isolated from Uburu salt lake, Ebonyi State, Nigeria. The phenotypic characteristics and the 16S rRNA gene analyses revealed that USL2S strain belongs to the genus Klebsiella, whereas USL4W and USL5W strains belong to the genus Pseudomonas. The bacteria isolates grew well in media containing 5-15 % of sodium chloride. The bacteria isolate showed capacity to tolerate 50.0 mM Hg þ2 and Pb þ2 , 17.0, 12.50 and 4.0mM Ni þ2 , Cd þ2 , and Zn þ2 respectively in solid media. Pseudomonas putida A4W Strain also tolerated 16.0 mM Cu þ2 , while Klebsiella sp. Strain USL2S, Pseudomonas putida USL5W Strain tolerated 4.0 mM each.

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“…Klebsiella sp. yang diisolasi dari tanah yang tercemar bahan bakar terbukti resisten terhadap kromium, kadmium, dan tembaga (Aransiola et al, 2017). Acinetobacter sp.…”

Section: Hasil Dan Pembahasan Uji Resistensi Acinetobacter Sp Irc2 Terhadap Berbagai Logam Beratunclassified

Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

Irawati

Hasthosaputro

Kusumawati³

2020

J. Biol Papua

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The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS). It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore, it is also resistant to heavy metal mixture of 4.5 mM. The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively. In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics. Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri

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Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil

Cited by 15 publications

References 18 publications

Comparison of the petroleum removal efficiencies and plasmid profiles of clinical and environmental Klebsiella pneumoniae strains

Comparison of the petroleum removal efficiencies and plasmid profiles of clinical and environmental Klebsiella pneumoniae strains

Halotolerant and metalotolerant bacteria strains with heavy metals biorestoration possibilities isolated from Uburu Salt Lake, Southeastern, Nigeria

Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

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