Heavy oils produced by Aureobasidium pullulans

Manitchotpisit, Pennapa; Price, Neil P. J.; Leathers, Timothy D.; Punnapayak, Hunsa

doi:10.1007/s10529-011-0548-1

Cited by 56 publications

(40 citation statements)

References 12 publications

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“…It is well-reported for various applications, such as production of pullulans, extracellular polysaccharide, siderophore, single-cell protein, and hydrolytic enzymes including amylases, proteases, esterases, pectinases, xylanases, and mannanases (Chi et al 2009;Ravella et al 2010;Rumbold et al 2003). Besides that, Manitchotpisit et al (2011) discovered 21 potential strains of A. pullulans which can produce extracellular heavy oil of related structures. Generally, the fungus is regarded as safe for biotechnological and even environmental applications.…”

Section: Introductionmentioning

confidence: 99%

Emergence of Aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis

et al. 2011

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Despite the great importance of Aureobasidium pullulans in biotechnology, the fungus had emerged as an opportunistic human pathogen, especially among immunocompromised patients. Clinical detection of this rare human fungal pathogen presently relies on morphology diagnosis which may be misleading. Thus, a sensitive and accurate quantitative molecular assay for A. pullulans remains lacking. In this study, we presented the microscopy observations of A. pullulans that reveals the phenotypic plasticity of the fungus. A. pullulans-specific primers and molecular beacon probes were designed based on the fungal 18S ribosomal RNA (rRNA) gene. Comparison of two probes with varied quencher chemistry, namely BHQ-1 and Tamra, revealed high amplification efficiency of 104% and 108%, respectively. The optimized quantitative real-time PCR (qPCR) assays could detect and quantify up to 1 pg concentration of A. pullulans DNA. Both assays displayed satisfactory performance parameters at fast thermal cycling mode. The molecular assay has great potential as a molecular diagnosis tool for early detection of fungal infection caused by A. pullulans, which merits future study in clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Emergence of Aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis

et al. 2011

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“…The oils inhibited small cell lung cancer cultures to a similar degree, while the oil from strain CU 43 more strongly inhibited an oral cavity cancer cell line. The results indicate that the heavy oils from different strains have different effects on specific mammalian cell types (Manitchotpisit et al, 2011).…”

Section: Heavy Oils From a Pullulans And Their Biosynthesismentioning

confidence: 96%

“…As stated below, some strains of A. pullulans can produce heavy oils (Manitchotpisit et al, 2011) and some others can produce PMA in the presence of CaCO 3 (Zhang et al, 2011). Even some strains of A. pullulans can produce the antibiotics aureobasidin A against some fungal strains (Slightom et al, 2009) and siderophore which can inhibit growth of some Vibrio spp.…”

Section: #2mentioning

confidence: 99%

Taxonomy ofAureobasidiumspp. and biosynthesis and regulation of their extracellular polymers

Chi

Wang

et al. 2013

Critical Reviews in Microbiology

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The genus Aureobasidium spp. have been divided into three species, A. pullulans. A. leucospermi and A. proteae, and A. pullulans has been known to have five varieties. However, after analysis of many strains of this yeast isolated from different environments, they do not belong to any of the three species or the five varieties. Although pullulan produced by A. pullulans has been widely used in different fields in industry and different strains of this yeast has been known to produce poly(β-L-malic acid) (PMA), heavy oils and β-1,3-glucan, it is still unknown how the black yeast synthesizes and secretes the extracellular polymers at molecular level. In this review article, new biosynthetic pathways of pullulan, PMA and heavy oils, the enzymes and their genes related to their biosynthesis and regulation are proposed. Furthermore, some enzymes and their genes related to pullulan biosynthesis in A. pullulans have been characterized. But it is completely unknown how pullulan is secreted and how PMA, heavy oils and β-1,3-glucan are synthesized and secreted. Therefore, there is much work to be done about taxonomy and biosynthesis, secretion and regulation of pullulan, PMA, heavy oils and β-1,3-glucan at molecular levels in Aureobasidium spp.

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“…Precipitated PMA was dissolved in 200 mL of Milli-Q water (Millipore, Billerica, MA) and lyophilized. Heavy oil was extracted from the cell pellets using methyl ethyl ketone as described by Manitchotpisit et al [14]. The experiments were performed in triplicate and standard errors are reported.…”

Section: Pma Production By Strains Of a Pullulansmentioning

confidence: 99%

“…We recently completed a multilocus molecular phylogeny of A. pullulans [13]. Interestingly, certain phylogenetically deWned clades produced high levels of speciWc bioproducts, including pullulan, xylanase, and heavy oil [13,14]. The objective of this study is to examine PMA production by genetically diverse phylogenetic clades of A. pullulans, in an eVort to identity groups that produce high levels of PMA.…”

Section: Introductionmentioning

confidence: 99%

Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans

Manitchotpisit

Skory

Peterson

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Poly(β-L-malic acid) (PMA) is a natural biopolyester that has pharmaceutical applications and other potential uses. In this study, we examined PMA production by 56 strains of the fungus Aureobasidium pullulans representing genetically diverse phylogenetic clades. Thirty-six strains were isolated from various locations in Iceland and Thailand. All strains from Iceland belonged to a newly recognized clade 13, while strains from Thailand were distributed among 8 other clades, including a novel clade 14. Thirty of these isolates, along with 26 previously described strains, were examined for PMA production in medium containing 5% glucose. Most strains produced at least 4 g PMA/L, and several strains in clades 9, 11, and 13 made 9-11 g PMA/L. Strains also produced both pullulan and heavy oil, but PMA isolated by differential precipitation in ethanol exhibited up to 72% purity with no more than 12% contamination by pullulan. The molecular weight of PMA from A. pullulans ranged from 5.1 to 7.9 kDa. Results indicate that certain genetic groups of A. pullulans are promising for the production of PMA.

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Heavy oils produced by Aureobasidium pullulans

Cited by 56 publications

References 12 publications

Emergence of Aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis

Emergence of Aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis

Taxonomy ofAureobasidiumspp. and biosynthesis and regulation of their extracellular polymers

Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans

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