Hedysari Extract Improves Regeneration after Peripheral Nerve Injury by Enhancing the Amplification Effect

Abstract

Radix Hedysari is an herbal preparation frequently used in traditional Chinese medicine. It can promote regeneration after peripheral nerve injury, but its effect on the amplification ratio (the ratio of distal to proximal fibers) during peripheral nerve regeneration has not yet been examined. In this study, we explored the effect of Hedysari extract on the amplification ratio in the peripheral nerve. Male Sprague-Dawley rats were separated into three groups at random: normal group (without surgery), model group (given sleeve nerve bridging surgery, but without adjuvant treatment) and treatment group (given sleeve nerve bridging surgery and then given Hedysari extract as adjuvant treatment). Twelve weeks after surgery, general observations, electrophysiological examination, histological analysis, morphometric measurements, and amplification ratio calculations were made. The results showed that nerve conduction velocity, the fiber and axon diameter, the g-ratio, the number of regenerating nerve fibers and the amplification ratio were better in the treatment group than in the model group, suggesting that Hedysari extract can effectively promote the growth of lateral buds in the proximal nerve stump and substantially improve the amplification effect during peripheral nerve regeneration.

The advances in nerve tissue engineering: From fabrication of nerve conduit toin vivonerve regeneration assays

“…Many studies have shown that various types of vitamins such as vitamin B complex, vitamin B12 (Altun & Kurutas, ), vitamin E (Elsy, Khan, & Maheshwari, ), vitamin D3 (Chabas et al, ), vitamin D2 (Chabas et al, ), and vitamin C (Gess, Rohr, & Young, ) are effective in nerve regeneration. In addition, joint application of Radix Hedysari plant extract, an herbal preparation often used in traditional Chinese medicine, combined with a chitin conduit could efficiently promote the growth of lateral buds in the proximal nerve stump and considerably improve regeneration in a peripheral nerve injury model (Wang et al, ). Liu et al () have also demonstrated that Salidroside, a derivative of Rhodiola rosea L. plant, can promote nerve regeneration in cellular nerve scaffolds through salidroside‐induced neurotrophin secretion in SCs.…”

Experimental immunological demyelination enhances regeneration in autograft-repaired long peripheral nerve gaps

“…Despite the development of several surgical techniques15, new bridging materials16171819, and therapies2021222324, the autologous nerve graft is still the “gold standard” in long peripheral nerve gap reconstruction. Although there are some disadvantages to the procedure, such as a lack of autologous nerve sources or feeling obstacles for providing area during surgery, the autologous nerve graft is closest to the normal physiological structure and function when the above-noted difficulties can be overcome.…”

Ginsenoside Re Promotes Nerve Regeneration by Facilitating the Proliferation, Differentiation and Migration of Schwann Cells via the ERK- and JNK-Dependent Pathway in Rat Model of Sciatic Nerve Crush Injury

“…Previous studies have manifested that actin cytoskeleton reorganization is a crucial factor in many cellular progress, including cell proliferation (Charvet et al 2006;Papakonstanti and Stournaras 2008;Yue et al 2011), migration, and differentiation (Konakahara et al 2011. It is reported that lots of traditional Chinese medicine can ameliorate SNC (Zou et al 2013) and promote peripheral nerve regeneration (Kou et al 2013;Wang et al 2013b). These all forced us to question whether Re participating in the cytoskeleton reorganization may play a vital role in regulating the proliferation and differentiation of SCs after SNC.…”