Helicobacter pylori — Current Therapy and Future Therapeutic Strategies

Siddalingam, Rajinikanth; Chidambaram, Kumarappan

doi:10.5772/58338

Cited by 6 publications

(7 citation statements)

References 124 publications

(141 reference statements)

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“…Therefore, access for antimicrobial drugs to the infected site is limited by both the acidic gastric lumen affecting drug stability and mucus gel layer which hinders the attainment of sufficient antibiotic concentrations for bactericidal activity (Lopes et al, 2014;Umamaheshwari et al, 2004). Therefore, a new treatment approach that is also simple to encourage patient compliance aiming to achieve higher eradication rates is urgently needed (Gatta et al, 2013;Siddalingam and Chidambaram, 2014;Vakil and Vaira, 2013). On these assumptions, a stomach-site specific drug delivery system would increase the localized concentration and the residence time of the drugs at the site of action making less variable the gastric emptying time, reducing dosage frequency, and increasing patient compliance.…”

Section: Introductionmentioning

confidence: 96%

“…However, this latter condition is relevantly affected by inter-individual differences in gastric emptying as well as by the amount and variety of the ingested food. Even with a right selection of drugs, bacterial eradication may fail in up 20-40% of patients owing to antibiotic resistance, insufficient antibiotic concentration reaching the site of infection (under the gastric mucus gel layer), uneven drug distribution in the gastric lumen, short contact time between drug formulation and gastric mucosa, and poor patient compliance due to the complex therapeutic regimen which also produces side effects (Gasparetto et al, 2012;Siddalingam and Chidambaram, 2014;WGO Global Guidelines, 2010). Among these difficulties connected with Hp cure, failure of therapy is mostly associated to antibiotic resistance and patient non-compliance (Graham and Shiotani, 2008).…”

Section: Introductionmentioning

confidence: 97%

“…from the administration and that the transport of an antibiotic from the gastric lumen through the mucus layer is more effective than drug absorbed through the basolateral membrane from blood circulation as well as drug administered by means a conventional formulation (Prasanthi et al, 2011;Siddalingam and Chidambaram, 2014;Umamaheshwari et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Gastroretentive montmorillonite-tetracycline nanoclay for the treatment of Helicobacter pylori infection

Iannuccelli¹,

Maretti²,

Montorsi³

et al. 2015

International Journal of Pharmaceutics

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Gastroretentive montmorillonite-tetracycline nanoclay for the treatment of Helicobacter pylori infection

Iannuccelli¹,

Maretti²,

Montorsi³

et al. 2015

International Journal of Pharmaceutics

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“…(4) Stool antigen detection using rapid qualitative tests is an efficient method that indicates a current infection, the occurrence of a reinfection and monitoring treatment effectiveness, but it has the disadvantage of low reproducibility; the method is acceptable for adult patients. (2,3) The profile of serum antibodies compared to different antigens of the Helicobacter pylori bacterium can be monitored using the (Immunoblot Test). This is a quantitative, non-invasive method allowing the simultaneous study of antibodies against several antigen fractions.…”

Section: Introductionmentioning

confidence: 99%

Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies in Pediatric Infection with Helicobacter Pylori

Iftimi¹,

Stănescu²,

Anton³

et al. 2020

Rev. Chim.

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The current methods for the diagnosis of Helicobacter pylori infection in children include invasive (direct) methods, that enable the detection of the bacteria or the bacterial urease in gastric biopsies, or noninvasive (indirect) methods, which consist of researching specific antibodies, antigens and urease in other different samples (serum, saliva, urine, stool, exhaled air). The urease functions in H. pylori infection is to neutralize gastric acid by producing NH3 through the hydrolysis of urea. Monochloramine, a NH3-derived compounds has cytotoxic effects on host cells. The Western Blot method refers to the extraction of a a sodium dodecyl sulphate from a Helicobacter pylori strain followed by a separation of the solubilised protein using discontinous polyacrylamide gel electrophoresis according to molecular mass and transfer of the separated proteins to nitrocellulose. The ELISA (enzyme-linked immunosorbent assay) technique refers to the detection of Anti-Helicobacter pylori antibodies type Ig G. We also used an immunochromatographic system with the lateral leakage test strip based on the principle of the sandwich technique with monoclonal antibodies. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold �labelled antibodies and complementary oligonucleotide-labelled immobilized at different places on a nitrocellulose membrane. Our study aims to assess the performances of the Western Blot method for determining anti-Helicobacter pylori Ig A and Ig G antibodies in correlation with clinical data and other available diagnosis methods. For validation purposes, the results were compared to those obtained using the enzyme-linked immunosorbent assay technique, gastric biopsy and immunochromatography.

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“…H. pylori infection is chronic and if acquired remains life long, unless eradicated by antibiotics. [1][2][3]…”

Section: Introductionmentioning

confidence: 99%

Capsule in Capsule Dosage Form With Mini Tablets for the Treatment of Helicobacter Pylori

Bhilegaonkar¹,

Kunde²,

Naik³

2018

Int. Res. J. Pharm.

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The current investigation involves proton pump inhibitor based therapy (Lansoprazole + Clarithromycin + Amoxicillin) combining three drugs with Capsule in capsule technology for the treatment of Helicobacter pylori improving patient convenience and compliance. For delivery of Amoxicillin and Clarithromycin in stomach, combination mini tablets were formulated by direct compression method of the two drugs. Evaluation of the blend flow properties were carried out on powder blends and subsequently compressed to formulate mini tablets and evaluated for physicochemical properties, drug content and in vitro release study. The optimized formulation was subjected to short term stability study at accelerated conditions of temperature and relative humidity of 40ºC ± 2ºC and 75% ± 5% RH and evaluated at required time intervals for physical appearance and drug content. All evaluation results were found to be within the acceptable range. Lansoprazole being acid labile was filled in size 5 capsules and capsules were enteric coated with Eudragit S 100 for intestinal delivery. For final delivery of combination doses, the mini tablets were filled into the 00 size capsules and an enteric coated Lansoprazole capsule was placed in it implementing capsule in capsule technology. These capsules were evaluated as the final dosage forms for the drug content and in vitro release profile. All the tests exhibited results within an acceptable range. Hence capsule in capsule dosage form can be a good alternative for the delivery of three drugs for the treatment of H.pylori infection for better patient compliance.

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Helicobacter pylori — Current Therapy and Future Therapeutic Strategies

Cited by 6 publications

References 124 publications

Gastroretentive montmorillonite-tetracycline nanoclay for the treatment of Helicobacter pylori infection

Gastroretentive montmorillonite-tetracycline nanoclay for the treatment of Helicobacter pylori infection

Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies in Pediatric Infection with Helicobacter Pylori

Capsule in Capsule Dosage Form With Mini Tablets for the Treatment of Helicobacter Pylori

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