Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment

Abstract

Background: Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential. Objectives: This study was conveyed to detect H.pylori in gastric biopsies specimens by using routine Hematoxylin, Modified Giemsa dye as well as immunohistochemical stain, besides to assess the specificity and sensitivity of Helicobacter microbe detection in each method. Patients and methods: The research was both prospective and retrospective, carried out on 100 cases of endoscopically obtained gastric biopsies. Data obtained from archives of the pathology department, at AL-Jamhuri Teaching Hospital, Mosul city, and collected in a period spanning from April 2013 to March 2014. The information included; Age, sex, gastric biopsy location, inflammation status, the presence of dysplasia or carcinoma. Helicobacter pylori infection was assessed histochemically and immunohistochemically. Results: In a total of 100 gastric samples, patients' age range was 11 to 82 years (mean age of 46.5 years), with a male to female ratio of 1.38:1. Helicobacter pylori bacilli were positive with H&E/MGS in 71 (71%) of cases, increased to 75 (75%) case with IHC. Chronic gastritis noticed in 85 biopsy specimens, 74% were positive for H.pylori. There was a statistically significant difference between IHC and H&E/MGS (p=0.04) for detection of H.pylori. The sensibility and specificity of the H&E/MGS were measured compared with the recommended standard sensitive and specific IHC test; they were 95% and 100% respectively. Conclusion: The routine ancillary stains request for the detection of H.pylori remains a laboratory and an institution right. This study revealed that, in our laboratory, the regular application of ancillary dyes is not obliged for the description of H.pylori because it was readily recognizable in the bulk of sections with haematoxylin staining. However, we recommend the use of IHC in specific circumstances.

Molecular Mechanism of Helicobacter pylori-Induced Gastric Cancer

“…The association between Helicobacter pylori (H. pylori) infection and the development of gastric cancer has been well studied [12]. In developing countries, 70 to 90% of people carry H. pylori before the age of 10 years old, but in developed countries, the prevalence of infection varies from 25 to 50% [13]. Helicobacter pylori account for more than 60% of gastric cancers.…”

An Immuno-Histochemical Assessment of Ki67, P53 Over-Expression in Helicobacter Pylori Positive Gastritis.

“…In this study, the age range from 14-84 years with mean 46 years ± 17.2, male to female ratio was 7:8, in Morales-Fuentes et al (28) study the range of age was 55-81 years and male to female ratio 5:6, while in Azza et al (25) study the patients mean age was 53 years ± 5.1 and male to female ratio 1.75:1, and in Zainab et al (29) study the patients' age range was 11 -82 years (mean of 46.5 years) with a male to female ratio of 1.38:1. Ki67 & P53 expressions in this study were signi cantly detected more in age group (42-55) years, also the expression of both Ki67 & P53 were seen in females more than males .This discrepancy of results among different studies might be due to bias in data collection of some researches.…”

Is Molecular Analysis Mandatory for Better Post-surgical Outcome in Sleeve Gastrectomy Specimens with Asymptomatic Helicobacter pylori Colonization? A Study from Pakistan

“…At least two histopathologists independently analyzed the biopsies microscopically regarding the grading of chronic gastritis according to the updated Sydney system. Biopsies were observed for H. pylori density, neutrophil infiltration, degree of chronic inflammation, glandular hyperplasia, glandular atrophy, intestinal metaplasia, dysplasia, surface erosions, lymphoid aggregates, and follicles (9)(10)(11). Dixon visual analog scale was used to grade variables as mild, moderate, and severe (9,10).…”