Helicobacter pylori infection in long-term and short-term Japanese visitors to Peru

Ramírez-Ramos, Alberto; Gilman, Robert H.; Watanabe, José; Recavarren, A Sixto; Spira, William M.; Miyagui, Juan; Rodríguez, Upegui; Ramírez-Icaza, C.

doi:10.1016/s0140-6736(94)91673-x

Cited by 10 publications

(11 citation statements)

References 4 publications

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“…All suitable volunteers provided a stool sample for HpSA and underwent 14 C UBT using the PY test kit and a microCOUNT scintillation counter (Ballard Medical Products, Draper, Utah), conducted at the Department of Pathology, Cayetano Heredia Hospital. Quantification was reported as disintegrations per minute.…”

Section: Methodsmentioning

confidence: 99%

“…At 1 month following completion of treatment, 14 C UBT and HpSA tests were repeated. If the result of this posttreatment UBT was negative, the UBT was repeated 1 week later for confirmation.…”

Section: Methodsmentioning

confidence: 99%

“…From among 273 dyspeptic individuals (18 to 55 years) initially recruited from a shantytown in Lima, Peru, 238 participants who met the inclusion criteria and were suspected to be H. pylori positive based on 14 C urea breath test (UBT) results underwent endoscopy. Participants with endoscopy-proven infections received standard eradication therapy and were monitored by UBT and HpSA at 1 month following treatment and at 3-month intervals for 9 months posttreatment.…”

mentioning

confidence: 99%

“…Consistent with the suggestion that major transmission risk factors are poor sanitation and overcrowding, infection rates of over 70% were found in even younger age groups in lower socioeconomic classes (10). Over 80% of Peruvian patients undergoing endoscopy to investigate symptoms of gastritis are infected with H. pylori (14,16).…”

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confidence: 99%

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Posttreatment Follow-Up of Helicobacter pylori Infection Using a Stool Antigen Immunoassay

Roth

Taylor

Gilman

et al. 2001

Clin Diagn Lab Immunol

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The Helicobacter pylori stool antigen enzyme immunoassay (HpSA) was evaluated during posttreatment follow-up of patients in a country with a very high prevalence of H. pylori infection. From among 273 dyspeptic individuals (18 to 55 years) initially recruited from a shantytown in Lima, Peru, 238 participants who met the inclusion criteria and were suspected to be H. pylori positive based on 14 C urea breath test (UBT) results underwent endoscopy. Participants with endoscopy-proven infections received standard eradication therapy and were monitored by UBT and HpSA at 1 month following treatment and at 3-month intervals for 9 months posttreatment. A second endoscopy was performed if UBT results showed evidence of treatment failure or H. pylori recurrence. Biopsy results were considered the "gold standard" in all analyses. Among patients who underwent endoscopy, HpSA had a pretreatment sensitivity of 93%. Two-hundred thirty patients completed the treatment regimen, of whom 201 (93%) were considered to have had successful treatment outcomes based on a negative follow-up UBT. Thirty-two patients with UBT-defined treatment failures or H. pylori recurrences at any point during the 9-month follow-up underwent a second endoscopy. In the posttreatment setting, HpSA had an overall sensitivity of 73% and a specificity of 67%. Agreement between UBT and HpSA diminished throughout the follow-up. Among 14 participants in whom HpSA remained positive at 1 month following treatment despite UBT evidence of treatment success, 12 (86%) became HpSA negative within 3 months posttreatment. Although this study confirmed the validity of the HpSA in the initial assessment of dyspeptic patients, the test demonstrated a reduced overall accuracy in the detection of treatment failures and H. pylori recurrences during 9 months of posttreatment follow-up. Furthermore, in some patients it may take up to 3 months after successful eradication for antigen shedding to diminish to levels within the negative HpSA range.Helicobacter pylori was first linked with gastritis in 1975 and was subsequently shown to be associated with peptic ulcer in 1985. Since then, the detection of the spiral bacterium in the gastric mucosa has become a principal aspect of the diagnostic investigation of patients with upper gastrointestinal symptoms suggestive of peptic ulcer disease (7). Chronic superficial gastritis due to infection with H. pylori is responsible for up to 95% of duodenal ulcers and 80% of gastric ulcers throughout the world (25). Perhaps the most convincing evidence for the pathogenic role of H. pylori is the dramatic effect of antibiotic therapy. Current treatment regimens combining at least two antibiotics and a proton pump inhibitor can eradicate H. pylori infections in greater than 90% of patients (21), and they significantly reduced ulcer recurrence in long-term follow-up studies (29). The organism has also been classified as a class I carcinogen due to its pathogenic role in intestinal-type gastric adenocarcinoma and has further been linked to diffus...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Posttreatment Follow-Up of Helicobacter pylori Infection Using a Stool Antigen Immunoassay

Roth

Taylor

Gilman

et al. 2001

Clin Diagn Lab Immunol

Self Cite

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“…Poor sanitation and crowding are considered risk factors for H. pylori transmission (13,19,23,27). For example, among Peruvian adults, some 90% of Lima shantytown residents but only about half of more prosperous people are infected with H. pylori (19,20,25).…”

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confidence: 99%

Validation of String Test for Diagnosis of Helicobacter pylori Infections

et al. 2006

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The method of recovering Helicobacter pylori DNA or viable cells absorbed on a string that a person has swallowed and that is retrieved an hour later (string test) should be a useful alternative to traditional analysis of cells or DNA obtained by endoscopy, which is invasive, uncomfortable, relatively costly, and ill-suited for community-based and pediatric studies. Here we assayed the sensitivity and validity of the string test versus conventional endoscopic biopsy for detecting and analyzing H. pylori infection. Forty-four people with gastric complaints were studied using both H. pylori culture and urease gene (ureB) PCR. H. pylori organisms cultured from strings and biopsy specimens from the same patients were fingerprinted by the randomly amplified polymorphic DNA (RAPD) method. Biopsy sections were also hematoxylin and eosin and silver stained for H. pylori detection. H. pylori was cultured from 80% of strings and detected by PCR from 91% of strings from participants whose biopsies had been H. pylori positive by culture, PCR, and/or histology. Strains recovered from strings and biopsy specimens yielded identical or closely related RAPD profiles in each of the 24 cases tested. We conclude that the string test is a useful method for H. pylori recovery and analysis when relatively noninvasive procedures are needed.

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Occurrence of Helicobacter pylori in surface water in the United States

1999

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The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water‐borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively respiring micro‐organisms binding monoclonal anti‐H. pylori antibody were found in the majority of surface and shallow groundwater samples tested (n = 62), indicating that H. pylori may be present in aquatic environments in the US and supporting a water‐borne route of transmission for this organism. There was no significant correlation between the occurrence of either total coliforms or Escherichia coli in the water and the presence of H. pylori. Our results indicate that routine screening of water supplies for the presence of traditional indicator organisms may fail to protect the consumer from exposure to H. pylori.

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Helicobacter pylori infection in long-term and short-term Japanese visitors to Peru

Cited by 10 publications

References 4 publications

Posttreatment Follow-Up of Helicobacter pylori Infection Using a Stool Antigen Immunoassay

Posttreatment Follow-Up of Helicobacter pylori Infection Using a Stool Antigen Immunoassay

Validation of String Test for Diagnosis of Helicobacter pylori Infections

Occurrence of Helicobacter pylori in surface water in the United States

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