Helicobacter Pylori Infection in Patients With Different Gastrointestinal Diseases From Northern Brazil

Abstract

-Background -The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of different diseases, the search for pathogenicity markers such as CagA and VacA alleles by molecular techniques has intensified. Objectives -To investigate the presence of H. pylori infection and the frequency of different genotypes of this bacterium in patients with gastrointestinal diseases from Northern Brazil, and to establish their association with the histopathological findings. Methods -In a prospective study, samples were collected from 554 patients with different gastrointestinal diseases (gastritis, duodenal ulcer, gastric ulcer, and gastric cancer) seen at a referral hospital attending the entire State of Pará, located in the metropolitan region of Belém. Data such as gender and age obtained with an epidemiological questionnaire were analyzed. The presence of H. pylori and the bacterial genotype were investigated by PCR. Gastric biopsies were assessed histologically. Results -The prevalence of H. pylori infection was 91%. Infection was more frequent among patients with gastric ulcer and gastric cancer. In these groups, there was a predominance of men and older patients when compared to the other two groups studied. The predominant bacterial genotype was s1m1cagA+, which was more frequent among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between s1m1cagA+ strains and a higher degree of inflammation, neutrophil activity and development of intestinal metaplasia. Conclusion -The present study demonstrates a high incidence of H. pylori infection in the patients analyzed, especially among those with gastric ulcer and gastric cancer. Virulent s1m-1cagA+ strains predominated and were associated with more severe lesions. HEADINGS -Helicobacter pylori. Helicobacter infections. Gastrointestinal diseases. Genotype.

Gender, Age, Endoscopic Findings, Urease and Helicobacter Pylori: All Uncorrelated Within a Sample of a High Gastric Cancer Prevalence Population in Amazon

“…It is widely assumed that H. pylori can induce chronic active gastritis, both of which, in turn, can lead to the development of gastric cancer. Indeed, a high incidence of H. pylori infection was found recently in a cross-sectional study with 554 patients from Belém, mainly among patients with gastric ulcer and gastric cancer (4) .…”

Time Trends of Helicobacter Pylori Prevalence in Itajaí - Sc: A Retrospective Study of 25 Years Based on Endoscopic Database

“…It presents as main reservoir the human stomach, colonizing its epithelium. Several studies have already demonstrated its relationship with gastrointestinal diseases, and the presence of the bacterium is a risk factor for chronic and atrophic gastritis, peptic ulcer disease, intestinal metaplasia, gastric adenocarcinoma and mucosal lymphoid tissue lymphoma (MALT) (1,2,3) .…”

Prevalence of <i>Helicobacter pylori vacA</i>, <i>cagA</i>, <i>dupA</i> and <i>oipA</i> Genotypes in Patients with Gastric Disease

“…After surviving in the stomach environment, flagellar motility is necessary to move the bacterium through the gastric epithelium cells. The interaction of H. pylori adhesins and cellular receptors supports gastric mucosa infection, and it is followed by the secretion of virulence factors (such as vacA, cagA, dupA and oipA genes) which cause tissue damage [1] [3] [4].…”