Helicobacter pylori lipids can form ordered membrane domains (rafts)

Huang, Zhen; London, Erwin

doi:10.1016/j.bbamem.2019.183050

Cited by 14 publications

(17 citation statements)

References 65 publications

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“…But at the same time, the surface affinity between C. albicans and H. pylori towards hydrophobic surfaces, and between them, is higher. These results are consistent with description of lipid profiles of H. pylori , from which has been evidenced a high relative content of lysophospholipids which have been associated with a better adherence to epithelial cells in vitro [72] , but also, with the fact that H. pylori strains produce several characteristic lipids, e.g., steryl glycosides and cholesteryl glucosides [73] . For the case of C. albicans , its surface hydrophobicity is well-known and has been associated to the promotion of adhesion process on surfaces; however, for C. albicans , the presence of negative charges on its surface also have been documented [61] , [74] .…”

Section: Resultssupporting

confidence: 90%

Multiple surface interaction mechanisms direct the anchoring, co-aggregation and formation of dual-species biofilm between Candida albicans and Helicobacter pylori

Palencia

García

Palencia

2022

Journal of Advanced Research

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Section: Resultssupporting

confidence: 90%

Multiple surface interaction mechanisms direct the anchoring, co-aggregation and formation of dual-species biofilm between Candida albicans and Helicobacter pylori

Palencia

García

Palencia

2022

Journal of Advanced Research

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“…Lipid rafts are small (10−200 nm), heterogeneous, highly dynamic, tightly packed liquid ordered state microdomains in bilayers that are generally enriched in both lipids with predominantly saturated acyl chains and sterol. They are thought to coexist with disordered membrane domains that are rich in lipids with unsaturated acyl chains [37][38][39] . Since the incubation temperature of 67 °C could achieve maximum drug loading, it is concluded that the extent of microdomain formation may be temperature-dependent.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Docetaxel Loading Conditions in Liposomes: proposing potential products for metastatic breast carcinoma chemotherapy

Vakili‐Ghartavol

Rezayat

Faridi‐Majidi

et al. 2020

Sci Rep

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Docetaxel (DtX) was loaded in nanoliposomes based on a new remote loading method using mannitol and acetic acid as hydration buffer. DTX loading conditions were optimized, and the final formulations were prepared according to the best parameters which were HSPC/mPEG2000-DSPE/Chol (F1), HSPC/mPEG2000-DSPE/DPPG/Chol (F2), HSPC/mPEG2000-DSPE/DSPG/Chol (F3), at molar ratios of 85/5/10, 80/5/5/10, 80/5/5/10, respectively. DTX-liposomes were found of desired size (~115 nm) and homogeneity (pDi ≤ 0.2), high drug encapsulation efficacy (34-67%) and DTX concentration, and favorable stability. Passive loaded counterparts liposomes showed three times lower encapsulation efficacy compared to the remote loaded liposomes. The drug release of remote loaded liposomes in plasma 50% was significantly more controlled and less in comparison with their passive loaded counterparts (p < 0.0001). The IC50 values of formulations were determined on MCF-7, 4T1, TUBO, NIH/3T3 cell lines. The biodistribution of iodinated docetaxel as free or liposomal form exhibited significantly greater accumulation of DTX-liposomes in tumors than that of free docetaxel due to the EPR effect. In vivo experiment with BALB/c mice bearing 4T1 or TUBO breast carcinoma tumors also showed that DTX-liposomes could significantly delay tumor growth and prolonged the survival time in comparison with control and Taxotere groups at the similar dose of 8 mg/kg. F1 and F2 formulations were stable and showed good anti-tumor activity and merit further investigation. Taxanes have extremely low solubility in water and pharmaceutical concerns caused by their bulky polycyclic structure 1,2. Nanocarriers, including liposomes, micelles, and polymeric or inorganic nanoparticles have been developed to prepare a higher therapeutic efficacy, lower toxicity, and controlled delivery of chemotherapeutic agents 1,3-8. Among them, liposomes, composed of phospholipids, have been explored for their use in clinically approved formulations for more than five decades 6,9-15. Liposomes, compared with other nanocarriers, present specific advantages and promising capabilities in delivering a plethora of otherwise inefficient drugs by modifying their physicochemical characteristics and biodistribution, and by reducing the toxic effects of drugs 6,16,17. Moreover, chemical and biological stability under different storage conditions of agents and during blood circulation, and biocompatible characteristics recommend them as carriers for therapeutic agents 6,9,17,18. There are currently two liposomal formulations of docetaxel (DTX) in clinical development. LE-DT (NeoPharm, Inc.) and ATI-1123 (Azaya Therapeutics, Inc.) have undergone phase I studies and appears to be well tolerated (ClinicalTrials.gov in May 2019). In general, drug loading into the liposomes is attained by either passive or active (remote) methods 19. In passive loading method, dried thin film is hydrated in aqueous solutions containing the drug of interest. In contrast, remote loading technique is loading of a drug into performed lip...

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“…However, these were indirect measurements. Clearer evidence of domain formation in living systems was obtained by extracting intact cell membranes from living cells( Baumgart et al, 2007 , Sezgin et al, 2012 , Huang et al, 2019 ), which allowed for more detailed studies, but with the caveat that one was looking at extracted membranes and not functional natural membranes in situ . It was revealed that the difficulty in observing raft domains optically could be due to their physical size.…”

Section: Domain Formation and Function In Living Systemsmentioning

confidence: 99%