Helicobacter pylori stool antigen test: A method to confirm eradication in children

Roggero, Patricia; Bonfiglio, A.; Luzzani, S.; Valadè, A.; Cataliotti, E.; Corno, Giuseppe Del; Garlaschi, Maria Gristina; Carissimi, Emanuela; Mosca, Fabio; Carnelli, V.

doi:10.1067/mpd.2002.123662

Cited by 32 publications

(26 citation statements)

References 6 publications

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“…This test has confirmed its value in the past both in adults 21 and in children, [22][23][24][25][26][27] especially for pretreatment diagnosis. The stability of the antigens to be detected has been previously mentioned by the manufacturer and confirmed in a study in which bacteria were experimentally spiked in stools.…”

Section: Discussionsupporting

confidence: 73%

Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Mégraud¹

2005

The Journal of Pediatrics

104

106

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Section: Discussionsupporting

confidence: 73%

Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Mégraud¹

2005

The Journal of Pediatrics

104

106

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“…In the case of infants (34,35,39) most studies have used ROC curve analysis (46) and showed better sensitivity and specificity values. In this study we tried to adjust the cutoff value for patients less than 6 years old, with the aim of improving the accuracy of the test for this age group, however, there was no improvement in sensitivity values without an important decrease in specificity values.…”

Section: Discussionmentioning

confidence: 99%

“…The test was evaluated in children in developed countries and showed a satisfactory accuracy in children and adolescents, however, there is a need for studies in children in developing countries, where the method is not widely established (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40). This study aimed to evaluate the accuracy of the HpSA test for diagnosis of H. pylori infection in children and adolescents.…”

mentioning

confidence: 98%

Evaluation of the Stool Antigen Test for Helicobacter pylori in Children and Adolescents

2005

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The stool antigen test for Helicobacter pylori is a noninvasive immunoassay to diagnose active infection with Helicobacter pylori in human fecal samples. Its performance in children and teenagers has been tested in some developed countries, showing a sensitivity and specificity above 90%, however, its accuracy in developing countries and in children under 6 years is not well established. To determine the accuracy of the test for diagnosing Helicobacter pylori infection in children and teenagers, we evaluated 133 patients (4 months to 17 years old). The gold standard was a positive culture or positive histology and rapid urease test. The test was done according to the manufacturer's instructions. However, modifications were introduced for better reproducibility. Samples were analyzed twice and results are expressed as optical density (OD) determined spectrophotometrically at 450 nm. HpSA was considered positive at OD > or =0.160 and negative at OD <0.140. One hundred twenty-seven of the 133 (95.5%) patients were included. There were no infected infants. The test showed a 94.6% sensitivity (95% CI: 90.6-98.5) and a 96.5% specificity (95% CI: 93.3-99.7). At ages 2 to 6 years the specificity was 96.4% (95% CI: 85.1-99.2) and the sensitivity was 80.0% (95% CI: 64.8-89.7), at ages 6 to 10 years the sensitivity was 100.0% and the specificity 95.7%, and above 10 years the sensitivity and specificity were 100.0%. We conclude that the test is efficient in adolescents and children, however there is a need for further studies with a greater number of patients for evaluation of its accuracy in infants.

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“…Neither keeping the samples at room temperature for up to 5 days nor freezing for months or even years seems to influence the accuracy of the stool tests [63,101,116,126]. In most countries, an EIA would be less costly than the UBT.…”

Section: Antigen Detectionmentioning

confidence: 96%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

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The array of tests that can be used for diagnosis of Helicobacter pylori infection is large, and it can be confusing to define which test to use particularly in children where results may not be comparable to those obtained in adult patients. Using PubMed, we reviewed the English literature from January 1999 to May 2009 to identify articles that determined sensitivity and specificity of H. pylori invasive and non-invasive diagnostic tests in children. We excluded articles that presented a review of the literature, abstracts, case reports, or series where children's results could not be separated from adult populations. Of the tissue based methods, rapid urease tests have better sensitivity than histology to detect presence of H. pylori; however, histology can detect the pathology associated with disease including gastritis, intestinal metaplasia, and other conditions that could be the cause of the child's symptoms. Culture of gastric tissues or stool has 100% specificity but sensitivity is low. Of the serologic tests, immunoblot has the best sensitivity. The urea breath tests have >75% sensitivity for detection of H. pylori before and after treatment. Immunoassays in stool using monoclonal antibodies have >95% sensitivity for detection of H. pylori before and after treatment. PCR testing can be performed in tissue and stool samples and can detect genes associated to antibiotic resistance. In summary, the current commercial non-invasive tests have adequate sensitivity and specificity for detecting the presence of H. pylori; however, endoscopy with histopathology is the only method that can detect H. pylori and lesions associated with the infection.

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Helicobacter pylori stool antigen test: A method to confirm eradication in children

Cited by 32 publications

References 6 publications

Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Evaluation of the Stool Antigen Test for Helicobacter pylori in Children and Adolescents

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

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