HeLp, a Heme Lipoprotein from the Hemolymph of the Cattle Tick,Boophilus microplus

Maya‐Monteiro, Clarissa M.; Daffre, Sirlei; Logullo, Carlos; Lara, Flávio Alves; Alves, Elias W.; Capurro, Margareth Lara; Zingali, Russolina B.; Almeida, Igor C.; Oliveira, Pedro L.

doi:10.1074/jbc.m007344200

Cited by 92 publications

(94 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among higher organisms, the tick Boophilus microplus (Braz et al, 1999) and the hemipteran Rhodnius prolixus , both blood-sucking arthropods, are the only exceptions reported to date. We have recently shown that HeLp (Haem Lipoprotein), the major lipoprotein of Boophilus microplus haemolymph, is capable of binding haem and delivering it to the tick tissues (Maya-Monteiro et al, 2000). The same protein has also been described in two other tick species and therefore its presence may be a common feature of ticks (Gudderra et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Lara

Lins

Paiva-Silva

et al. 2003

Journal of Experimental Biology

Self Cite

107

View full text Add to dashboard Cite

The hard tick Boophilus microplus ingests large volumes of cattle blood, as much as 100 times its own mass before feeding. Huge amounts of haem are produced during haemoglobin digestion, which takes place inside acidic lysosomal-type vacuoles of the digest cells of the midgut. Haem is a promoter of free radical formation, so haemoglobin digestion poses an intense oxidative challenge to this animal.In the present study we followed the fate of the haem derived from haemoglobin hydrolysis in the digest cells of the midgut of fully engorged tick females. The tick does not synthesize haem, so during the initial phase of blood digestion, absorption is the major route taken by the haem, which is transferred from the digest cells to the tick haemocoel. After this absorptive period of a few days, most of the haem produced upon haemoglobin degradation is accumulated in the interior of a specialized, membrane-delimited, organelle of the digest cell, herein called hemosome. Haem accounts for 90% of the hemosome mass and is concentrated in the core of this structure, appearing as a compact, non-crystalline aggregate of iron protoporphyrin IX without covalent modifications. The unusual FTIR spectrum of this aggregate suggests that lateral propionate chains are involved in the association of haem molecules with other components of the hemosome, which it is proposed is a major haem detoxification mechanism in this bloodsucking arthropod.

show abstract

Section: Discussionmentioning

confidence: 99%

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Lara

Lins

Paiva-Silva

et al. 2003

Journal of Experimental Biology

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…In this regard, HeLp, a heme transporting protein in the hemolymph of R. microplus, has been shown to bind heme species with different Soret bandwidth compared to other proteins that bind heme, suggesting multiple quantum states for bound heme (Maya-Monteiro et al 2000). The hemosome, a special organelle in ticks where excess heme is stored for detoxification purposes, also has a supramolecular structure that differs from that of hemozoin (Lara et al 2003).…”

Section: Heme the Centre Of Lifementioning

confidence: 99%

Ancestral reconstruction of tick lineages

Mans

Castro

Pienaar

et al. 2016

Ticks and Tick-borne Diseases

101

View full text Add to dashboard Cite

“…A heme-binding lipoprotein, HeLp, has been described in this tick as a special adaptation. HeLp transports heme in the hemolymph and peripheral tissues, primarily the ovaries (Maya-Monteiro et al, 2000). The ferritin gene from different species of ticks (Kopáček et al, 2003;Xu et al, 2004) has already been identified.…”

Section: Introductionmentioning

confidence: 99%

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Galay

Aung

Umemiya‐Shirafuji

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYTicks are obligate hematophagous parasites and important vectors of diseases. The large amount of blood they consume contains great quantities of iron, an essential but also toxic element. The function of ferritin, an iron storage protein, and iron metabolism in ticks need to be further elucidated. Here, we investigated the function a newly identified secreted ferritin from the hard tick Haemaphysalis longicornis (HlFER2), together with the previously identified intracellular ferritin (HlFER1). Recombinant ferritins, expressed in Escherichia coli, were used for anti-serum preparation and were also assayed for iron-binding activity. RT-PCR and western blot analyses of different organs and developmental stages of the tick during blood feeding were performed. The localization of ferritins in different organs was demonstrated through an indirect immunofluorescent antibody test. RNA interference (RNAi) was performed to evaluate the importance of ferritin in blood feeding and reproduction of ticks. The midgut was also examined after RNAi using light and transmission electron microscopy. RT-PCR showed differences in gene expression in some organs and developmental stages. Interestingly, only HlFER2 was detected in the ovary during oviposition and in the egg despite the low mRNA transcript. RNAi induced a reduction in post-blood meal body weight, high mortality and decreased fecundity. The expression of vitellogenin genes was affected by silencing of ferritin. Abnormalities in digestive cells, including disrupted microvilli, and alteration of digestive activity were also observed. Taken altogether, our results show that the iron storage and protective functions of ferritin are crucial to successful blood feeding and reproduction of H. longicornis. Supplementary material available online at

show abstract

HeLp, a Heme Lipoprotein from the Hemolymph of the Cattle Tick,Boophilus microplus

Cited by 92 publications

References 50 publications

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Ancestral reconstruction of tick lineages

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Contact Info

Product

Resources

About