HEMA modulates the transcription of genes related to oxidative defense, inflammatory response and organization of the ECM in human oral cells

Perduns, Renke; Volk, Joachim; Schertl, Peter; Leyhausen, Gabriele; Geurtsen, Werner

doi:10.1016/j.dental.2019.01.011

Cited by 16 publications

(13 citation statements)

References 64 publications

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“…Cytotoxicity evaluation is one of the essential tests used to assess the biocompatibility of materials that have to be employed on human beings. Nowadays, despite the undiscussed popularity of dental CRs, there are increasing concerns about the potential cytotoxicity and genotoxicity of the components that these materials may release [ 2 , 9 , 10 , 11 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…This incomplete reaction leads to the release of monomers into the oral cavity during a variable period [ 18 ]. Subsequently, in later stages, biocompatibility can be further influenced by other factors such as erosion, degradation, and the presence of bacteria at the interface between restoration and dental tissues [ 11 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

et al. 2021

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The aim of the study was to evaluate the cytotoxic and genotoxic potential of five commercially available dental composite resins (CRs), investigating the effect of their quantifiable bisphenol-A-glycidyl-methacrylate (Bis-GMA) and/or triethylene glycol dimethacrylate (TEGDMA) release. Experiments were performed using the method of soaking extracts, which were derived from the immersion of the following CRs in the culture medium: Clearfil-Majesty-ES-2, GrandioSO, and Enamel-plus-HRi (Bis-GMA-based); Enamel-BioFunction and VenusDiamond (Bis-GMA-free). Human Gingival Fibroblasts (hGDFs) were employed as the cellular model to mimic in vitro the oral cavity milieu, where CRs simultaneously release various components. Cell metabolic activity, oxidative stress, and genotoxicity were used as cellular outcomes. Results showed that only VenusDiamond and Enamel-plus-HRi significantly affected the hGDF cell metabolic activity. In accordance with this, although no CR-derived extract induced a significantly detectable oxidative stress, only VenusDiamond and Enamel-plus-HRi induced significant genotoxicity. Our findings showed, for the CRs employed, a cytotoxic and genotoxic potential that did not seem to depend only on the actual Bis-GMA or TEGDMA content. Enamel-BioFunction appeared optimal in terms of cytotoxicity, and similar findings were observed for Clearfil-Majesty-ES-2 despite their different Bis-GMA/TEGDMA release patterns. This suggested that simply excluding one specific monomer from the CR formulation might not steadily turn out as a successful approach for improving their biocompatibility.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

et al. 2021

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“…Furthermore, the cytotoxicity of the eluates depends on the nature and the effective quantity of eluted monomers [40]. HEMA‐containing resins have been shown to be highly cytotoxic [41], and this effect was shown to be dose‐dependent [42]. The possible high amount of HEMA elution could also be the reason for the high cytotoxicity of R5 observed in this study.…”

Section: Discussionmentioning

confidence: 90%

Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities

Al-Ani

Salim

Seseogullari‐Dirihan

et al. 2021

European J Oral Sciences

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This study evaluated the cytotoxicity of methacrylate‐based resins containing dimethyl sulfoxide (DMSO). DMSO was incorporated into hydrophobic (R2) and hydrophilic (R5) resins at weight concentrations of 0, 0.01, 0.1, 1, 5, or 10 w/w %. Resin discs (n = 10/group) were prepared. Human gingival fibroblasts (HGF‐1) were exposed to resin eluates for 24 h. Furthermore, dentin barrier test was performed using 3‐D cultures of odontoblast‐like cells (SV40 transfected pulp derived cells) with dentin slices of 400 µm thickness (n = 8). After acid etching of dentin, DMSO‐modified resins were applied into the cavity part of the device and light‐cured for 20 s. Cell viability (%) was assessed by MTT and analyzed spectrometrically. Data were analyzed by ANOVA and Tukey test (α = 0.05). Resin eluates showed statistically significantly lower % cell viability for all neat and DMSO‐modified resins than seen for the negative control. Moreover, DMSO‐R5 eluates resulted in significantly lower % cell viability than DMSO‐R2 emulates. The dentin barrier test showed that DMSO‐R2 did not result in significantly lower % cell viability, whereas incorporation of 1‐10 w/w % DMSO into R5 resulted in significantly lower % of cell viability. Incorporating DMSO into hydrophilic self‐etching resins may increase cytotoxicity. The biocompatibility is not influenced by the addition of DMSO into hydrophobic resin.

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“…Since inflammation is known to influence ECM organization [54] we monitored the effects of adhesive extracts on transcription of ECM elements.…”

Section: Discussionmentioning

confidence: 99%

Morpho-functional effects of different universal dental adhesives on human gingival fibroblasts: an in vitro study

et al. 2020

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To analyze the effects of four universal adhesives (Optibond Solo Plus—OB, Universal Bond—UB, Prime&Bond Active—PBA, FuturaBond M + —FB) on human gingival fibroblasts in terms of cytotoxicity, morphology and function. After in vitro exposure for up to 48 h, fibroblast viability was determined by the MTT assay determined, morphology by phase-contrast microscopy and migration by the scratch wound assay. Expression levels of IL1β, IL6, IL8, IL10, TNFα and VEGF genes were assessed by RT-PCR and their protein production by Western blot analysis. Apoptosis and cell cycle were analyzed by flow cytometry. OB and UB induced early morphological changes on fibroblasts (3 h) with extended cell death at 24 h/48 h. Gene expression of collagen type I and fibronectin increased fivefold compared with controls, elastin disappeared and elastase increased threefold, indicating gingival tissue tended to become fibrotic. Only UB and OB increased gene expression of inflammatory markers: IL1β at 3 and 48 h (up to about three times), IL6 and IL8 at 3 h (up to almost four times) which corresponded to the increase of the activated form NF-kB. All adhesives showed an effect on the functionality of fibroblasts with cytotoxic effect time and concentration dependent. Among all the OB and UB adhesives, they showed the greatest cell damage. The in-depth analysis of the effects of universal adhesives and possible functional effects represents an important information for the clinician towards choosing the most suitable adhesive system.

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HEMA modulates the transcription of genes related to oxidative defense, inflammatory response and organization of the ECM in human oral cells

Cited by 16 publications

References 64 publications

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

Cytotoxic and Genotoxic Effects of Composite Resins on Cultured Human Gingival Fibroblasts

Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities

Morpho-functional effects of different universal dental adhesives on human gingival fibroblasts: an in vitro study

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