Hemagglutinating Activity of Serovar Reference Strains of <i>Ornithobacterium Rhinotracheale</i>

Vega, V.; Zepeda, A.; Ramirez, Saul; Morales-Erasto, Vladimir; Fernandez, Pomposo; de, Roberto Montes; Guerra‐Infante, Fernando M.; Haro-Cruz, María de Jesús de; Blackall, P. J.; Soriano, Edgardo V.

doi:10.1177/104063870802000317

Cited by 6 publications

(8 citation statements)

References 4 publications

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“…The hemagglutinating activity of all stable O. rhinotracheale SCVs and WT isolates was tested with fresh chicken, pigeon, and rabbit erythrocytes, as described previously (15,34). Briefly, fresh bacterial suspensions of the O. rhinotracheale isolates were prepared from 48-h cultures on SBA; they were harvested in 1.5 ml of PBS (pH 7.0), adjusted to an OD 600 value of 1.5, and washed 3 times with PBS.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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bOrnithobacterium rhinotracheale is a Gram-negative bacterium associated with respiratory diseases in many avian species, with worldwide distribution, and it causes significant economic loss to the poultry industry. In this study, the isolation and characterization of O. rhinotracheale small-colony variants (SCVs) are described for the first time. O. rhinotracheale isolates (n ‫؍‬ 27) were recovered from tracheal samples (n ‫؍‬ 321) collected from different avian species with clinical signs of respiratory disease. Of the 27 O. rhinotracheale isolates, 21 (77.8%) showed SCVs in their primary cultures. Five O. rhinotracheale SCV isolates showed high levels of stability and were chosen for further characterization with their wildtype (WT) isolates. Stable O. rhinotracheale SCVs were oxidase negative, while their WT isolates were positive. Growth curves for stable O. rhinotracheale SCVs indicated lower growth rates and longer lag phases than for their WT isolates. Furthermore, it was possible to increase the efficacy of the broth medium in supporting the growth of O. rhinotracheale WT isolates by supplementing it with 5% fetal bovine serum (FBS) and 2% IsoVitaleX Enrichment. Antibiotic susceptibility tests showed that O. rhinotracheale SCVs had higher MIC values than their WT isolates. This study suggests that successful antibiotic treatment of respiratory diseases associated with O. rhinotracheale must take into consideration the resistance patterns of O. rhinotracheale SCVs. Intracellular persistence in murine RAW 264.7 macrophages revealed that O. rhinotracheale SCV28 had higher survival rates than its WT isolate. Finally, small-colony variants may be important contributors to the pathogenesis of O. rhinotracheale.

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Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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“…There are 18 serotypes for ORT, with the majority of chicken isolates belonging to four serotypes A, B, D and E (Chansiripornchai et al 2007;Thachil et al 2007). Haemagglutinating activity of ORT serovars has been studied by using fresh erythrocytes from different species (Vega et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

Ghanbarpour

Salehi

2009

Trop Anim Health Prod

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Ornithobacterium rhinotracheale is a gram negative bacterial pathogen causing respiratory tract infections in poultry. Tracheal, lung and serum samples were obtained from 21 broiler flocks of 8 farms from a slaughterhouse located in south-eastern of Iran. Among 630 tracheal and lung samples from samples resulting from 315 chickens, 11 (3.5%) ORT isolates were identified using biochemical tests. The isolates originated from 9 (42.9%) flocks out of 4 farms. All of the isolates were recovered from tracheal swabs and showed an API 20NE identification biocode 0-2-2-0-0-0-4. Of the 420 serum samples examined by ELISA, 134 (31.9%) sera from 17 (81.0%) flocks were positive for ORT antibodies. These results indicate that ORT is present in most broiler flocks with respiratory disorders in southeast Iran.

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“…Five ORT reference strains were used, corresponding to serovars A, B, C, D, and E (Table 1). All the strains were cultured on 5% ovine blood agar (DIBICO, Mexico City, Mexico) and further incubated at 37°C in 5% CO 2 for 48 h (Soriano et al, 2003, Vega et al, 2008. S. mutans ATCC 25125 was used as a positive control of biofilm-producing bacteria, and was cultured in the same medium and under the same culture conditions as ORT (Yoshida & Kuramitsu, 2002).…”

Section: Methodsmentioning

confidence: 99%

“…This bacterium is able to adhere to tracheal cells in chickens and possesses a haemagglutinin and a neuraminidase (Soriano et al, 2003;Vega et al, 2008;De Haro-Cruz et al, 2013;Kastelic et al, 2013); additionally, it has been reported that some ORT isolates are able to produce a biofilm (Vichi et al, 2012). A biofilm is considered to be a complex microbial community where microorganisms synthesize an exopolysaccharide matrix that protects the microorganisms against the environment, antimicrobial agents and the immune response of the host, it can adhere to either an inert surface or a live tissue (Lasa et al, 2005;Vila et al, 2008;Castrillón et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

Rosa-Ramos¹,

Rodríguez-Cruz

López-Villegas

et al. 2015

Avian Pathology

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Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.

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Hemagglutinating Activity of Serovar Reference Strains of Ornithobacterium Rhinotracheale

Cited by 6 publications

References 4 publications

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

Conditions that induce biofilm production byOrnithobacterium rhinotracheale

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