Hemagglutinating Property of Haemophilus aegyptius

Mann, Jerry M.; Rheins, Melvin S.

doi:10.1128/aem.16.9.1343-1347.1968

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…In 1949, Davis et al reported that 27 of 28 different strains of H. influenzae biogroup aegyptius agglutinated human erythrocytes (12). In a subsequent study, Mann and Rheins succeeded in isolating an H. influenzae biogroup aegyptius hemagglutinin by extraction of whole bacteria with either diethylene glycol or acetone; chemical analysis indicated the absence of any protein content and identified the hemagglutinin as lipopolysaccharide (23). In our collection of eight strains, we identified two that were capable of hemagglutination via a protease-resistant mechanism.…”

Section: Discussionmentioning

confidence: 99%

Surface structures and adherence properties of diverse strains of Haemophilus influenzae biogroup aegyptius

1991

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Haemophilus influenzae biogroup aegyptius is an important cause of conjunctivitis and has recently been associated with Brazilian purpuric fever (BPF), a fulminant systemic disease of children. To gain insight into the bacterial factors involved in the pathogenesis of this disease, we investigated the surface structures and adherence properties of eight different strains of H. influenzae biogroup aegyptius, including both BPF and non-BPF isolates. All eight strains were able to express long peritrichous pili similar to those observed in H. influenzae. As in H. influenzae, piliation correlated with colony binding of human erythrocytes. However, two strains were capable of hemagglutination in the absence of pili; in these strains, hemagglutination was insensitive to protease treatment, suggesting a nonproteinaceous hemagglutinin. All strains possessed short, thin, surface fibers distinct from long pili and demonstrated efficient attachment to cultured human conjunctival cells. Attachment to conjunctival cells occurred independently of long pili or a capacity for hemagglutination.

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Section: Discussionmentioning

confidence: 99%

Surface structures and adherence properties of diverse strains of Haemophilus influenzae biogroup aegyptius

1991

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“…A 1956 review by Neter (12) lists 17 bacterial species that aggregate red blood cells (RBC). Scattered reports in more recent years have greatly extended this list to include Listeria monocytogenes (9), Haemophilus influenzae (8), H. aegyptius (10), Proteus strains (2), Shigella flexneri (11), Diplococcus pneumoniae (13), Clostridiwn welchil (3), and Streptococcus agalactiae (14). A practical application of bacterial HA was reported by Finkelstein and Mukerjee (7), who differentiated Vibrio comma from El Tor vibrios with a rapid slide test.…”

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confidence: 99%

Hemagglutinin from the L Phase of Neisseria meningitidis Group B, with Observations on a Serum Inhibitor

et al. 1971

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A hemagglutinin extracted from the L-phase of Neisseria meningitidis group B directly agglutinated rabbit and rooster erythrocytes but did not react with human, sheep, or guinea pig erythrocytes. Virtually all animal, chicken, and human sera tested showed inhibitory titers. In a significant number of Navy recruits, who became carriers of N. meningitidis, the titer of serum inhibitor began to decline 2 weeks before the bacterial infection was first detected. Titers of this serum inhibitor ranged from 256 to 4,096 in patients with meningitis. The hemagglutinin failed to boost the preexisting titers of serum inhibitor when injected into animals, although roosters showed a minimal but transitory response.

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