Hemagglutination by Staphylococcus aureus strains responsible for human bacteremia or bovine mastitis

Rupp, Mark E.; Han, Jenny; Gatermann, Sören

doi:10.1007/bf00216787

Cited by 9 publications

(11 citation statements)

References 8 publications

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“…While we found that all tested S. aureus strains possessed the tested region of the ica locus, we were unable to demonstrate evidence for PIA production in vitro, using bio®lm assay, hemagglutination assay, and two dierent kinds of growth conditions. This ®nding is consistent with most previous investigations evaluating the presence of the ica locus in S. aureus [4,17,21]. For example, it was recently shown that, although S. aureus synthesized PNSG as a surface polysaccharide during human and animal infection, few strains expressed PNSG in vitro [17].…”

Section: Discussionsupporting

confidence: 86%

“…In previous experiments we have demonstrated that hemagglutination in S. epidermidis is mediated by PIA [5,15]. We documented that 8 of 70 (13%) S. aureus strains responsible for bactere- [21]. In this report we add to the body of evidence demonstrating that ica is present in S. aureus by analyzing a collection of prospectively identi®ed clinical strains.…”

Section: Discussionsupporting

confidence: 52%

“…Because these clinical isolates were frozen immediately after isolation of S. aureus from a blood culture vial, only two passages were necessary prior to analysis. S. aureus strain S680, a previously described strain responsible for bovine mastitis, was used as a positive control strain in the hemagglutination and bio®lm assays [21].…”

Section: S Aureus Strainsmentioning

confidence: 99%

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The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

Fowler

Fey

Reller

et al. 2001

Medical Microbiology and Immunology

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Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 52%

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The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

Fowler

Fey

Reller

et al. 2001

Medical Microbiology and Immunology

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“…In another study, agglutination ratio was determined as 33% for coagulase negative staphylococci except S. epidermidis [9] . The haemagglutination feature of S. aureus was found in the ratio of 23% in a study performed previously [26] . In this study, haemagglutination ratio was determined in the ratio of 14.28% for coagulase positive Staphylococci and 16.32% for coagulase negative Staphylococci.…”

Section: It Has Been Shown That Cns (S Haemolyticus S Hyicus S Smentioning

confidence: 77%

Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi

Savaşan¹,

Kırkan²,

Erbaş³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleSavaşan S, Kırkan Ş, Erbaş G, Parın U, Çiftci A: Determination of virulence factors of Staphylococci isolated from bovine mastitis. Kafkas Univ Vet Fak Derg, 23 (6): 947-952, 2017. DOI: 10.9775/kvfd.2017 AbstractStaphylococcal mastitis is one of the major economic problems of cattle. The aim of this study was to identify Staphylococcus species that cause mastitis, to examine the virulence factors of these species and to determine the relation between these factors with the pathogenic and nonpathogenic species. In the study, 37 S. aureus, 13 S. hyicus, 9 S. simulans, 8 S. chromogenes, 5 S. lentus, 5 S. epidermidis, 2 S. haemolyticus, 2 S. hominis, 1 S. auricularis, 1 S. warneri, 1 S. sciuri were isolated and identified. The 41.6% of strains were determined as coagulase positive. In the coagulase positive strains, the rate of protein A, DNase, TNase, capsul, hemolyse, staphylokinase, slime (in agar), biofilm (microdilution) and hemagglutination characteristics were found 71.4%, 48.6%, 11.4%, 40%, 97.1%, 40%, 28.6%, 37.1% and 17.1%, respectively. In the coagulase negative strains, the rate of these characteristics were found 10.2%, 12.2%, 2%, 8.2%, 82%, 32.7%, 12.2%, 12.2% and 10.2%, respectively. The methicillin resistance rates in the coagulase positive and negative strains were determined as 2.9 and 16.3%. In conclusion, it was of the opinion that animals are potential carriers of staphylococcus strains that are pathogen for human. Keywords: Bovine, Mastitis, Staphylococci, Virulence Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi ÖzetStafilokokal mastitisler sığırlar için en önemli ekonomik problemlerden birisidir. Bu çalışmada mastitise neden olan stafilokok türlerinin sahip olduğu virülens faktörlerinin belirlenmesi ve patojenik ile apatojenik türler arasında bu faktörlerin ilişkisinin araştırılması amaçlanmıştır. Çalışma kapsamında 37 S. aureus, 13 S. hyicus, 9 S. simulans, 8 S. chromogenes, 5 S. lentus, 5 S. epidermidis, 2 S. haemolyticus, 2 S. hominis, 1 S. auricularis, 1 S. warneri ve 1 S. sciuri izole ve identifiye edilmiştir. Bu izolatların %41.6'sı koagülaz pozitif olarak belirlenmiştir. Koagülaz pozitif izolatların protein A, DNase, TNase, kapsül, hemoliz, stafilokinaz, slaym (agar'da), biyofilm (mikrodilüsyon) ve hemaglütinasyon özellikleri sırasıyla %71.4, %48.6, %11.4, %40, %97.1, %40, %28.6, % 37.1 ve %, 17.1 olarak tespit edilmiştir. Koagülaz negatif izolatlarda ise bu oranlar sırasıyla %10.2, %12.2, %2, %8.2, %82, %32.7, %12.2, %12.2 ve %10.2 bulunmuştur. Koagülaz pozitif ve negatif izolatlar arasında metisilin direnç oranı %2.9 ve %16.3 olarak saptanmıştır. Sonuç olarak, stafilokok taşıyıcısı hayvanların insanlar için muhtemel infeksiyon kaynağı olabileceği sonucuna varılmıştır.

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“…It was reported that more than 75% of S. aureus strains causing mastitis mediated by haemaglutination. Haemaglutination also related to S. aureus adherence in human epithelial cells (Rupp et al, 1995).…”

Section: Resultsmentioning

confidence: 96%

Determination of Haemaglutinin and Gene Encoding Fibronectin Binding Proteins Staphylococcus aureus Isolated from Dairy Milk Cows

Pratomo

Salasia

Tato

2015

Indones. J. Biotechnol.

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Staphylococcus aureus is a major pathogen causing clinical and subclinical mastitis in dairy milk cows. The mastitis has immense economical impacts, where it reduces of the quantity and quality of milk production. The aims of the research were to analyse haemaglutinin and gene encoding fibronectin binding proteins. Nineteen Staphylococcus aureus isolates used in the present study were isolated from dairy milk cows from Yogyakarta, Solo, Boyolali and Sumedang. The haemagluitinin of S. aureus were determined based on haemaglutination reaction to erythrocytes of rabbit. Detection of gene encoding fibronectin binding proteins could be performed with specific primers using polymerase chain reaction (PCR). The results of studies showed that most of S. aureus (78,95%) expressed haemaglutinin based on their ability to aglutinate rabbit erythrocytes. Analysis of gene encoding fibronectin binding proteins of S. aureus revealed gene fnbA with size of approximately 1300 bp for 57,89% isolates, gene fnbB with size of approximately 900 bp for 31,58% isolates and both of gene fnbA and fnbB could be detected for 31,58% isolates. The characters of S. aureus based on haemaglutinin, gene fnbA and fnbB of the present study could be used as an information to control of S. aureus infection in dairy herds.

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Hemagglutination by Staphylococcus aureus strains responsible for human bacteremia or bovine mastitis

Cited by 9 publications

References 8 publications

The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi

Determination of Haemaglutinin and Gene Encoding Fibronectin Binding Proteins Staphylococcus aureus Isolated from Dairy Milk Cows

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