Hemangioendothelioma of Bone in a Patient with a Constitutional Supernumerary Marker

Rogatto, Sílvia Regina; Rainho, Cláudia Aparecida; Zhang, Z. M.; Figueiredo, Francisco C; Barbieri‐Neto, José; Georgetto, S. M.; Squire, Jeremy A.

doi:10.1016/s0165-4608(98)00192-7

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…A further requirement for clonality was that the changes had to be found in at least two culture flasks. FISH was performed as described previously (Rogatto et al, 1999;Dracopolli, 2000). In brief, the slides were treated with RNase 1x for 1 h at 37°C.…”

Section: Cytogenetic Studiesmentioning

confidence: 99%

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

et al. 2003

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Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.

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Section: Cytogenetic Studiesmentioning

confidence: 99%

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

et al. 2003

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“…Accumulation of similar data may help to clarify the relationship of sSMC21 and cancer onset, which in the present case is likely to be a mere coincidence. sSMC have been reported for various chromosomes namely 15 (Schmid et al, 1986;Adhvaryu et al, 1998;Roberts et al, 2002), 8 (Rothenmund et al, 1997Batanian et al, 2000), 22 (Rogatto et al, 1999), and X (Tumer et al, 1998). Adhvaryu et al (1998) reported a familial supernumerary marker chromosome 47,XY,+r(15) that was likely to be maternally inherited.…”

Section: Discussionmentioning

confidence: 99%

“…A case of hemangioendothelioma of bone has been reported in a patient with a constitutional sSMC involving chromosomal region 22q11. The chromosome was identified on the basis of conventional FISH, as spectral karyotyping was non-informative (Rogatto et al, 1999). Another patient with a constitutional bisatellited supernumerary chromosome developed lung carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a familial small supernumerary marker chromosome in a patient with adult-onset tongue cancer

Bakshi

Davé

Sanger

et al. 2008

Cytogenet Genome Res

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Cytogenetic analysis in peripheral blood lymphocytes of a 50-year-old female with tongue cancer showed the presence of one to three copies of a small supernumerary marker chromosome (sSMC) in a mosaic state. Family studies also revealed the marker in mosaic form in four (age <29 years) of eleven clinically normal individuals studied from her family of 16 individuals spanning three generations. Due to the extremely small size of the marker chromosome, identification by classical cytogenetics was not informative. Multicolor FISH followed by whole chromosome painting identified the marker as a derivative of chromosome 21. This is the first report of sSMC21 in an adult-onset tongue cancer patient and some of her family members with no clinical symptoms.

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“…Given the fact that hAB cells are genetically unstable and exhibit a huge number of chromosomal deletions, insertions, and duplications as well as small supernumerary marker chromosomes (38,39), it seems probable that a prolonged period of cultivation might positively select for the fastest-growing and potentially more malignant phenotypes.…”

Section: Fig 4 Representative Images From Parental Hmscs and Hab Cellmentioning

confidence: 99%

Efficient Transformation of Primary Human Mesenchymal Stromal Cells by Adenovirus Early Region 1 Oncogenes

Speiseder

Hofmann-Sieber

Rodríguez

et al. 2017

J Virol

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Previous observations that human amniotic fluid cells (AFC) can be transformed by human adenovirus type 5 (HAdV-5) E1A/E1B oncogenes prompted us to identify the target cells in the AFC population that are susceptible to transformation. Our results demonstrate that one cell type corresponding to mesenchymal stem/stroma cells (hMSCs) can be reproducibly transformed by HAdV-5 E1A/E1B oncogenes as efficiently as primary rodent cultures. HAdV-5 E1-transformed hMSCs exhibit all properties commonly associated with a high grade of oncogenic transformation, including enhanced cell proliferation, anchorage-independent growth, increased growth rate, and high telomerase activity as well as numerical and structural chromosomal aberrations. These data confirm previous work showing that HAdV preferentially transforms cells of mesenchymal origin in rodents. More importantly, they demonstrate for the first time that human cells with stem cell characteristics can be completely transformed by HAdV oncogenes in tissue culture with high efficiency. Our findings strongly support the hypothesis that undifferentiated progenitor cells or cells with stem cell-like properties are highly susceptible targets for HAdV-mediated cell transformation and suggest that virus-associated tumors in humans may originate, at least in part, from infections of these cell types. We expect that primary hMSCs will replace the primary rodent cultures in HAdV viral transformation studies and are confident that these investigations will continue to uncover general principles of viral oncogenesis that can be extended to human DNA tumor viruses as well.IMPORTANCE It is generally believed that transformation of primary human cells with HAdV-5 E1 oncogenes is very inefficient. However, a few cell lines have been successfully transformed with HAdV-5 E1A and E1B, indicating that there is a certain cell type which is susceptible to HAdV-mediated transformation. Interestingly, all those cell lines have been derived from human embryonic tissue, albeit the exact cell type is not known yet. We show for the first time the successful transformation of primary human mesenchymal stromal cells (hMSCs) by HAdV-5 E1A and E1B. Further, we show upon HAdV-5 E1A and E1B expression that these primary progenitor cells exhibit features of tumor cells and can no longer be differentiated into the adipogenic, chondrogenic, or osteogenic lineage. Hence, primary hMSCs represent a robust and novel model system to elucidate the underlying molecular mechanisms of adenovirus-mediated transformation of multipotent human progenitor cells.

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Hemangioendothelioma of Bone in a Patient with a Constitutional Supernumerary Marker

Cited by 15 publications

References 23 publications

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

Characterization of a familial small supernumerary marker chromosome in a patient with adult-onset tongue cancer

Efficient Transformation of Primary Human Mesenchymal Stromal Cells by Adenovirus Early Region 1 Oncogenes

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