Hematogones Detected by Flow Cytometry in a Child with Vitamin B12 Deficiency

Sutton, Lisa; Nkechi,

doi:10.1177/1093526616686241

Cited by 3 publications

(7 citation statements)

References 17 publications

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“…acute leukemias, myelodysplastic syndromes, etc). This requires an invasive study, as in our series 5 . Flow cytometry is a complementary diagnostic tool with short-term results in this diagnostic process, especially in cases where morphology is not specific and there is no permanent availability for measuring plasma levels of vitamin B12, as in our center.…”

Section: Discussionmentioning

confidence: 86%

“…In patients with typical clinical and laboratory manifestations, low serum levels of vitamin B12 allow us to confirm the diagnosis. Other tests used are methylmalonic acid and homocysteine levels, with the last being more specific; however, both studies are not available in most centers and can be altered by other factors 3 , 5 , 10 . Nevertheless, in some cases severe cytopenias appear, forcing us to rule out other causes of marrow failure (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…The morphological findings include hypercellular bone marrow, with an increase in the size of the hematopoietic precursors due to asynchrony in maturation between the nucleus and cytoplasm. These characteristics must be interpreted cautiously due to overlapping with those observed in other pathologies such as myelodysplastic syndromes 5 .…”

Section: Introductionmentioning

confidence: 93%

“…Los hallazgos morfológicos incluyen una médula ósea hipercelular, con aumento del tamaño de los precursores hematopoyéticos en la médula ósea y asincronía madurativa núcleo-citoplasmática. Estas características deben ser interpretadas con cuidado, debido a que se solapan con las que se pueden observar en otras patologías como los síndromes mielodisplásicos 5 .…”

Section: Introductionunclassified

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Cambios detectados por citometría de flujo en la anemia megaloblástica secundaria a la deficiencia de cobalamina

Briones Muñoz,

Figueroa Faúndez,

Vidal Rojas

et al. 2023

Colomb Med

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Introducción:Anemias megaloblásticas secundarias a la deficiencia de vitamina B12 son patologías producidas por una síntesis defectuosa del ADN nuclear. Objetivo:Describir las alteraciones madurativas encontradas en precursores hematopoyéticos de la médula ósea de una serie de pacientes con anemia megaloblástica. Métodos:Se incluyeron pacientes atendidos en el Hospital Regional de Concepción con muestras de médula ósea enviadas para estudio de citopenias por citometría de flujo cuyo diagnóstico fue anemia megaloblástica. El inmunofenotipo se realizó con CD45, CD34, CD117, HLA-DR, marcadores de maduración de serie de neutrófilo (CD13, CD11b, CD10, CD16) y/o eritroblasto (CD105, CD71, CD36). Resultados:Se identificaron 8 pacientes con anemia megaloblástica y como controles se utilizaron síndromes mielodisplásicos (n=9) y médula ósea normal o reactiva (n=10). El 44% eran hombres, con una mediana de edad de 58 años. La anemia megaloblástica se asoció con una mayor proporción de tamaño y complejidad de progenitores eritroides y mieloides con respecto de los linfocitos en comparación a los controles. El porcentaje total de eritroblastos y la proporción de células mieloides CD34+ comprometidas con el linaje eritroide fue mayor en anemia megaloblástica, asociado a una parada madurativa en la etapa de precursor CD105+ (69% vs 19% y 23%, p <0.001). La heterogeneidad de CD36 y CD71 en anemia megaloblástica fue similar a los síndromes mielodisplásicos Conclusiones:La anemia megaloblástica produce una afectación heterogénea de la hematopoyesis, caracterizada por un mayor tamaño y complejidad celulares de precursores de la serie neutrófilo y eritroide y una detención madurativa de los eritroblastos

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionunclassified

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Cambios detectados por citometría de flujo en la anemia megaloblástica secundaria a la deficiencia de cobalamina

Briones Muñoz,

Figueroa Faúndez,

Vidal Rojas

et al. 2023

Colomb Med

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“…72,73 The evidence of increased hematogones in severe CD may differentiate nutritional from malignant etiology of BMF. 74…”

Section: Pa Presenting With Bone Marrow Failure-like Syndromementioning

confidence: 99%

Pernicious anemia: Pathophysiology and diagnostic difficulties

Htut

Thein

2021

J Evidence Based Medicine

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Pernicious anemia (PA) is the most common cause of vitamin B12 (cobalamin) deficiency anemia in the world. It is an autoimmune disease, comprising of salient features of autoimmune chronic atrophic gastritis (CAG) and cobalamin deficiency (CD).Although the anemia was first described as pernicious, it may well be controlled with vitamin B12 replacement. The onset and progression of PA is often insidious. Alternatively, patients may have no anemic symptoms since they become acclimatized to the subtle nature of the disease. Oftentimes, there is a possibility that the underlying disease may be missed unless a full blood count (FBC) is investigated, leading to hindrance in the treatment journey. Diagnostic challenges remain tangible for many practicing clinicians, since there is lack of reliable cobalamin assays to diagnose CD as well as clinical mimics, which simulate many other hematological conditions, such as myelodysplastic syndrome, acute leukemia, sideroblastic anemias, bone marrow failure states, thrombotic microangiopathy, and thromboembolism. Moreover, prompt recognition of the symptoms of CD is also vital, because some neurologic sequalae may become irreversible despite replenishing cobalamin. Herein, we discuss a literature review on the pathophysiology, challenging clinical presentations and diagnostic difficulties of PA.Since the cobalamin replacement therapy for PA is straightforward, it will not be discussed in this review.

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Targeting the sulfur-containing amino acid pathway in leukemia

Chen,

Jin,

Chang

et al. 2024

Amino Acids

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Abstractsulfur-containing amino acids have been reported to patriciate in gene regulation, DNA methylation, protein synthesis and other physiological or pathological processes. In recent years, metabolism-related molecules of sulfur-containing amino acids affecting the occurrence, development and treatment of tumors have been implicated in various disorders, especially in leukemia. Here, we summarize current knowledge on the sulfur-containing amino acid metabolism pathway in leukemia and examine ongoing efforts to target this pathway, including treatment strategies targeting (a) sulfur-containing amino acids, (b) metabolites of sulfur-containing amino acids, and (c) enzymes and cofactors related to sulfur-containing amino acid metabolism in leukemia. Future leukemia therapy will likely involve innovative strategies targeting the sulfur-containing amino acid metabolism pathway.

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Hematogones Detected by Flow Cytometry in a Child with Vitamin B12 Deficiency

Cited by 3 publications

References 17 publications

Cambios detectados por citometría de flujo en la anemia megaloblástica secundaria a la deficiencia de cobalamina

Cambios detectados por citometría de flujo en la anemia megaloblástica secundaria a la deficiencia de cobalamina

Pernicious anemia: Pathophysiology and diagnostic difficulties

Targeting the sulfur-containing amino acid pathway in leukemia

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