Hematological Changes in Mice Following Freund’s
Adjuvant Administration*

Morton, Jane I.; Siegel, B

doi:10.1159/000465159

Cited by 3 publications

(3 citation statements)

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“…The anaemia is hypochromic and macrocytic and is accompanied by rather high reticulocytosis. Leucocytosis is due mainly to an increase in polymorphonuclear granulocytes, but the absolute number of lymphocytes is also augmented, in contrast to the lymphopenia observed in mice in the early stages of adjuvant-induced disease (Morton and Siegel, 1966). The thrombocyte counts do not differ from those in the controls.…”

Section: Resultsmentioning

confidence: 77%

Haematological changes in adjuvant disease in the rat. I. Peripheral blood and bone marrow after repeated injections of Freund's adjuvant.

Mikolajew¹,

Kuratowska²,

Kossakowska³

et al. 1969

Annals of the Rheumatic Diseases

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Section: Resultsmentioning

confidence: 77%

Haematological changes in adjuvant disease in the rat. I. Peripheral blood and bone marrow after repeated injections of Freund's adjuvant.

Mikolajew¹,

Kuratowska²,

Kossakowska³

et al. 1969

Annals of the Rheumatic Diseases

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“…However, it was noted in the present experiments that body weights of adjuvant-immunized mice on heavy water were lower than those of immunized animals drinking H20. These differences could be ascribed to the marked proliferation of tissues of the reticuloendothelial system (RES) that occurs in the adjuvant-injected mouse, resulting in an increase in body weight (9). In the case of immunized animals on DzO, this weight increase was less marked.…”

Section: Influence Of Deuteration On Circulating Antibody Levels In Thementioning

confidence: 99%

Influence of Deuteration on Circulating Antibody Levels in the Mouse.

Siegel

Morton

1966

Experimental Biology and Medicine

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The isotopic substitution of deuterium for hydrogen has been shown to markedly alter the relative rates of chemical reactions and to affect the conformation and stability of macromolecules ( 1 ) . The profound physiological and biological changes associated with high degrees of deuteration have been found not to be compatible with survival in the mammalian organism( 2). However, the continuous ingestion with 30% deuterium oxide (DzO, heavy water) appears to have no appreciable effects on the body weights, general well-being or life span of the laboratory mouse(3). We have recently demonstrated the protective effects of this, and lower levels of D 2 0 on mice infected with the Rauscher leukemogenic virus (4). The mechanism of protection appeared to be depression of the neoplastic proliferation of lymphoreticular cells. In view of similarities noted between cells undergoing leukemic proliferation and those responding immunologically ( 5) it seemed feasible to investigate the effects of deuteration on the immune response.Matwials and methods. Deuterium oxide (supplied as 99.7% DzO by the Richland Operations Office of the U. S. Atomic Energy Commission, Richland, Wash.) was purified by distillation from alkaline permanganate. BALB/c/jax female mice, 5-7 weeks old, were fed a standard pellet diet and deuterium was administered as drinking water ad libitum at a concentration (v/v) of 30% for the duration of the experiments.Mice received a single intraperitoneal injection of 1.25 mg bovine serum albumin (BSA, crystalline, Pentex, Inc., Kankakee, Ill.) in 0.25 ml of a complete Freund's adjuvant emulsion (Difco Laboratories, Detroit) either 10 days or 17 days after they started drinking heavy water. Sheep erythrocytes were washed 3 times in 20 volumes of cold saline and the packed cells suspended in *Sumpported by U. S. Atomic Energy Commission Contract RLO-192 '1-3. 2 volumes of saline for intraperitoneal injection either as a single dose of 0.25 ml or as two 0.25 ml inoculations 18 hours apart. Mice were bled periodically from the retro-orbital plexus. Antibody titers to BSA were determined by the agglutination of tanned, antigencoated sheep erythrocytes as described by Stavitsky (6). Red cell agglutinins were determined directly in a 2-fold serial dilution system in the presence of 1:200 saline diluted normal rabbit serum. To assay macroglobulin agglutinin activity, aliquots of mouse antisera were incubated for 18 hours at 25°C in phosphate buffered saline, pH 7.4, containing 0.1 M 2-mercaptoethanol(7). Serial dilutions and assays were carried out directly in this medium. Statistical significance of data was determined by student t-test analysis of reciprocal logarithmic (base 2) titer values. Both BSA-adjuvant and sheep erythrocyte immunization experiments were repeated twice using 5-8 mice in each group with similar results as reported below.Results. The antibody response of mice drinking 30% D20 is illustrated in Fig. 1. Mice drinking heavy water continuously starting 10 days in advance of immunization showed no sig...

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“…Freund's adjuvant alone and to a lesser degree some of its components produce wide spread granulomata; hyperplasia of reticuloendothelial, vascular, and connective tissues; glomerular lesions (7-9) ; amyloidosis ,and hematological changes in mice (7,10) ; and arthritis in rats (4).…”

mentioning

confidence: 99%