Hematological Profile of Iron Overload in Rats Administered with Fruit Extract of Mahkota Dewa (Phaleria macrocarpa)

Abstract

Background: Repeated blood transfusions lead to an accumulation of iron that exceeds the body's iron storage capacity. Free iron is able to catalyze the formation of hydroxyl radicals that cause oxidative damage and cell death. The use of iron chelating medicines for iron chelation therapy is limited by side effects. Mangiferin is a natural bioactive compound with iron chelating and antioxidant activity. Mahkota dewa is a plant native to Indonesia that contains mangiferin. Objectives: The aim of this study was to evaluate the effect of mahkota dewa fruit extract administration on hematological profile of iron overload rats. Material and Methods: Thirty rats were divided into 6 groups: normal rats (N), iron overload rats (IO), iron overload rats rats given deferiprone (D), mangiferin (M), Mahkota dewa fruit extract at a dose of 100 mg/kg BW (PM1), or 200 mg/kg BW (PM2). Rats were given injection of iron sucrose 15 mg every 3-4 days for 8 weeks. At week 8, rats were sacrificed. Hematological analysis was performed in this study. Results: Iron overload condition caused by iron sucrose injection did not cause statistically significant changes in the haematological profile. Likewise, the administration mahkota dewa fruit extract at a dose of 100 or 200 mg/kg BW did not cause changes in the hematological profile. Conclusions: The administration of mahkota dewa fruit extract did not alter hematological profile of iron oveload rats.

Iron-Overload Conditions: Manifestations to the Kidney Organs – A Review

“…Increased iron absorption in the small intestinal mucosa and frequent red blood cell transfusions can lead to iron overload in patients with chronic anemia such as β-thalassemia major. Before recently, long-term iron chelation therapy with iron chelating medications was the standard for managing iron overload, particularly in patients with transfusion-dependent thalassemia 1 . Hemosiderosis is an iron overload condition with increased iron deposition in tissues without clinical signs 2 .…”