Background: Pre-eclampsia (PE) contributes to the second cause of maternal death in Indonesia. Andaliman is a typical spice of the Batak ethnic in Northern Sumatera Province, Indonesia. This study aimed to explore the potential of novel herbal medicine compound of nanoherbal andaliman and extra virgin olive oil (EVOO) as PE treatment. Methods: Nanoherbal andaliman was generated using High-energy Milling (HEM). The treatments were divided into the following five groups: K- (control): pregnant rats; K+: PE model rats; P1: PE model rats + 0.45 g of EVOO/200 g BW on the 13th–19th day of pregnancy; P2: PE model rats + nanoherbal andaliman 100 mg/200 g BW on the 13th– 19th day of pregnancy; and P3: PE model rats + combination of 0.45 EVOO/200 g BW and nanoherbal andaliman 100 mg/200 g BW on the 13th–19th day of pregnancy. Rats were dissected on the 20th day of pregnancy. The observed parameters were blood pressure, proteinuria, malondialdehyde (MDA), Heat Shock Protein-70 HSP-70 and histology of placenta. Results: A significant difference was noticed (p<0.05) in blood pressure, proteinuria, foetal weight, haematocrit, erythrocytes and trophoblastic cells after the administration of combined nanoherbal andaliman and EVOO. No significant differences in placental weight, foetal number, leukocytes, MDA and HSP-70 were found (p>0.05). Conclusion: The combination of nanoherbal andaliman and EVOO decreased systolic blood pressure and induced the expression of MDA and HSP-70, as well as placental histology of pre-eclamptic rats.

Introduction: Rhodomyrtus tomentosa (haramonting), a typical plant of North Sumatera, Indonesia, contains important medicinal ingredients. Nano sized drugs have high loading capacities and can be given at high concentrations. This study aimed to determine the components and toxicity of nanoherbal haramonting. It also aimed to determine the effect of nanoherbal haramonting on the histology of the liver, kidneys, lungs, heart and brain. Methods: High-energy milling was performed to produce nanoherbal haramonting. Thin-layer chromatography was utilised to determine the chemical components of the nanoherb. Antioxidant tests were performed by using the 1,1-diphenyl-2-picryhydrazil method. The three-stage of lethal dose 50 (LD50) which comprised the dose orientation test, preliminary test and actual phase test/LD50 determination, and the Thomson–Weil formula was applied to measure the lethal concentration 50 (LC50) of nanoherbal haramonting. Organs were collected for histological investigation after 14 days of the lethality test. Results: Nanoherbal haramonting had an average diameter distribution of 600.1 nm ± 135.8. It contained flavonoids, steroids, glycosides, saponins and tannins. Its LC50 and LD50 values were 2961.535 ppm and 10.4 ± 0.135 mg/kg BW, respectively. The histology of the heart, kidney, lungs, heart and brain were changed and affected by nanoherbal haramonting treatment at each dose level. Conclusion: Nanoherbal haramonting has strong antioxidative activity and small size, can be effectively used as medicine in the future because it contains secondary metabolite compounds that can be developed as drugs. However, it has mild toxicity.

Highlights The administration of nanoherbal andaliman reduced apoptosis via cytochrome c and FasL. EVOO reduces apoptosis via cytochrome c and FasL better than andaliman. Combination Nano herbal andaliman and EVOO reduce reduced apoptosis via cytochrome c and FasL on placental histology of hypertension rats. Combination Nano herbal andaliman and EVOO reduce MDA levels in hypertension rats. Nano herbal andaliman and combined with EVOO increase HSP-70 expression in hypertension rats.

Bangunbangun (Plectranthus amboinicus Lour) consumed by the mother, who just gave birth in North Sumatra, Indonesia in particular the Batak tribe, to increase the production of breast milk. This plant is known to have a high content of nutrients, especially iron and carotene. Also known to have many benefits, among others, as an antipyretic, analgesic, wound medicine, cough medicine, and thrush, antioxidant, antitumor, anticancer, and hypotensive. The study was conducted to determine levels of apigenin of Bangunbangun's leaves and evaluate its immunostimulatory activity in rats (Rattus norvegicus). Analysis of apigenin using High Performance Liquid Chramtography method (HPLC). Evaluation of immunostimulatory activity carried out by measuring the levels of imonoglobulin G (IgG), imonoglobulin M (IgM), Lysozyme and Monocytes. Analysis of IgG and IgM are using Elisa method (Sigma). Serum lysozyme activity was measured by the spectrophotometric method. Monocytes were analyzed by using ABX Micros 60. Organ histology preparations made by hematoxylin-eosin staining. Data were analyzed by ANOVA and showed that by giving the Ethanol Extract of Propolis (EEP) of 500 mg / kg bw in rats, with a significant increase of IgM and lysozyme activity with very significant. EEP give a very significant effect on levels of IgG. Monocytes were higher in mice given EEP, but did not differ significantly compared with mice not given EEP. Lymphoid organ weights are all under normal circumstances. Giving EEP 500 mg / kg bw mice, significantly increased the weight of the liver and spleen, but does not affect kidney weight.

BACKGROUND: Haramonting leaves have a large antioxidant activity as inhibitors oxidation and form non-free radical compounds that are not reactive and relatively stable. Electronic smoking is a choice who slowly want to quit conventional smoking.AIM: This research aimed to determine the effect of ethanol extract on hard mounting leaves on leukocyte counts and histology Pulmo on male mice exposed to an electronic cigarette.METHODS: The samples used in this study were healthy male mice aged 8-11 weeks and weight of 25-30 g as many as 25 individuals and ethanol extracts of hard mounting leaves with a dose of 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW. Blood tests were carried out at the Health Laboratory of North Sumatra Office, and the preparation of Pulmo histology was carried out at the Pathology and Anatomy Laboratory of Adam Malik General Hospital MedanRESULT: There were significant differences between the control group and all of the treatment groups in the number of lymphocytes, neutrophils, basophils and eosinophils. There was a tendency that electronic cigarette smoke could cause damage to pulmo tissue.CONCLUSION: The results of this study indicate that the ethanol extract of hard mounting leaves could help the immune system in mice exposed to an electronic cigarette.