The study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 after treatment of PAN. The percentage viability of the cell were carried out by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis was observed by flowcytometry assay in single dose of PAN. The expression of cyclin D1, Bcl2 and COX-2 proteins were investigated on HeLa cell lines by using immunocytochemistry. The result showed that PAN showed strong synergistic effect with doxorubicin againts HeLa cells based on Combination Index analysis. No significant apoptotic induction of PAN in HeLa cell lines, but the extract caused necrosis. The immunocytochemical study showed suppression of cyclin D1, Bcl2 and COX-2 expression on HeLa cell lines. The results concluded that PAN could be a potential cochemoterapeutic agent with doxorubicin on cervix cancer cells but need to be explored further by its combination on specific molecular target.

Introduction: Rhodomyrtus tomentosa (haramonting), a typical plant of North Sumatera, Indonesia, contains important medicinal ingredients. Nano sized drugs have high loading capacities and can be given at high concentrations. This study aimed to determine the components and toxicity of nanoherbal haramonting. It also aimed to determine the effect of nanoherbal haramonting on the histology of the liver, kidneys, lungs, heart and brain. Methods: High-energy milling was performed to produce nanoherbal haramonting. Thin-layer chromatography was utilised to determine the chemical components of the nanoherb. Antioxidant tests were performed by using the 1,1-diphenyl-2-picryhydrazil method. The three-stage of lethal dose 50 (LD50) which comprised the dose orientation test, preliminary test and actual phase test/LD50 determination, and the Thomson–Weil formula was applied to measure the lethal concentration 50 (LC50) of nanoherbal haramonting. Organs were collected for histological investigation after 14 days of the lethality test. Results: Nanoherbal haramonting had an average diameter distribution of 600.1 nm ± 135.8. It contained flavonoids, steroids, glycosides, saponins and tannins. Its LC50 and LD50 values were 2961.535 ppm and 10.4 ± 0.135 mg/kg BW, respectively. The histology of the heart, kidney, lungs, heart and brain were changed and affected by nanoherbal haramonting treatment at each dose level. Conclusion: Nanoherbal haramonting has strong antioxidative activity and small size, can be effectively used as medicine in the future because it contains secondary metabolite compounds that can be developed as drugs. However, it has mild toxicity.

Pekerjaan Sekretaris dalam kantor membutuhkan media teknologi informasi. Dengan menggunakan bantuan  teknologi  tersebut pekerjaan  kantor dapat efisien dan efektif. Hal tersebut menjadikan  kerja sekretaris lebih produktif.  Karir sekretaris dapat dibangun melalui peningkatakn ketrampilan  teknologi informasi maupun pengembangan soft skill. Perpaduan tersebut  akan lebih mengeksiskan peran sekretaris  dalam memberikan kontribusinya pada kemajuan pimpinan maupun  perusahaan. Dalam tulisan  ini akan dikupas hal-hal penting kemampuan teknologi informasi bagi sekretaris serta kiat mengembangkan karir sekretaris Kata kunci: Teknologi Informasi,  Sekretaris, Karir Sekretaris  Abstract: Role of Technology for Secretary Career Development. Secretary's work in the office requires information technology media. By using technology assistance, office work can be efficient and effective. This makes the work of the secretary more productive. Secretarial careers can be built through enhancing information technology skills and developing soft skills. This combination will further exert the role of secretary in contributing to the progress of the leadership and the company. In this paper, important information technology capabilities for secretaries and tips on developing a career for the secretary will be discussed. Keyword: Information Technology, Secretary, Career Secretary

Highlights The administration of nanoherbal andaliman reduced apoptosis via cytochrome c and FasL. EVOO reduces apoptosis via cytochrome c and FasL better than andaliman. Combination Nano herbal andaliman and EVOO reduce reduced apoptosis via cytochrome c and FasL on placental histology of hypertension rats. Combination Nano herbal andaliman and EVOO reduce MDA levels in hypertension rats. Nano herbal andaliman and combined with EVOO increase HSP-70 expression in hypertension rats.

Kendala dalam produksi benih ikan lele adalah derajat penetasan yang rendah karena sifat adhesif yang dimiliki oleh telur. Telur yang saling melekat menghambat masuknya oksigen pada telur sehingga dapat menghambat perkembangan telur dan akan berdampak terhadap daya tetas telur yang rendah. Salah satu cara untuk mengurangi daya rekat telur adalah dengan mencampurkan tanin kedalam telur ikan, salah satu bahan yang mengandung tanin adalah teh. Penelitian ini bertujuan untuk menentukan konsentrasi larutan teh yang efektif untuk menurunkan daya rekat telur dan pengaruhnya terhadap daya tetas telur ikan lele. Penelitian ini dilakukan pada tanggal 16 hingga 27 April 2020 di Balai Besar Perikanan Budidaya Air Tawar Kota Sukabumi. Metode penelitian yang digunakan adalah metode eksperimen dengan menggunakan Rancangan Acak Lengkap. Perlakuan yang digunakan adalah empat perlakuan konsentrasi larutan teh (0 g/L, 8 g/L, 10 g/L dan 12 g/L) dengan lama perendaman empat menit. Masing-masing perlakuan diulang sebanyak tiga kali. Wadah penelitian yang digunakan adalah akuarium dengan ukuran 40 x 60 x 40 cm3 sebanyak 12 buah dan menggunakan saringan yang diisi telur, pengamatan daya rekat dilakukan selama satu hari dan dilanjutkan dengan pengamatan derajat pembuahan dan derajat penetasan. Hasil penelitian menunjukan bahwa penggunaan larutan teh dengan konsentrasi 10 g/L selama empat menit efektif menurunkan daya rekat telur ikan lele dengan nilai 12% dan menghasilkan nilai daya tetas telur tinggi yaitu sebesar 63%. Hasil ini merupakan hasil uji statistik dengan uji jarak Berganda Duncan dengan taraf kepercayaan 95%, hasil yang di dapatkan berbeda nyata dengan kontrol.