Hematological Status in Neonatal Jaundice Patients and Its Relationship with G6PD Deficiency

Abstract

Background: The role of hemolysis in the pathophysiology of neonatal jaundice in patient with G6PD deficiency. Objectives: To observe the hematological status of hyperbilirubinemic neonates and its corelation with G6PD deficient neonates. Methods: The study was conducted on 90 male, term neonates with jaundice, age ranged from 3 to 12 days (Group B) in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU) between 1 st July 2007 to 30 th June 2008. On the basis of total serum bilirubin (TSB) level, study group was further divided into B 1 -TSB <15mg/ dl, B 2 -TSB 15 to 20mg/dl and B 3 -TSB>20mg/dl. For comparison age and sex matched 30 apparently healthy neonates (Group A) were also included in the study. Erythrocyte G6PD level was measured by Spectrophotometric method by using kit of Randox. Hematological parameters like hemoglobin concentration, hematocrit, total count of RBC, reticulocyte count were measured by standard laboratory technique. For statistical analysis anova, independent sample t test and Pearson's correlation coefficient test were performed as applicable by using SPSS for windows version-12. Results: In this study, hemoglobin concentration, hematocrit value and RBC count were significantly lower in both moderate (p<0.05) and severe (p<0.01) groups in comparison to those of control and also in mild group. However, these values were statistically not significant between study groups. Similarly, all these values were lower in G6PD deficient neonates than that of non deficient neonates but it was statistically non significant. Again, reticulocyte count was significantly (p<0.001) higher in severe hyperbilirubinemic than those of control, mild and moderate group. Hemoglobin concentration, hematocrit, total count of red blood cell and reticulocyte count showed non significant positive correlations (r=+.745, r=+.205, r=+.329, r=+.104) with erythrocyte G6PD level in deficient neonates. Conclusion: Hemolysis is one of the risk factor to develope hyperbilirubinemia in G6PD deficient neonates and degree of hemolysis depends on state of deficiency.

Glucose-6-Phosphate Dehydrogenase Status in the Term Newborns and Their Clinical and Other Laboratory Correlates

“…These findings support the postulation of the mechanism of neonatal jaundice in G6PD deficiency other than hemolysis. 29 In a study conducted in Bangladesh, Akter et al 30 showed that hemoglobin concentration, hematocrit, total count of red blood cell and reticulocyte count showed non-significant positive correlations (r=0.745, r=0.205, r=0.329, r=0.104) with erythrocyte G6PD level in deficient neonates. But in the present study, there was highly significant negative correlation between G6PD enzyme and hemoglobin levels (r= -0.314, p=0.001).…”

Glucose-6-Phosphate Dehydrogenase Deficiency and Malaria: A Method to Detect Primaquine-Induced Hemolysis in vitro

“…Wet, dry or frozen fava bean ingestion of grains, even if the mother eats fava beans can cause hemolysis in newborn infants through breast milk may occur (Luzzatto, 2001). Individuals with G6PD deficiency hemolytic effect caused by the beans contained many glycosides that are toxic due to the visin and konvisin (Beutler, 1994, Akhter, et al, 2011. In addition, β-glucosides in bean seeds, maturity stage of fava beans attain very high amounts causing a severe course of hemolytic crisis (Katz & Schall, 1979, Greene, 1993, Beutler, 1994.…”