Background: G6PD deficiency is one of the common inherited enzymatic disorder associated with high incidence of severe neonatal hyperbilirubinemia. Objectives: To observe G6PD status in male, term neonates with jaundice and its correlation with serum level of bilirubin. Methods: This cross sectional study was conducted on 90 male, term neonates with jaundice, age ranged from 3 to 12 days (Group B) in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU) between July 2007 to June 2008. On the basis of total serum bilirubin (TSB) level, study group was further divided into B 1 (TSB <15mg/dl), B 2 (TSB 15-20mg/dl) and B 3 (TSB>20mg/dl). For comparison age and sex matched 30 apparently healthy neonates (Group A) were also included in the study. Erythrocyte G6PD level was measured by Spectrophotometric method by using kit of Randox. Serum bilirubin level was measured by standard laboratory technique. For statistical analysis ANOVA, independent sample "t" test and Pearson's correlation coefficient test were performed as applicable by using SPSS windows version-12. Results: In this study, erythrocyte G6PD levels were significantly lower in moderate (p<0.01) and severe (p<0.001) hyperbilirubinemic group in comparison to that of control group . However, this enzyme level was lower in mild group compared to that of control but the difference was statistically non significant. Again, this enzyme levels were significantly lower in moderate (p<0.05) and severe (p<0.01) group than that of mild group and also between severe and moderate hyperbilirubinemic group (p<0.05). In this study, G6PD enzyme deficient were found in 1(3.33%) and 6(20%) subjects of group B 2 and B 3 respectively. Though, percentage of the subjects with enzyme deficiency were higher in severe group ( B 3 ) compared to that of moderate group( B 2 ) but the difference was statistically not significant. However, no enzyme deficient patient were found in control group (A) and mild hyperbilirubinemic group (B 1 ). Serum bilirubin level showed significant (p<0.05) positive (r=+.429) correlation with erythrocyte G6PD level in control group (A). On the other hand, this level was negatively correlated with G6PD enzyme in groups B 1 (r= -.127), B 2 (r=-.120) and B 3 ( r= -.671) but significant negative correlation in group B 3 (p<0.01). Conclusion: The results of the study revealed that severity of hyperbilirubinemia depends on degree of G6PD deficiency. Therefore, early detection of this enzymopathy and close surveillance of the affected neonates may be important in reducing the complications of severe hyperbilirubinemia.

Background: The role of hemolysis in the pathophysiology of neonatal jaundice in patient with G6PD deficiency. Objectives: To observe the hematological status of hyperbilirubinemic neonates and its corelation with G6PD deficient neonates. Methods: The study was conducted on 90 male, term neonates with jaundice, age ranged from 3 to 12 days (Group B) in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU) between 1 st July 2007 to 30 th June 2008. On the basis of total serum bilirubin (TSB) level, study group was further divided into B 1 -TSB <15mg/ dl, B 2 -TSB 15 to 20mg/dl and B 3 -TSB>20mg/dl. For comparison age and sex matched 30 apparently healthy neonates (Group A) were also included in the study. Erythrocyte G6PD level was measured by Spectrophotometric method by using kit of Randox. Hematological parameters like hemoglobin concentration, hematocrit, total count of RBC, reticulocyte count were measured by standard laboratory technique. For statistical analysis anova, independent sample t test and Pearson's correlation coefficient test were performed as applicable by using SPSS for windows version-12. Results: In this study, hemoglobin concentration, hematocrit value and RBC count were significantly lower in both moderate (p<0.05) and severe (p<0.01) groups in comparison to those of control and also in mild group. However, these values were statistically not significant between study groups. Similarly, all these values were lower in G6PD deficient neonates than that of non deficient neonates but it was statistically non significant. Again, reticulocyte count was significantly (p<0.001) higher in severe hyperbilirubinemic than those of control, mild and moderate group. Hemoglobin concentration, hematocrit, total count of red blood cell and reticulocyte count showed non significant positive correlations (r=+.745, r=+.205, r=+.329, r=+.104) with erythrocyte G6PD level in deficient neonates. Conclusion: Hemolysis is one of the risk factor to develope hyperbilirubinemia in G6PD deficient neonates and degree of hemolysis depends on state of deficiency.

Background: Neonatal jaundice is a common cause of newborn hospital admission. G6PD enzyme defects is one of the factor to develop neonatal hyperbilirubinemia, which may be related to abnormal liver function in this group of neonates. Objective: To observe some aspects of liver function status by measuring serum bilirubin and alanine amino transferase levels in G6PD deficient neonates and also their relationship with erythrocyte G6PD levels. Methods: The study was conducted on 30 male, term neonates with G6PD deficiency in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU) between 1st July 2007 to 30th June 2008. For comparison age and sex matched 30 apparently healthy neonates without deficiency (control group) were also included in the study. Erythrocyte G6PD level was measured by Spectrophotometric method by using kit of Randox. Alanine amino transferase level was done by Schumann and Klauke method. For statistical analysis independent sample "t" test and Pearson's correlation coefficient test were performed as applicable by using SPSS for windows version-13 Results: In this study, significantly (p<0.001)higher level of serum bilirubin & ALT were found in G6PD deficient group in comparison to those of non-deficient group. Further more, serum bilirubin & ALT levels were negatively (r=-.671, (r= -.534) correlated with erythrocyte G6PD level and it was statistically significant (p<0.01) in G6PD deficient group. Again, significant (p<0.05) positive (r=+.429) correlation of serum bilirubin level was observed in non deficient group. Whereas, non significant positive (r=+.041) correlations of ALT was found in non deficient group. Conclusion: From the study results it can be concluded that higher level of alanine amino transferaseand hyperbilirubinemia were present in G6PD deficient neonates & increase serum bilirubin and serum alanine amino transferase levels were associated with degree of G6PD enzyme deficiency.

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The present study was designed to observe ABC and haemoglobin concentration in female table/es. For this purpose, a total member of 60 /oaks. age ranged from 17-38 years. were selected Thirty of them were athletes 101IperillK011eli group) and 30 non-athletes (comrolh Athletes were selected form Dhanonadi Mohan Krira Comptes ad controls fiat students of Dhaka University. ROC man and haemoglobin concentration were measured by electronk reO water method. RBC moot was land significantly lower in athletes than that of controls but Mere was no change in haemoglobin concentration. The reat of the study 'weals that lowered RBC coma was due to mechanical damage of ersahrocytic membrane leading to shortening of mean life span of ROC.