Hematology and serum biochemistry reference ranges of horses of different breeds and age measured with newest clinicopathological methods

Köller, G.; Gieseler, T.; Schusser, G. F.

doi:10.21836/pem20140402

Cited by 19 publications

(10 citation statements)

References 10 publications

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“…The first available blood sample from the second day of disease showed marked increases in creatinine kinase (CK: 58,205 u/l; normal CK activity in Warmblood type horses: 146–346 u/l, Koeller et al . ) and lactate dehydrogenase (LDH: 53,990 u/l; normal LDH activity in Warmblood type horses: 293–822 u/l, Koeller et al . ).…”

Section: Resultsmentioning

confidence: 99%

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Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners

Bochnia

Scheidemann²,

Ziegler

et al. 2016

Equine Veterinary Education

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Summary Hypoglycin A (HGA) was detected in blood and urine of a horse suffering from atypical myopathy (AM; Day 2, serum, 8290 μg/l; urine: Day 1, 574, Day 2, 742 μg/l) and in its cograzing partners with a high variability (46–1570 μg/l serum). Over the period of disease, the level of the toxic metabolites (methylencyclopropylacetic acid [MCPA]‐conjugates) increased in body fluids of the AM horse (MCPA‐carnitine: Day 2, 0.246, Day 3, 0.581 μmol/l serum; MCPA‐carnitine: Day 2, 0.621, Day 3, 0.884 μmol/mmol creatinine in urine) and HGA decreased rapidly (Day 3, 2430 μg/l serum). In cograzing horses MCPA‐conjugates were not detected. HGA in seeds ranged from 268 to 367 μg/g. Although HGA was present in body fluids of healthy cograzing horses, MCPA‐conjugates were not detectable, in contrast to the AM horse. Therefore, increasing concentrations of MCPA‐conjugates are supposed to be linked with the onset of AM and both parameters seem to indicate the clinical stage of disease. However, detection of HGA in body fluids of cograzing horses might be a promising step in preventing the disease.

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Section: Resultsmentioning

confidence: 99%

“…) and lactate dehydrogenase (LDH: 53,990 u/l; normal LDH activity in Warmblood type horses: 293–822 u/l, Koeller et al . ). The colour of the urine was reddish or nearly brownish.…”

Section: Resultsmentioning

confidence: 99%

Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners

Bochnia

Scheidemann²,

Ziegler

et al. 2016

Equine Veterinary Education

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“…After giving the horse intensive care (treatment included intravenous administration of fluids 70–100 mL/kg bwt/day, flunixin-meglumin over 7 days) a paralysis of the tongue and a slightly lameness was observed, but this was reversible after 4–6 weeks. Available blood samples from all horses showed a marked increase in CK (range 6,154–1,215,987 U/L, normal: Warmblood 146–346 U/L, Haflinger and Pony 181–652 U/L [ 35 ]) and LDH (range 816–43,802 U/L, normal: Warmblood 223–536 U/L, Haflinger and Pony 293–822 U/L [ 35 ]).…”

Section: Resultsmentioning

confidence: 99%

Hypoglycin A Content in Blood and Urine Discriminates Horses with Atypical Myopathy from Clinically Normal Horses Grazing on the Same Pasture

Bochnia

Ziegler

Sander³

et al. 2015

PLoS ONE

100

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Hypoglycin A (HGA) in seeds of Acer spp. is suspected to cause seasonal pasture myopathy in North America and equine atypical myopathy (AM) in Europe, fatal diseases in horses on pasture. In previous studies, this suspicion was substantiated by the correlation of seed HGA content with the concentrations of toxic metabolites in urine and serum (MCPA-conjugates) of affected horses. However, seed sampling was conducted after rather than during an outbreak of the disease. The aim of this study was to further confirm the causality between HGA occurrence and disease outbreak by seed sampling during an outbreak and the determination of i) HGA in seeds and of ii) HGA and MCPA-conjugates in urine and serum of diseased horses. Furthermore, cograzing healthy horses, which were present on AM affected pastures, were also investigated. AM-pastures in Germany were visited to identify seeds of Acer pseudoplatanus and serum (n = 8) as well as urine (n = 6) from a total of 16 diseased horses were analyzed for amino acid composition by LC-ESI-MS/MS, with a special focus on the content of HGA. Additionally, the content of its toxic metabolite was measured in its conjugated form in body fluids (UPLC-MS/MS). The seeds contained 1.7–319.8 μg HGA/g seed. The content of HGA in serum of affected horses ranged from 387.8–8493.8 μg/L (controls < 10 μg/L), and in urine from 143.8–926.4 μg/L (controls < 10 μg/L), respectively. Healthy cograzing horses on AM-pastures showed higher serum (108.8 ± 83.76 μg/L) and urine concentrations (26.9 ± 7.39 μg/L) compared to control horses, but lower concentrations compared to diseased horses. The range of MCPA-carnitine and creatinine concentrations found in diseased horses in serum and urine were 0.17–0.65 mmol/L (controls < 0.01), and 0.34–2.05 μmol/mmoL (controls < 0.001), respectively. MCPA-glycine levels in urine of cograzing horses were higher compared to controls. Thus, the causal link between HGA intoxication and disease outbreak could be further substantiated, and the early detection of HGA in cograzing horses, which are clinically normal, might be a promising step in prophylaxis.

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“…Laboratory examination revealed leukocytosis (15.3 G/l; normal range: 4.4 -12 G/l), characterised by segmented neutrophilia (12.85 G/l; normal range: 2 -6.9 G/l). Only the creatine kinase activity was elevated at 564 U/l (normal range 146 -354 U/l) in clinicopathological tests (Köller et al 2014). Serum electrophoresis showed normal protein concentrations.…”

Section: Casementioning

confidence: 91%

Cervical Vertebral Compressive Myelopathy associated with articular processes osteoarthritis in three horses

Adam¹,

Arnold²,

Ehlers³

et al. 2016

PHK

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Summary: Cervical vertebral compressive myelopathy is a neuropathy resulting in ataxia in horses. Three horses with different signs of incoordination were presented for further evaluation. A five-year-old gelding with a long history of abnormal head and neck carriage, a six-yearold gelding with a history of previous neurological episodes and a two-day history of acute episodes of walking difficulties, and a sixteenyear-old mare with a one-month history of left fore-limb ataxia were diagnosed with osteoarthritis of the facet joints of the caudal cervical vertebrae. Lateral radiographs of caudal cervical vertebrae confirmed osteoarthritis of the articular processes. Radiographic changes, such as enlargement of the articular process joint, periarticular new bone and reduction or narrowing of the intervertebral foramen, were observed in all three cases. Cervical vertebral compressive myelopathy should be considered in ataxic horses with normal laboratory findings.

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Hematology and serum biochemistry reference ranges of horses of different breeds and age measured with newest clinicopathological methods

Cited by 19 publications

References 10 publications

Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners

Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners

Hypoglycin A Content in Blood and Urine Discriminates Horses with Atypical Myopathy from Clinically Normal Horses Grazing on the Same Pasture

Cervical Vertebral Compressive Myelopathy associated with articular processes osteoarthritis in three horses

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