Hematoporphyrin derivative‐mediated photodynamic therapy inhibits tumor growth in human cholangiocarcinoma <i>in vitro</i> and <i>in vivo</i>

Cao, Liangqi; Xue, Ping; Lu, Hai-wu; Zheng, Qiang; Wen, Zilong; Shao, Zhijian

doi:10.1111/j.1872-034x.2009.00569.x

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…Cao et al reported that hematoporphyrin, a photosensitizer similar to ALA, exhibits a PDT-mediated cytotoxic effect against human cholangiocarcinoma cells both in vitro and in vivo. 32 The results of the authors' study show that ALA-based PDT is no less effective for treatment of human cholangiocarcinoma than hematoporphyrin.…”

Section: Dovepressmentioning

confidence: 80%

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Kim

Chung²,

Choi³

et al. 2011

IJN

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Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05–2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.

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Section: Dovepressmentioning

confidence: 80%

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Kim

Chung²,

Choi³

et al. 2011

IJN

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“…“Resistive-miRs” 20a*, 141, 200a, 200c and 203 revealed targets involved in ROS detoxification (e.g., HMOX1 indirectly via BACH1, [ 9 , 73 , 74 , 75 , 76 , 77 ]) adaptation to ROS stress (e.g., NOX1, [ 78 ]), apoptotic signaling (e.g., BAX, [ 79 , 80 , 81 ]) and survival mechanisms (e.g., KEAP1, [ 82 ]). Their targets are enriched in GO terms “apoptotic mitochondrial changes”, “release of cytochrome c from mitochondria”, “positive regulation of MAPKKK cascade”, “regulation of JNK cascade” and “regulation of stress-activated protein kinase signaling pathway”.…”

Section: Resultsmentioning

confidence: 99%

MicroRNAs Associated with the Efficacy of Photodynamic Therapy in Biliary Tract Cancer Cell Lines

Wagner

Mayr

Bach

et al. 2014

IJMS

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Photodynamic therapy (PDT) is a palliative treatment option for unresectable hilar biliary tract cancer (BTC) showing a considerable benefit for survival and quality of life with few side effects. Currently, factors determining the cellular response of BTC cells towards PDT are unknown. Due to their multifaceted nature, microRNAs (miRs) are a promising analyte to investigate the cellular mechanisms following PDT. For two photosensitizers, Photofrin® and Foscan®, the phototoxicity was investigated in eight BTC cell lines. Each cell line (untreated) was profiled for expression of n = 754 miRs using TaqMan® Array Human MicroRNA Cards. Statistical analysis and bioinformatic tools were used to identify miRs associated with PDT efficiency and their putative targets, respectively. Twenty miRs correlated significantly with either high or low PDT efficiency. PDT was particularly effective in cells with high levels of clustered miRs 25-93*-106b and (in case of miR-106b) a phenotype characterized by high expression of the mesenchymal marker vimentin and high proliferation (cyclinD1 and Ki67 expression). Insensitivity towards PDT was associated with high miR-200 family expression and (for miR-cluster 200a/b-429) expression of differentiation markers Ck19 and Ck8/18. Predicted and validated downstream targets indicate plausible involvement of miRs 20a*, 25, 93*, 130a, 141, 200a, 200c and 203 in response mechanisms to PDT, suggesting that targeting these miRs could improve susceptibility to PDT in insensitive cell lines. Taken together, the miRNome pattern may provide a novel tool for predicting the efficiency of PDT and—following appropriate functional verification—may subsequently allow for optimization of the PDT protocol.

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“…The 46 NPC patients received HPD during the course of radiotherapy to enhance radiosensitivity. However, recent studies have shown that HPD can’t function as a radiosensitizer for the radiation treatment of cancer [ 15 , 16 ]. Therefore, it cannot affect the efficacy of radiotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…Under the background of researches which were conducted 20 years ago, HPD was assumed to be a radiosensitizer and can enhance the sensitivity of some types of cancer cells to radiation [ 13 , 14 ]. However, after more than 20 years of experience using HPD to treat tumors, recent studies showed that HPD was the most frequently used photosensitizer in photodynamic therapy (PDT) [ 15 , 16 ]. This therapy is based on the administration of a photosensitizer followed by localized exposure of the target tissue to laser-generated light.…”

Section: Introductionmentioning

confidence: 99%

Forty-six cases of nasopharyngeal carcinoma treated with 50 Gy radiotherapy plus hematoporphyrin derivative: 20 years of follow-up and outcomes from the Sun Yat-sen University Cancer Center

Xu¹,

Tu²,

Tao³

et al. 2016

Chin J Cancer

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BackgroundWith the improved overall survival (OS) of nasopharyngeal carcinoma (NPC) patients, the importance of quality of life (QoL) is increasingly being recognized. For some radiosensitive NPC patients, whether low-dose radiotherapy can improve the QoL without affecting clinical efficacy is unknown. This study aimed to assess the survival rates and QoL of NPC patients treated with 50 Gy radiotherapy plus hematoporphyrin derivative (HPD).MethodsForty-six newly diagnosed NPC patients treated with 50 Gy radiotherapy plus HPD between June 1988 and July 1992 were analyzed. All patients were restaged according to the 7th edition of the American Joint Committee on Cancer staging system. The radiotherapy plan was designed on the basis of pretreatment computed tomography. The OS, local recurrence-free survival (LRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) rates were estimated using the Kaplan–Meier method. QoL was assessed using the Late Radiation Morbidity Scoring Criteria of the Radiation Therapy Oncology Group.ResultsThe 5-year OS, LRFS, DMFS, and DFS rates were 74.3%, 72.6%, 82.1%, and 61.2%, respectively. The corresponding 10-year rates were 38.4%, 62.9%, 78.5%, and 49.8%, respectively, and the 20-year rates were 27.7%, 51.4%, 78.5%, and 40.7%, respectively. None of the patients developed severe radiation-related complications, such as radiation-induced temporal lobe necrosis, hearing loss, trismus, and dysphagia.ConclusionSome NPC patients were sensitive to 50 Gy radiotherapy plus HPD, and this sensitivity was characterized by long-term survival without significant late treatment morbidities.

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Hematoporphyrin derivative‐mediated photodynamic therapy inhibits tumor growth in human cholangiocarcinoma in vitro and in vivo

Abstract: HPD-PDT inhibits tumor growth of human cholangiocarcinoma, suggesting that HPD-PDT is useful in cholangiocarcinoma therapy.

Cited by 11 publications

References 28 publications

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

MicroRNAs Associated with the Efficacy of Photodynamic Therapy in Biliary Tract Cancer Cell Lines

Forty-six cases of nasopharyngeal carcinoma treated with 50 Gy radiotherapy plus hematoporphyrin derivative: 20 years of follow-up and outcomes from the Sun Yat-sen University Cancer Center

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