Hematoxylin and Eosin Staining of Tissue and Cell Sections

Fischer, Andrew H.; Jacobson, Kenneth A.; Rose, Jack; Zeller, Rolf

doi:10.1101/pdb.prot4986

Cited by 1,470 publications

(1,075 citation statements)

References 26 publications

(29 reference statements)

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“…Coronal sections of PFC and hippocampus were obtained according to stereotaxic coordinates (+4 mm) from each brain. Histological staining was carried out in paraffin wax embedded sections which were stained with Haematoxylin and Eosin (H&E) using the methods described by Fischer et al [9] Histochemical demonstration of Nissl substances was done with slight modification to the method published by Kádár et al [10] Determination of SOD, LDH and MDA activities was carried out on whole brains of treated rats using spectrophotometric technique. Each of the assay kits were procured from Bio Legend Inc., San Diego, CA, USA.…”

Section: Methodsmentioning

confidence: 99%

Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Arogundade¹,

Enaibe²,

Adigun³

et al. 2017

Anatomy

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Objectives: Mustard seeds, apart from being a culinary essential, have had medicinal applications dating back to the time of Hippocrates. It has in fact been once mentioned as the greatest herb ever. We explored the dose dependent effects of the crude aqueous extract of Brassica nigra (Black mustard seeds) on the prefrontal cortex of adult Wistar rats.Methods: 20 adult female rats weighing an average of 180±20 g were used. They were split into 4 groups (n=5); Group A (received extract at 200 mg/kg body weight), Group B (received extract at 100 mg/kg body weight), Group C (received extract at 50 mg/kg body weight), and Group D (received distilled water ad libitum). All of the animals were subjected to the Y-maze spontaneous alternation test for neurobehavioural analyses following 28-day administration of the extracts. Animals were sacrificed 24 hours after taking the last day of administration. Results:Our results showed that neurobehavioural analyses are significantly hampered in animals receiving 200 mg/kg extract in comparison to the control group. In treatment groups, increased dose of extract elevated the level of MDA, but reduced the level of SOD. LDH levels were also significantly increased in the 200 mg/kg treated group when comparing with the control. General microarchitecture in the prefrontal cortex of 200 mg/kg Brassica nigra treated group showed signs of karyolysis and pyknosis. Conclusion: Brassica nigra (black mustard) is not innocuous and therefore it should be consumed in moderation.Keywords: Brassica nigra; neurobehaviour; neurototoxicity; prefrontal cortex Original Article www.anatomy.org.tr Received: August 13, 2016; Accepted: September 2, 2017 doi:10.2399 the cell-poor molecular layer and the deepest is the multiform (polymorphic) layer, which is populated largely by fusiform cells. Between these two layers, there are four layers that are alternatively mostly populated by stellate or pyramidal cells. The executive functions of the frontal cortex involve the ability to recognize future consequences resulting from current actions, to choose between good and bad actions (or better and best), override and suppress unacceptable social responses, and determine similarities and differences between things or events. [7] Therefore, it is involved in higher mental functions. The frontal cortex also plays an important role in retaining long-term memories which are not task-based. These are memories associated often with emotions derived input from the limbic system. The frontal cortex modifies those emotions to generally fit socially acceptable norms.[7] Thus, we specifically examined the dose-dependent effects of aqueous Brassica nigra seed extracts on the cognitive and memory functions, oxidative parameters, and finally, the histology of the PFC in Wistar rats. We examined the oxidative damage by assessing the level of superoxide dismutase (SOD), malondialdehyde (MDA) and lactate dehydrogenase (LDH) enzymes. Materials and MethodsAll protocols and treatment procedures were done according to t...

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Section: Methodsmentioning

confidence: 99%

Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Arogundade¹,

Enaibe²,

Adigun³

et al. 2017

Anatomy

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“…One of the infected livers was processed and stained with hematoxylin and eosin based on a prior study 19,20 . Briefly, one part of the liver was trimmed, fixed in 10% formaldehyde for 24 hours, dehydrated in each of the 50%, 70%, 90% and 2 series of 100% ethanol for 15 minutes.…”

Section: Sectioning and Stainingmentioning

confidence: 99%

Capillaria hepatica in Rattus Spp. Captured in Sanandaj and Orally uninfected Balb/C Mice With Embryonated Eggs

Zamini¹,

Khadem-Erfan²,

Faridi³

2017

Int J Basic Sci Med

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“…Tissue sections were prepared with 3 µm thickness using microtome device and the staining procedure was performed as described before. 9 IHC: IHC for p16 INK4a was performed on all of the samples (100 samples). The procedure of IHC has performed according to the manuals of antibody detection systems.…”

Section: Hematoxylin and Eosin Staining (Hande)mentioning

confidence: 99%

Survey of p16INK4a immunohistochemistry in diagnosis of dysplastic changes in cervix

Esmaieli¹,

Berenjian²

2017

J Anal Res Clin Med

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CervixCervical cancer is the third most common cancer and the second most frequent cause of mortality from malignancies of genital organs in women, which can be prevented by diagnosis of pre-neoplastic changes in cervix. This study aimed to evaluate the p16 INK4abiomarker in different grades of cervical intraepithelial neoplasia (CIN) using immunohistochemistry (IHC) method.The present cross-sectional study was carried out on the paraffin-embedded blocks of cervical tissue of 100 women with previous histopathological diagnosis of CIN referred to AlZahra Hospital, Tabriz, Iran, during 2015-2016. The samples were divided into 4 groups, 31 with normal cervical finding, 30 with low-grade CIN (CIN I), and 39 with high-grade CIN (16 CIN II and 23 CIN III). p16INK4a was investigated on the samples using IHC technique. Data was analyzed by SPSS using chi-square and Mann-Whitney U tests.Thirteen out of 30 (43%), 12 out of 16 (75%) and 23 out of 23 (100%) of the CIN I, CIN II, CIN III were positive for p16 INK4a , respectively. None of the normal samples were positive for p16 INK4a . Sensitivity of p16INK4a for detection of CIN I, CIN II, CIN III was calculated as 63%, 80% and 100%, respectively. The overall sensitivity of the biomarker for detection of CIN lesions was 76.6% and the specificity was 100% for all CIN grades.The p16 INK4A biomarker is a suitable diagnostic tool for high-grade CIN, yet for low-grade ones it has lower sensitivity. p16INK4a can be a helpful tool beside histopathology for diagnosis of CIN lesions.Citation: Esmaieli HA, Berenjian S. Survey of p16INK4a immunohistochemistry in diagnosis of dysplastic changes in cervix.

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Hematoxylin and Eosin Staining of Tissue and Cell Sections

Cited by 1,470 publications

References 26 publications

Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Capillaria hepatica in Rattus Spp. Captured in Sanandaj and Orally uninfected Balb/C Mice With Embryonated Eggs

Survey of p16INK4a immunohistochemistry in diagnosis of dysplastic changes in cervix

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