Hemin-decreased cadmium uptake in pak choi (Brassica chinensis L.) seedlings is heme oxygenase-1 dependent and relies on its by-products ferrous iron and carbon monoxide

Su, Nana; Niu, Mengyang; Liu, Ze; Wang, Lu; Zhu, Zhengbo; Zou, Jianwen; Chen, Yahua; Chen, Jin

doi:10.1016/j.envpol.2020.115882

Cited by 14 publications

(6 citation statements)

References 80 publications

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“…The biological effects of hemin application have been largely attributed to its induction of HO-1 in vivo [ 27 , 28 ]. To test if the role of hemin in anthocyanin accumulation was related to NO production, Zinc (II) Protoporphyrin IX (ZnPP) was used, due to its specific role in HO-1 activity inhibition.…”

Section: Resultsmentioning

confidence: 99%

Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO

Liu

Wang

et al. 2022

BMC Plant Biol

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Background The health benefits of anthocyanins impel researchers and food producers to explorer new methods to increase anthocyanin contents in plant foods. Our previous studies revealed a positive role of nitric oxide (NO) in anthocyanin accumulation in radish (Raphanus sativus L.) sprouts. The application of hemin, an inducer of heme oxygenase-1 (HO-1), can effectively elevate NO production in vivo. Hemin treatment also improves plant growth and stress tolerance. This study is aimed to assess the effects of hemin treatment on anthocyanin production in radish sprouts, and to investigate whether NO signalling is involved in this process. Results The application of hemin significantly up regulated the expressions of many anthocyanins biosynthesis related structure and regulatory genes, leading to increased anthocyanins accumulation in radish hypocotyls. Hemin treatment also raised NO contents in radish sprouts, probably through enhancing nitrate reductase (NR) activity and Nitric Oxide-Associated 1 (NOA1) expression. Comparing the effects of Zinc Protoporphyrin (ZnPP, HO-1 activity inhibitor), Sodium Nitroprusside (SNP, NO donor) and carboxy-PTIO (cPTIO, NO-scavenger) on anthocyanin and NO production, a positive role of NO signalling has been revealed in hemin-derived anthocyanin accumulation. A positive feedback loop between HO-1 and NO may be involved in regulating this process. Conclusions Hemin induced anthocyanin accumulation in radish sprouts through HO-1 and NO signalling network.

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Section: Resultsmentioning

confidence: 99%

Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO

Liu

Wang

et al. 2022

BMC Plant Biol

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“…After 1 week, seedlings successfully adapted to hydroponic culture were treated with exogenous hemin at concentrations ranging from 0.1 to 20 mM (0.1, 0.5, 1.0, 5.0, 10, 20 mM) for 96 h [53][54][55][56][57] . Liquid medium without treatment was considered a control and utilized to evaluate the effect of hemin on haem oxygenase-1 catalytic activity.…”

Section: Methodsmentioning

confidence: 99%

Exogenous hemin improves Cd2+ tolerance and remediation potential in Vigna radiata by intensifying the HO-1 mediated antioxidant defence system

Mahawar

Popek

Shekhawat

et al. 2021

Sci Rep

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The present study evaluated the effects of exogenous hemin on cadmium toxicity in terms of metal accretion and stress resilience in Vigna radiata L. (Wilczek). One-week-old seedlings were treated with CdCl2 (50 μM) alone and in combination with hemin (0.5 mM) in half-strength Hoagland medium for 96 h. The optimum concentrations of Cd and hemin were determined on the basis of haem oxygenase-1 activity. The results demonstrated that under Cd stress, plants accumulated a considerable amount of metal in their tissues, and the accumulation was higher in roots than in leaves, which significantly reduced the plant biomass and chlorophyll content by increasing the oxidative stress (MDA and H2O2 content). However, hemin supplementation under Cd,-stress improved plant growth by enhancing the harvestable biomass and photosynthetic pigments, increasing antioxidant activities (SOD, APX, POD, HO-1 and proline), lowering oxidative damage and increasing Cd tolerance in plants. Furthermore, the application of hemin enhances the removal efficiency of Cd in V. radiata by increasing the uptake of Cd via roots and its translocation from roots to foliar tissues. Thus, the study suggests that hemin has the potential to improve the stress tolerance and phytoremediation ability of heavy metal-tolerant plants so that they can be used instead of hyperaccumulators for remediation of Cd-contaminated environments.

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“…Hemin boosts the growth of the lateral roots of tomato 31 , promotes the growth of cucumber roots 32 , and increases the anthocyanin content of radish 33 . Research has proven that Hemin as a highly effective anti-stress agent is effective in alleviating abiotic stresses, such as heavy metal stress 34 , low-temperature stress 30 , and salt stress 35 . The spraying of Hemin on maize increased the chlorophyll content and net photosynthetic rate, delayed leaf senescence, and improved the accumulation and distribution of photosynthetic products.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Hemin alleviates NaCl stress by promoting photosynthesis and carbon metabolism in rice seedlings

Meng

Feng

Zheng

et al. 2023

Sci Rep

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It is widely known that salt stress restricts rice growth and productivity severely. However, little information is available regarding the stage of rice seedlings subjected to the Heme oxygenase 1 (HO-1) inducer, Hemin. This study aimed to investigate the effects of salt stress on two rice varieties (Huanghuazhan and Xiangliangyou 900) and the effect of Hemin in promoting photosynthesis, carbohydrate metabolism, and key enzymes under salt-stress conditions. At the stage of three leaves and one heart, Huanghuazhan (HHZ) and Xiangliangyou 900 (XLY900) were sprayed with 5 μmol·L−1 Hemin and then subjected to 50 mM NaCl stress. The results showed that NaCl stress decreased the contents of chlorophyll a, chlorophyll b, and carotenoids. Furthermore, the net photosynthetic rate (Pn) decreased remarkably and the starch content was also lowered. However, NaCl treatment enhanced the concentration of sucrose and soluble sugar, simultaneously enhancing the sucrose metabolism. Nevertheless, the foliar spraying of exogenous Hemin mediated the increase in fructose and starch content, along with the activities of key enzymes’ soluble acid invertase (SAInv), basic/neutral invertase (A/N-Inv), and sucrose synthase (SS) in rice leaves under NaCl stress. The sucrose phosphate synthase (SPS) in leaves decreased significantly, and the fructose accumulation in leaves increased. Hemin also mediated the increase of starch content and the α-amylase, total amylase, and starch phosphorylase (SP) activities under NaCl stress. Under stress conditions, the application of the Heme oxygenase 1 (HO-1) inhibitor, ZnPP failed to alleviate the damage to rice seedlings by NaCl stress. The ZnPP treatment showed similar tendency to the NaCl treatment on pigment content, gas exchange parameters and carbon metabolism related products and enzymes. However, ZnPP decreased carotenoids, fructose, starch content and enzyme activities related to starch metabolism. The regulation effect of Hemin on HuangHuaZhan was better than XiangLiangYou 900. These results indicate that Hemin improved the effects of salt stress on the photosynthesis and physiological characteristics of rice leaves as a result of enhanced carbohydrate metabolism. Thus, Hemin could alleviate the damage caused by salt stress to a certain extent.

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Hemin-decreased cadmium uptake in pak choi (Brassica chinensis L.) seedlings is heme oxygenase-1 dependent and relies on its by-products ferrous iron and carbon monoxide

Cited by 14 publications

References 80 publications

Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO

Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO

Exogenous hemin improves Cd2+ tolerance and remediation potential in Vigna radiata by intensifying the HO-1 mediated antioxidant defence system

Exogenous Hemin alleviates NaCl stress by promoting photosynthesis and carbon metabolism in rice seedlings

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