Hemin-incorporated nanoflowers as enzyme mimics for colorimetric detection of foodborne pathogenic bacteria

Wang, Kuiyu; Bu, Shengjun; Ju, Chuanjing; Li, Changtian; Li, Zhongyi; Han, Ye; Ma, Cambridge; Wang, Chengyu; Hao, Zhuo; Liu, Wensen; Wan, Jiayu

doi:10.1016/j.bmcl.2018.07.017

Cited by 43 publications

(20 citation statements)

References 34 publications

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“…Wang et al. () recently reported a colorimetric sandwich‐type detection of foodborne pathogens based on the direct adsorption of bacterial cells onto POD‐like hemin‐concanavalin A hybrid nanoflowers and the separation by antibody‐decorated magnetic beads. Mumtaz et al.…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

“…In addition to ELISA, there is another powerful immunodetection technique called lateral flow immunoassay (LFIA), which is a point-of-care bioassay performing on paper-based device . Typically, a LFIA strip consists of a sample pad for sampling liquid samples, a conjugation pad loaded with dark-colored NPs labeled with detection antibodies (Ab-NPs) (Bu et al, 2018;Wang, Yao et al, 2019;Yao et al, 2019), a test pad composed of test line (T-line) and control line (C line), and an absorbent pad (panel [a] of Figure 3B). In details, the target analytes are first dropped onto the sample pad ( Figure 3B, ai), and flow with the sample liquid by the capillary force to the conjugation pad, where the analyte-Ab-NP complexes are formed through binding of target analytes to the Ab-NPs ( Figure 3B, aii).…”

Section: Nanozyme As a Signal Tagmentioning

confidence: 99%

“…The visual signal of LFIA is mainly derived from the gathering effect of intrinsically colored NPs, such as AuNPs (Bu et al, 2018), which will fail if the concentration of NPs is too low to be visible. Therefore, probe materials are of significance to enhance the detection efficiency and sensitivity of LFIA (Bu, Huang, Sung et al, 2019, Bu, Wang et al, 2019Dou et al, 2019).…”

Section: Nanozyme As a Signal Tagmentioning

confidence: 99%

“…Similarly, Wen, Jing, Liang, and Jiang (2018) described a SERS detection strategy based on the controlled formation of SERS-active AuNPs adjusted by the POD-like GO nanoribbon-labeled immunoreaction. In addition, Bu et al (2018) presented an efficient approach for signal amplification in an immunochromatographic assay of Salmonella enteritidis (S. enteritidis) by means of the self-catalyzed growth of AuNPs. Liu, Wang, Song et al (2018) discovered that aggregation of AuNPs could be mediated by a typical catalytic substrate (OPD), which was applied in a rapid colorimetric assay for L. monocytogenes by use of AuNPs as output signal and silver NCs as OXD mimics.…”

Section: Nanozyme As a Signal Amplifiermentioning

confidence: 99%

See 3 more Smart Citations

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

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The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

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Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

Section: Nanozyme As a Signal Tagmentioning

confidence: 99%

Section: Nanozyme As a Signal Tagmentioning

confidence: 99%

Section: Nanozyme As a Signal Amplifiermentioning

confidence: 99%

See 2 more Smart Citations

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

View full text Add to dashboard Cite

show abstract

“…Such materials and composites find their application in designing biosensors towards various analytes ranging from small molecules to the whole cells with the working principle based on promotion or inhibition of catalytic activities [20][21][22]. This principle can also be used for bacteria detection, and modern trends are aimed at increasing the effectiveness and sensitivity of detection systems and at simplifying analysis processes, thus moving to the point of care diagnostics [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles

et al. 2020

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Rapid detection of bacterial contamination is an essential task in numerous medical and technical processes and one of the most rapidly developing areas of nano-based analytics. Here, we present a simple-to-use and special-equipment-free test-system for bacteria detection based on magnetite nanoparticle arrays. The system is based on peroxide oxidation of chromogenic substrate catalyzed by magnetite nanoparticles, and the process undergoes computer-aided visual analysis. The nanoparticles used had a pristine surface free of adsorbed molecules and demonstrated high catalytic activities up to 6585 U/mg. The catalytic process showed the Michaelis–Menten kinetic with Km valued 1.22 mmol/L and Vmax of 4.39 µmol/s. The nanoparticles synthesized were used for the creation of inkjet printing inks and the design of sensor arrays by soft lithography. The printed sensors require no special equipment for data reading and showed a linear response for the detection of model bacteria in the range of 104–108 colony-forming units (CFU) per milliliter with the detection limit of 3.2 × 103 CFU/mL.

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Nanoflowers: A New Approach of Enzyme Immobilization

Costa

Cipolatti

Fúrigo

et al. 2022

The Chemical Record

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Enzymes are biocatalysts known for versatility, selectivity, and brand operating conditions compared to chemical catalysts. However, there are limitations to their large-scale application, such as the high costs of enzymes and their low stability under extreme reaction conditions. Immobilization techniques can efficiently solve these problems; nevertheless, most current methods lead to a significant loss of enzymatic activity and require several steps of activation and functionalization of the supports. In this context, a new form of immobilization has been studied: forming organic-inorganic hybrids between metal phosphates as inorganic parts and enzymes as organic parts. Compared to traditional immobilization methods, the advantages of these nanomaterials are high surface area, simplicity of synthesis, high stability, and catalytic activity. The current study presents an overview of organic-inorganic hybrid nanoflowers and their applications in enzymatic catalysis.

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Hemin-incorporated nanoflowers as enzyme mimics for colorimetric detection of foodborne pathogenic bacteria

Cited by 43 publications

References 34 publications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles

Nanoflowers: A New Approach of Enzyme Immobilization

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