Hemmung der anaphylaktischen histaminasefreisetzung durch protamin beim meerschweinchen

Histaminase Liberating and Anticoagulant Activity of Heparin Released in Anaphylactic Shock in the Guinea Pig

“…(3) The blood heparin level, in anaphylactic shock, cor responds to blood levels obtained by injection of heparin doses which arc sufficient to release histaminase (H ahn, G iertz, and K rull 1967). (4) Small doses of protamine sulphate (though this sub stance is by itself a histaminase liberator when applied in high doses) inhibit the histaminase liberation caused either by heparin injections or by anaphylactic shock (H ahn and Schmutzler, 1967;H ahn, Schmutzler, Seseke, Giertz and Bernauer, 1966).…”

Studies of Protracted Shock in Guinea Pigs

“…The histaminase activity is exerted by tissue bound histaminase as well as by plasma histaminase the latter being released from the liver into the plasma during the shock. The histaminase release from the liver is mediated by heparin [H ahn and Schmutzler, 1967]. The histaminolytic activity of liver homogenates was shown to increase during the acute shock phase and then to decrease reaching a minimum after about 40 min [M itze, Prohle, Degand and H ahn, 1970], The increased plasma histamin ase activity was found at a nearly constant level when estimated during a time interval of 5-80 min after antigen injection.…”

Immune Reactions to <i>Nippostrongylus brasiliensis</i> in the Rat

“…Blood was mixed with sodium oxalate and 1 ml of the plasma was diluted with 17 ml Sorensen phosphate buffer 0.067 m, pH 7.0. To the prewarmed solution 20 fig of histamine base (as dihydrochloride) dissolved in 2 ml of the same buffer solution were added and the incubation allowed to proceed at 37° C [5]. For histamine assay, samples were withdrawn immediately after addition of histamine, and 15, 30 and 60 min thereafter.…”

“…For histamine assay, samples were withdrawn immediately after addition of histamine, and 15, 30 and 60 min thereafter. Further details and calculation of histaminase activity were performed as de scribed by Schmutzler [5].…”