Hemocompatibility of titania nanotube arrays

Smith, Barbara S.; Yoriya, Sorachon; Grissom, Laura; Grimes, Craig A.; Popat, Ketul C.

doi:10.1002/jbm.a.33004

Cited by 4 publications

(11 citation statements)

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“…It was our aim to examine which diameter of NTs is more appropriate for protein binding and more suitable (long-term) as nanostructured implant material. Implanted material in contact with blood becomes adhered to plasma proteins 13,76 once embedded in the body. 13 Plasma is rich in proteins, carbohydrates, and lipids, as well as lipoprotein and other combinations of complexes; however, when studying clinically relevant proteins, our aim was to first determine their interactions with NTs' surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have shown binding of different protein or peptide interlayers, such as growth factors, 18 albumin, fibrinogen, IgG, 13,19 fibronectin, 20 collagen type 1, 21 BMP-2, 18,22 RGD peptide, 23 and laminin-derived peptide, 24 to TiO 2 nanotubes (NTs), which could provide for crucial information concerning their integration into the body. The majority of the more recent investigations studied the combination of different cell types with different prebound proteins 25,26 on TiO 2 NTs and found that binding is cell-specific and cannot be generalized.…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the more recent investigations studied the combination of different cell types with different prebound proteins 25,26 on TiO 2 NTs and found that binding is cell-specific and cannot be generalized. However, reports on hemocompatibility with TiO 2 nanostructured material are rare, with exceptions, such as plasma protein adsorption binding studies by Smith et al 13,27 and binding of collagen type 1 by Yang et al 21 with an explanation of interaction energies and main driving forces.…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…13 The protein binding may be changed/optimized by changing the size parameters of TiO 2 NT arrays. 13 Another research group obtained similar results with binding of collagen type 1 on surfaces of NTs, where faster and higher binding was observed on NTs with larger diameters (100 nm). The main driving force for collagen type 1 binding was physical adsorption, including van der Waals force and hydrogen bonds between the proline residues of collagen type 1 and NTs.…”

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confidence: 99%

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Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Kulkarni

Flašker

Lokar

et al. 2015

IJN

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Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO 2 ) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices.

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Section: Discussionmentioning

confidence: 99%