Hemocyte-derived reactive oxygen intermediate production in four bivalve mollusks

Anderson, Robert S.

doi:10.1016/0145-305x(94)90237-2

Cited by 98 publications

(71 citation statements)

References 10 publications

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“…In many bivalve species, phagocytic cells can be activated by foreign particles, or organisms and their products, to release reactive oxygen species (ROS) including hydrogen peroxide, hydroxyl radical and singlet oxygen [2]. The production of ROS is essential for the elimination of invading viruses, bacteria, fungi and protozoa through the activation of the respiratory burst [3,4]. In addition, ROS particularly hydrogen peroxide, may serve as second messengers in signal transduction pathway by activating the NF-kB transcription factor [5,6].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Wang

Yuan

et al. 2013

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Wang

Yuan

et al. 2013

Fish & Shellfish Immunology

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“…Mercenaria mercenaria (Anderson 1994) and Ruditapes decussatus (Lopez et al 1994). R. decussatus is one of the most important species of this family in Europe, from a commercial point of view.…”

Section: Introductionmentioning

confidence: 99%

Differential phagocytic ability of the circulating haemocyte types of the carpet shell clam Ruditapes decussatus (Mollusca:Bivalvia)

López¹,

Carballal²,

Azevedo³

et al. 1997

Dis. Aquat. Org.

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Phagocytosis of foreign materials by haemocytes is an important aspect of the internal defence of bivalve molluscs. Two main haemocyte types can be distinguished in the haemolymph of the clam Ruditapes decussatus: granulocytes and hyalinocytes. The ability of clam haemocytes to phagocytose zymosan particles, Vibrio P1 cells and trophozoites of the protistan parasite Perkinsus atlant~cus was demonstrated by means of in vitro assays. However, clam haemocytes did not phagocytose atlanticus zoospores in the assays. Granulocytes showed the highest phagocytic capacity in each assay. Phagocytic capacity of haemocytes was not significantly affected by clam age. An ultrastructural study of phagocytosis showed the following sequence of events: engulfment of particles by pseudopods, formation of a phagocytic vacuole, fusion of lysosomes with the phagocytic vacuole, and digestion of the particles giving rise to residual bodies that might be discharged.

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“…So far, in oysters, recognition is mediated by germ-line encoded receptors, such as a β-integrin, involved in hemocyte phagocytosis (18); it was proposed to serve as a receptor for the major plasma protein, the extracellular superoxide dismutase Cg-EcSOD, which contains a RGD cellbinding domain (19). In addition, a broad diversity of immune effectors has been characterized in oyster hemocytes ranging from reactive oxygen species (ROS) (20,21) to AMPs (22)(23)(24).…”

mentioning

confidence: 99%

Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus

Duperthuy

Schmitt

Garzón

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

155

168

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OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for β-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase CgEcSOD, is used as an opsonin mediating the OmpU-promoted phagocytosis through its RGD sequence. Finally, the endocytosed bacteria were found to survive intracellularly, evading the host defense by preventing acidic vacuole formation and limiting reactive oxygen species production. We conclude that (i) V. splendidus is a facultative intracellular pathogen that manipulates host defense mechanisms to enter and survive in host immune cells, and (ii) that OmpU is a major determinant of host cell invasion in Vibrio species, used by V. splendidus LGP32 to attach and invade oyster hemocytes through opsonisation by the oyster plasma Cg-EcSOD.T he oyster pathogen, Vibrio splendidus strain LGP32 was isolated from massive mortality events in the production of Crassostrea gigas oysters (1). However, up to now, little has been known about the route of infection and pathogenic processes of LGP32 (2, 3). A metalloprotease has been associated with toxicity (4, 5) and the outer membrane protein (OMP) OmpU was shown to be a major determinant of LGP32 virulence (6).As bacterial surface components, OMPs are both used by hosts for pathogen recognition and by pathogens for interaction with and invasion of host cells, serving as adhesion proteins (adhesins)

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Hemocyte-derived reactive oxygen intermediate production in four bivalve mollusks

Cited by 98 publications

References 10 publications

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Differential phagocytic ability of the circulating haemocyte types of the carpet shell clam Ruditapes decussatus (Mollusca:Bivalvia)

Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus

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