Hemocyte values in healthy blue crabs, Callinectes sapidus, and crabs infected with the amoeba, Paramoeba perniciosa

Sawyer, Thomas K.; Cox, Robert L.; Higginbottom, Mark

doi:10.1016/0022-2011(70)90188-6

Cited by 19 publications

(7 citation statements)

References 13 publications

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“…The characteristics of the three haemocyte morphotypes of the mud crab described here were comparable to most of the decapod haemocyte types investigated so far (Sawyer et al, 1970;Bodammer, 1978;Mix & Sparks, 1980;Martin & Graves, 1985). As observed previously (Bauchau, 1981;Hose et al, 1990;Gargioni & Barracco, 1998), we have also identified hyaline haemocytes having granules in S. serrata.…”

Section: Morphological Characterisationsupporting

confidence: 89%

Microscopic and cytochemical characterisation of haemocytes of the mud crab Scylla serrata (Forskål, 1775) (Decapoda, Portunidae)

Kumar

Deepika

Arumugam

et al. 2013

Crustac

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Haemocytes of the mud crab Scylla serrata (Forskål, 1775) were characterised based on morphological features using light and electron microscopy, and cytochemistry. The cells were identified as hyaline, semigranular and granular haemocytes. Hyaline cells were the smallest haemocytes among the three types identified, having the highest nucleo-cytoplasmic ratio. The cells showed a number of cytoplasmic organelles and also contained a few small as well as large-sized granules. Semigranular haemocytes possessed moderate numbers of large-sized granules or numerous small-sized granules and comparatively less numbers of organelles. Granular haemocytes were the largest haemocytes with the lowest nucleo-cytoplasmic ratio and contained many large-sized granules. Cytoplasmic organelles were least observed in the granular haemocytes. These three haemocyte morphotypes constituted 60, 21 and 19%, respectively, of the total haemocyte population, while the total haemocyte count was 7.31 × 106 to 7.18 × 107 with a mean of 2.86 × 107 cells ml−1. In cytochemical studies performed to localize carbohydrates, lipids and prophenol oxidase, all the haemocyte types were positive for PAS and toluidine blue, indicating the presence of mucopolysaccharides, whereas semigranular and granular haemocytes were rich in carbohydrates and lipid moieties. Besides, prophenol oxidase was localised within the granules of semigranular and granular haemocytes. Hyaline haemocytes showed an abundance of well differentiated cytoplasmic organelles and granules, and there was a distinct differentiation between semigranular and granular haemocytes in terms of granules and organelles. This is the first report of the characterisation of haemocytes of the mud crab.

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Section: Morphological Characterisationsupporting

confidence: 89%

Microscopic and cytochemical characterisation of haemocytes of the mud crab Scylla serrata (Forskål, 1775) (Decapoda, Portunidae)

Kumar

Deepika

Arumugam

et al. 2013

Crustac

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“…Two separate aliquots of the hemocyte suspension were counted and averaged for each crab. Normal blue crab hemocyte counts are highly variable (Sawyer et al, 1970;Holman et al, 2004;Macey et al, 2008), ranging from 4 × 10 6 to 128 × 10 6 THC mL −1 with 85% of values falling between 10 × 10 6 and 70 × 10 6 THC mL −1 (Sawyer et al, 1970). Crabs used in the present study averaged approximately 13 × 10 6 to 17 × 10 6 THC mL −1 hemolymph.…”

Section: Health Assessment Of Crabs Prior To Experimentationmentioning

confidence: 72%

Effects of salinity on the accumulation of hemocyte aggregates and bacteria in the gills of Callinectes sapidus, the Atlantic blue crab, injected with Vibrio campbellii

Ikerd¹,

Burnett²,

Burnett³

2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…According to Sawyer et al. (), 85% of wild caught blue crabs have a total hemocyte count between 10 × 10 6 and 70 × 10 6 hemocytes mL −1 , so crabs outside of this range were excluded from experiments.…”

Section: Methodsmentioning

confidence: 99%

Effects of Hypoxia and Hypercapnic Hypoxia on Oxygen Transport and Acid–Base Status in the Atlantic Blue Crab, Callinectes sapidus , During Exercise

Lehtonen

Burnett

2016

J. Exp. Zool.

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The responses of estuarine invertebrates to hypoxic conditions are well established. However, many studies have investigated hypoxia as an isolated condition despite its frequent co-occurrence with hypercapnia (elevated CO ). Although many studies suggest deleterious effects, hypercapnia has been observed to improve blue crab walking performance in hypoxia. To investigate the physiological effects of combined hypercapnic hypoxia, we measured Po , pH, [l-lactate], Pco , and total O in pre- and postbranchial hemolymph sampled from blue crabs during walking exercise. Crabs walked at 8 m min on an aquatic treadmill in normoxic (100% air saturation), moderately hypoxic (50%), and severely hypoxic (20%) seawater with and without the addition of hypercapnia (about 2% CO ). Respiration was almost completely aerobic in normoxic conditions, with little buildup of lactate. During exercise under severe hypoxia, lactate increased from 1.4 to 11.0 mM, indicating a heavy reliance on anaerobic respiration. The O saturation of arterial hemocyanin was 47% in severe hypoxia after 120 min, significantly lower than in normoxia (80%). However, the addition of hypercapnia significantly increased the percentage saturation of arterial hemocyanin in severe hypoxia to 92% after 120 min of exercise, equivalent to normoxic levels. Hypercapnia in severe hypoxia also caused a marked increase in hemolymph Pco (around 1.1 kPa), but caused only a minor decrease in pH of 0.1 units. We suggest that the improved O saturation at the gills results from a specific effect of molecular CO on hemocyanin oxygen binding affinity, which works independently of and counter to the effects of decreased pH.

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Hemocyte values in healthy blue crabs, Callinectes sapidus, and crabs infected with the amoeba, Paramoeba perniciosa

Cited by 19 publications

References 13 publications

Microscopic and cytochemical characterisation of haemocytes of the mud crab Scylla serrata (Forskål, 1775) (Decapoda, Portunidae)

Microscopic and cytochemical characterisation of haemocytes of the mud crab Scylla serrata (Forskål, 1775) (Decapoda, Portunidae)

Effects of salinity on the accumulation of hemocyte aggregates and bacteria in the gills of Callinectes sapidus, the Atlantic blue crab, injected with Vibrio campbellii

Effects of Hypoxia and Hypercapnic Hypoxia on Oxygen Transport and Acid–Base Status in the Atlantic Blue Crab, Callinectes sapidus , During Exercise

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