Hemodynamic patterns associated with activation of bradykinin-sensitive pericardial afferents

Abstract

The heart is endowed with reflexogenic areas capable of powerful blood pressure responses. Relatively little work has studied the hemodynamic mechanisms underlying these responses and whether these are sexually dimorphic. We hypothesized that activation of bradykinin-sensitive pericardial afferents would produce a sexually dimorphic cardiac output response. Male and female Sprague Dawley rats were anesthetized and instrumented with catheters for recording arterial pressure, with an aortic arch flow probe to record cardiac output and with a catheter in the pericardial sac. Mean arterial pressure ( MAP ), cardiac index ( CI ) and total peripheral resistance index ( TPRI ) responses to pericardial bradykinin injection (0.1, 1 μg/kg) were recorded. Pericardial bradykinin injection caused similar increases in MAP in male and female rats. However, the underlying hemodynamic patterns varied considerably. We identified a cluster of CI responders and TPRI responders in both male and female rats. Within CI responders, females exhibited greater CI increases than males. Conversely, in TPRI responders, males exhibited a greater TPRI increase than females. We conclude that aggregate activation of bradykinin-sensitive pericardial afferents is associated with a relatively uniform pressor response but different hemodynamic patterns with males exhibiting a more robust vascular response and females a more robust cardiac output response.

Venoconstrictor responses to activation of bradykinin‐sensitive pericardial afferents involve the region of the hypothalamic paraventricular nucleus

“…Nevertheless, it seems logical that a CSAR‐driven increase in MCFP will lead to an increase in cardiac output. Indeed, CSAR stimulation was reported to increase cardiac output by approximately 14% in the rat (Wang et al, 2017) and we reported that pericardial BK injection increased cardiac output by 12%–20% in male and female rats, respectively (Martin et al, 2022). Thus, activation of bradykinin‐sensitive afferents in the pericardial space induces a venoconstrictor response of sufficient magnitude to influence systemic hemodynamics.…”