Hemoglobin-Based Oxygen Carriers Incorporating Nanozymes for the Depletion of Reactive Oxygen Species

Abstract

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Synthesis of Nanoparticles Fully Made of Hemoglobin with Antioxidant Properties: A Step toward the Creation of Successful Oxygen Carriers

“…No further decrease was detected for the longest incubation time. This result is not surprising and confirms Hb’s scavenging properties due to its oxidation to metHb . Upon coating with PDA, the resulting Hb/PDA-NPs promoted a ∼70% decrease after only 10 min of incubation.…”

“…This result is not surprising and confirms Hb's scavenging properties due to its oxidation to metHb. 53 Upon coating with PDA, the resulting Hb/PDA-NPs promoted a ∼70% decrease after only 10 min of incubation. No further decrease in nAbs could be detected for the longer incubation periods, indicating saturation of the system.…”

“…Although, in this study, such an increase in nCV was nonsignificant, it is in agreement with other reports that have shown enhanced nCV readings, which have been attributed to an additional supply of oxygen by the HBOCs, which could facilitate the viability of the cells. 15,53 Although nonsignificant when compared to the cells-only group, a decrease of ∼15% in nCV was observed for the highest concentration, suggesting that 30 000 NPs μL −1 would be the highest tolerable concentration.…”

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

“…Their ROS-scavenging activity was also proposed to treat rheumatoid arthritis [ 132 ], and to adjuvate in the treatment of traumas whereby blood transfusions are not an option, and nanocarriers to deliver hemoglobin are being studied as a possible alternative [ 133 ]. Ceria NPs have been stabilized with cyclodextrin that coated the NPs and encapsulated dithranol for the combined therapy of psoriasis, and they showed effective mitigation of ROS-induced damage in vitro and in vivo in a mouse model [ 34 ].…”

Optimization of Hemoglobin Encapsulation within PLGA Nanoparticles and Their Investigation as Potential Oxygen Carriers

“…Numerous encapsulation platforms are currently being investigated and, to date, Hb has been successfully entrapped within liposomes, polymersomes, hydrogels, and metal-organic framework-based particles [7][8][9]. Amongst them, our group has reported the encapsulation of Hb within a novel carrier which displays both antioxidant and stealth properties [10]. Specifically, Hb and cerium oxide nanoparticles (NPs), with the ability to scavenge reactive oxygen species, were deposited onto a poly(lactide-co-glycolide) (PLGA) core, using the layer-by-layer technique.…”