Hemoglobin Concentration and Acetylcholinesterase Activity of Oligochaetes in Relation to Lead Concentration in Spiked Sediments from Ignacio Ramirez Reservoir

Martínez‐Tabche, Laura; Ortega, Maria De Los Angeles Grajeday; Mora, Beatriz Ramirez; Faz, Celia German; López, Eugenia López; Galár-Martínez, Marcela

doi:10.1006/eesa.2001.2030

Cited by 19 publications

(8 citation statements)

References 15 publications

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“…These organisms are thought to have an excellent potential for evaluation of metal toxicity because of their short life cycle and high sensitivity to these metals (Bouché et al, 2000;Martínez-Tabche et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

Ortega¹,

Ordoñez²,

Favari

et al. 2011

Int. J. Morphol.

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SUMMARY:The effects of sublethal concentrations of cadmium (0.64 µg/L), iron (0.043 mg/L) and zinc (0.31 mg/L) and a mixture of these metals on succinate dehydrogenase (SD) and alkaline phosphatase (AP) activity and on structural changes in the mitochondria of epithelium cells of the digestive tract were examined in the oligochaete Limnodrillus hoffmeisteri after 96 h of exposure in artificial sediments. SD activity was significantly inhibited, particularly in treatments with Cd alone (92.57%), while AP increased its activity with Cd alone (73.23%). However, when this metal was mixed with Fe and Zn, the inhibition of SD activity was lower (67.82%) than with Cd alone, showing an antagonistic effect and AP increased its activity (73.26%). Mitochondria were structurally damaged by exposure to Cd alone. However, in the metal mixtures, the toxic effects may exert interactive effects eliciting a less structural damage in the mitochondria of epithelium cells of the digestive tract than when Cd is alone.

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Section: Introductionmentioning

confidence: 99%

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

Ortega¹,

Ordoñez²,

Favari

et al. 2011

Int. J. Morphol.

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“…The measurement of AChE inhibition in marine organisms has been widely used as an indicator of neurotoxicity caused by environmental contamination. AChE activity has been demonstrated to be strongly inhibited by organophosphate and carbamate pesticides, and also by metals and surfactant agents (Guilhermino et al, 2000;Gill et al 1991;Martinez-Tabche et al 2001;Monserrat et al 2002;Bainy et al2006), causing dysfunction of the central nervous system. In mussels M. galloprovincialis, AChE activity was signi cantly reduced after exposure to Bap (Akcha et al, 2000;Banni et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of Acute Benzo[a]pyrene Exposure on Blood Clam Tegillarca Granosa: Histological Changes, Oxidative Stress, Neurotoxicity and DNA Hypomethylation

Guo

Feng

et al. 2021

Preprint

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The blood clam is being developed into a model bivalve molluscs for assessing and monitoring marine pollution on the offshore seabed. However, the information on the response of blood clam to PAHs, an organic pollutant usually deposited in submarine sediment, remains limited. Herein, we employed multiple biomarkers, including histological changes, oxidative stress, neurotoxicity and global DNA methylation, to investigate the effects of Bap exposure under laboratory conditions on blood clams and its potential mechanisms. Acute Bap exposure can induce significant morphological abnormalities in gills as shown through hematoxylin-eosin (H.E) staining, providing an intuitive understanding on the effects of Bap on the structural organization of blood clams. Meanwhile, the oxidative stress was significantly elevated as manifested by the increase of antioxidants activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione-s-transferase (GST), lipid peroxidation (LPO) level and 8-hydroxy-2’-deoxyguanosine (8-OHdG) content. The neurotoxicity was also strengthened by Bap toxicity manifested as inhibited acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) activities. In addition, the global DNA methylation level was investigated, and a significant DNA hypomethylation was observed in Bap exposed blood clams. The correlation analysis showed that the global DNA methylation was negatively correlated with antioxidants (SOD, CAT and POD) activities, but positively correlated choline enzymes (AChE and ChAT) activities. These results collectively suggested that acute Bap exposure can cause damage in gills structures in blood clams possibly by generating oxidative stress and neurotoxicity, and the global DNA methylation was inhibited to increase the transcriptional expression level of antioxidants genes and consequently elevate antioxidants activities against Bap toxicity. These results are hoped to shed some new light on the study of ecotoxicology effect of PAHs on marine bivalves.

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“…The oligochaete L. hoffmeisteri is a benthic organism living in aquatic systems rich in organic matter. It is relatively resistant to heavy metals and plays an important role as a source of nourishment for other organisms in the food chain [12,20,21].…”

Section: Methodsmentioning

confidence: 99%

Cadmium, iron, and zinc uptake individually and as a mixture by Limnodrillus hoffmeisteri and impact on adenosine triphosphate content

Ortega

López‐López

Perotzi

et al. 2008

Enviro Toxic and Chemistry

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Heavy metals, such as Fe, Zn, and Cd, are major pollutants in aquatic ecosystems. These toxicants usually are present in these ecosystems in the form of mixtures, and their effect on exposed organisms is the result of interactions occurring between the metals as well as with other pollutants and environmental factors. The present study evaluated the uptake of Cd, Fe, and Zn individually and as a mixture and their effect on adenosine triphosphate (ATP) content in Limnodrillus hoffmeisteri, an important aquatic organism given its resistance to large amounts of these xenobiotics. Test organisms were exposed for 96 h to sediment treated with each of these metals individually and as a mixture. The metal concentrations used were equivalent to the zero lethal concentration of these pollutants as determined in a previous study. At the end of the exposure period, metal concentration in medium and organism as well as ATP content in the latter were quantified. Metal uptake was measured by determining the bioconcentration factor at 96 h. All three metals elicited an important decrease in ATP content when acting singly and as part of a mixture. When present as a mixture, their uptake and effect on L. hoffmeisteri are modified. Therefore, a joint-action toxicity approach should be used for toxicity evaluations involving these pollutants.

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Hemoglobin Concentration and Acetylcholinesterase Activity of Oligochaetes in Relation to Lead Concentration in Spiked Sediments from Ignacio Ramirez Reservoir

Cited by 19 publications

References 15 publications

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

Effects of Acute Benzo[a]pyrene Exposure on Blood Clam Tegillarca Granosa: Histological Changes, Oxidative Stress, Neurotoxicity and DNA Hypomethylation

Cadmium, iron, and zinc uptake individually and as a mixture by Limnodrillus hoffmeisteri and impact on adenosine triphosphate content

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