Hemoglobin function in stored blood

Bunn, H. Franklin; May, Mary H.; Kocholaty, Walter F.; Shields, Charles E.

doi:10.1172/jci105987

Cited by 205 publications

(67 citation statements)

References 24 publications

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“…The studies reported here together with the studies relating changes in 2,3-DPG levels to the P55 of blood in such diverse situations as anemia with and without intrinsic defects in red cell glycolysis (28,29), altitude change (5,30), and blood storage (31,32), as well as the demonstrated ability of added 2,3-DPG to correct the differences between the oxygen affinity of whole blood and hemolysate (3) establish the important role of the organic phosphates in the regulation of oxygen release to the tissues. Throughout the physiologic range (2000-10,000 msmoles of DPG/ml of RBC's), change in oxygen affinity as measured by the P50 was found to be linear with respect to the 2,3-DPG concentration; 430 m/moles of 2,3-DPG changing the P60 by 1 mm Hg.…”

Section: Discussionmentioning

confidence: 77%

The effects of deoxygenation of adult and fetal hemoglobin on the synthesis of red cell 2,3-diphosphoglycerate and its in vivo consequences

Oski¹,

Gottlieb²,

Miller³

et al. 1970

J. Clin. Invest.

106

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A B S T R A C T Patients over 1 month of age with arterial oxygen pressures of less than 60 mm Hg were found to have elevated red cell 2,3-diphosphoglycerate (2,3-DPG) levels and blood with a decreased affinity for oxygen. The increase in 2,3-DPG was proportional to the degree of hypoxemia. In patients under 1 month of age this relationship was not observed. Red cells from adults, but not newborns, showed rapid increases in 2,3-DPG when incubated under nitrogen. Adult, but not fetal, deoxyhemoglobin was shown to facilitate in vitro synthesis of 2,3-DPG by binding this organic phosphate and relieving the product inhibition of 2,3-DPG mutase.Throughout a wide range change in oxygen affinity as measured by the Pao is linear with respect to the 2,3-DPG concentration; a change of 430 mnumoles of 2,3-DPG/ml of red blood corpuscles (RBC) resulting in a change of the Pao of 1 mm Hg. It appears that the 2,3-DPG of the adult's red cells responds rapidly to metabolic and environmental influences and in turn effects metabolism and the cellular environment. Many of these effects are not shared by the red cells of the newborn.

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Section: Discussionmentioning

confidence: 77%

The effects of deoxygenation of adult and fetal hemoglobin on the synthesis of red cell 2,3-diphosphoglycerate and its in vivo consequences

Oski¹,

Gottlieb²,

Miller³

et al. 1970

J. Clin. Invest.

106

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show abstract

“…2B). DPG concentrations are said to be increased in reticulocyte-rich blood (10,42) (13,14), is also difficult to explain on DPG concentration alone, for oxygen affinities as low as those found in these patients (A A, [12][13][14] were not found at the DPG levels found in their red cells (6.6-14.9 /smole/ml).…”

Section: Discussionmentioning

confidence: 99%

“…Akerblom, de Verdier, Garby, and Hogman (9) and' Bunn, May, Kochalaty, and Shields (10) found that the high oxygen affinity of stored blood could be related to its low content of DPG and ATP, and that restoration of DPG and ATP levels after incubation of stored red cells with inosine was associated with a return of oxygen affinity to normal levels. Decreased oxygen affinity of the blood of persons residing at high altitude was related to an increased concentration of DPG within their red cells (11,12).…”

Section: Introductionmentioning

confidence: 98%

Effect of 2,3-diphosphoglycerate on oxygen affinity of blood in sickle cell anemia

Charache¹,

Grisolı́a²,

Fiedler³

et al. 1970

J. Clin. Invest.

115

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“…In some of these states, such as anemia, hypoxemia, and cardiac failure, there is a decrease in blood oxygen affinity (1)(2)(3). Under other circumstances, such as occur after transfusion of stored blood, oxygen affinity is increased (4)(5)(6)(7). However, the importance of such changes on oxygen availability to cells is unknown.…”

Section: Introductionmentioning

confidence: 99%

Effect of Increased Blood Oxygen Affinity on Work Performance of Rats

Woodson¹,

Wranne²,

Detter³

1973

J. Clin. Invest.

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A B S T R A C T Influence of altered blood oxygen affinity on maximal performance ability was evaluated in trained rats exercising to exhaustion in a graded treadmill test. Modification of blood oxygen affinity was achieved both by 2,3-diphosphoglycerate depletion, accomplished by exposure of animals to C02 and by exchange transfusion with blood exposed to bisulfite or stored in acid citrate dextrose, and by carbamylation of hemoglobin, produced by exchange transfusion of blood incubated with potassium cyanate. A decrease in oxygen tension at half-saturation of hemoglobin (P5o) from 36 to 23 mm Hg produced a decrease in resting central venous oxygen pressure of about 12 mm Hg. During exercise it caused an average decrease in work performance of about 10%, which was equivalent to that performance decrement caused by a decrease in hemoglobin concentration of approximately 10%. When superimposed on anemia, this change in blood oxygen affinity again caused a similar decrease in performance over and above that due to anemia alone. A marked rightward shift of the in vivo oxygen dissociation curve during severe exercise may have compensated for the reduced in vitro P5o.

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Hemoglobin function in stored blood

Cited by 205 publications

References 24 publications

The effects of deoxygenation of adult and fetal hemoglobin on the synthesis of red cell 2,3-diphosphoglycerate and its in vivo consequences

The effects of deoxygenation of adult and fetal hemoglobin on the synthesis of red cell 2,3-diphosphoglycerate and its in vivo consequences

Effect of 2,3-diphosphoglycerate on oxygen affinity of blood in sickle cell anemia

Effect of Increased Blood Oxygen Affinity on Work Performance of Rats

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