Hemoglobin Regeneration Efficiency in Anemic Rats: Effects on Bone Mineral Composition and Biomechanical Properties

Abstract

This study reports the effects of dietary iron (Fe) deficiency and recovery on bone mineral composition and strength in anemic rats submitted to a hemoglobin (Hb) repletion assay. Weanling male Wistar rats were fed a low-Fe diet (12 mg/kg) for 15 days followed by 2 weeks of Fe repletion with diets providing 35 mg Fe/kg as either ferrous sulfate (n = 8) or ferric pyrophosphate (FP; n = 12). At final day of each period (depletion and repletion), Fe-adequate animals were also euthanized. Iron status (blood Hb, Hb Fe pool, Hb regeneration efficiency), tibia mineral concentrations (Ca, Mg, Fe, Cu, and Zn) and biomechanical properties were evaluated. Iron-deficient rats had lower tibia Fe and Mg levels and bone strength when compared to controls. Yield load and resilience were positively related to tibia Mg levels (r = 0.47, P = 0.02 and r = 0.56, P = 0.004, respectively). Iron repletion did not recover tibia Mg concentrations impaired by Fe deficiency. Moreover, bone elastic properties were negatively affected by FP consumption. In conclusion, bone mineral composition and strength were affected by Fe deficiency, whereas dietary Fe source influenced tibia Mg and resistance in the period during which rats were recovering from anemia.

Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing

“…The animals were kept under natural conditions of light, humidity, and temperature at the Animal House of Center of Biophotonics of the Federal University of Bahia during the experimental period. Iron deficiency anemia was induced by feeding the animals with a special pelleted iron-free diet (no iron-AIN 93-G, Rhoster, SP, Brazil) during 15 days [12]. Anemia was confirmed by blood testing.…”

Correlation of hemoglobin with osteoporosis in elderly Chinese population: A cross-sectional study

“…Hemoglobins (Hbs) are large and complex protein molecules, and the Hb level is related to nutrients from dietary intakes (12). Animal experiments have shown that lower Hb level is related to the impaired bone turnover and poor bone strength (13)(14)(15). Dietary iron deficiency in Wistar rats can induce the decrease of Hb concentration, accompanied by decreased serum osteocalcin concentration, bone mineral content, BMD, and mechanical strength of the femur (14).…”

Hemoglobin and hip fracture risk in older non-Hispanic white adults

“…Low hemoglobin has been linked to impaired bone turnover and poor bone strength in animal models [1–3], and a small number of studies in humans have reported an association between low hemoglobin and low BMD [4–6]. Although anemia is common in the elderly [7], to date only two prospective studies and one retrospective open-cohort study have examined the relationship between low hemoglobin and risk of fracture or injurious falls that included fracture [8–10].…”