Hemólisis en las muestras para diagnóstico

Rioja, Rubén Gómez; Kirchner, María Jesús Alsina; Funes, Virtudes Álvarez; Meseguer, Nuria Barba; Rius, Mariano Cortés; Diaz, María Antonia Llopis; Brú, Cecilia Martínez

doi:10.1016/j.labcli.2009.08.002

Cited by 16 publications

(16 citation statements)

References 56 publications

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“…Este criterio, utilizado por muchos fabricantes en sus estudios de evaluación, es más permisivo en las magnitudes con pequeña variabilidad biológica y puede ser menos permisivo en aquellas con elevada variabilidad intraindividual o interindividual (1,6) .…”

Section: Discussionunclassified

“…Los índices pueden ser usados para detectar la interferencia generando tablas de tolerancia, con índices de decisión que indican cuándo hay una interferencia. En nuestro medio, a pesar de contar con estos equipos, no se hace uso de esta tecnología por desconocimiento o por la excusa de consumo de tiempo de proceso del analizador y necesidad de reactivos específicos en algún caso (6,8,13) .…”

Section: Discussionunclassified

“…La Organización Mundial de la Salud, con sustentación en las recomendaciones de la Sociedad Alemana de Química Clínica, define interferencia clínicamente relevante cuando se supera el error sistemático deseable (6) .…”

Section: Introductionunclassified

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Interferencia causada por hemólisis en la determinación de 25 constituyentes bioquímicos en el autoanalizador ADVIA 1800

Saldaña

Moisés²

2016

An Fac med

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ResumenIntroducción. La interferencia por hemólisis es la principal causa de rechazo preanalítico de muestras de suero en el laboratorio clínico. Objetivos. Conocer y cuantificar la posible interferencia producida por hemólisis en la medición rutinaria de 25 constituyentes bioquímicos en el autoanalizador ADVIA 1800, empleando para ello el criterio de interferencia clínicamente relevante, cuando se supera el máximo error sistemático deseable. Diseño. Estudio descriptivo comparativo. Institución. Hospital Edgardo Rebagliati Martins, EsSalud, Lima, Perú. Material biológico. Muestras sanguíneas proveniente de sujetos voluntarios. Intervenciones. Se añadieron cantidades crecientes de hemoglobina (0,26 g/L, 0,53 g/L, 1,05 g/L, 2,10 g/L, 3,25 g/L, 4,30 g/L y 5,25 g/L) a siete diferentes alícuotas de una mezcla de sueros y se determinó en ellas por duplicado la influencia del interferente en los 25 constituyentes. Se siguió el protocolo de la Sociedad Española de Química Clínica. Principal medida de resultados. Porcentaje relativo de desviación de la concentración del constituyente por influencia de la hemólisis, con respecto a la muestra sin interferente. Resultados. Los constituyentes urea, creatinina, ácido úrico, bilirrubina total, colesterol HDL, colesterol LDL, triglicéridos, calcio y gammaglutamiltransferasa no presentaron interferencia, mientras que se observó interferencia para glucosa, proteínas, albúmina, colesterol, potasio, fósforo, magnesio, deshidrogenasa láctica, creatinfosfoquinasa, aspartato aminotransferasa, alanino aminotransferasa, lipasa, sodio, cloro, fosfatasa alcalina y amilasa. were added to seven different aliquots of sera mixture and influence of interfering influence in 25 constituents was determined by duplicate. The Spanish Society of Clinical Chemistry protocol was followed. Main outcome measure. Hemolysisrelated relative percentage deviation of the constituent concentration compared with the sample without interference. Results. Urea, creatinine, uric acid, total bilirubin, HDL cholesterol, LDL cholesterol, triglycerides, calcium, and gamma glutamyl transferase showed no interference. Interference was observed for glucose, protein, albumin, cholesterol, potassium, phosphorus, magnesium, lactic dehydrogenase, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase, lipase, sodium, chlorine, alkaline phosphatase and amylase. Conclusions. Out of 25 constituentss studied, 16 had clinical significant interference. It is recommended that each laboratory investigate this interference effects using their own methods, reagents or instruments.

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Section: Discussionunclassified

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Interferencia causada por hemólisis en la determinación de 25 constituyentes bioquímicos en el autoanalizador ADVIA 1800

Saldaña

Moisés²

2016

An Fac med

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“…When red cells are broken, hemoglobin is released and the serum acquires a reddish color proportionally which depends on the erythrocytes degraded, which indicates the degree of hemolysis (Gómez Rioja et al 2009). The effect of the hemolysis in wild boar sera has been poorly documented (Boadella and Gortazar 2011), and no spectrophotometric data on hemolysis have been reported in game species.…”

Section: Discussionmentioning

confidence: 99%

Blood sampling by puncture in the cavernous sinus from hunted wild boar

et al. 2013

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A new method of sampling based on the extraction of blood from the cavernous sinus of the dura mater has been assessed in hunted wild boar. Blood from 139 animals was obtained by two different extraction methods: the harvesting from thoracic cavity (TC) and intracavernous venipuncture (IV). Sera obtained by the IV method had higher volume (mean 2.85 vs 1.85 ml), were less hemolytic (mean absorbance at 450 nm: 1.01 vs 2.41 nm). A higher number of samples and a higher proportion of sera collected by IV (90.6 %) compared to those obtained using the TC method (78.4 %), could be analyzed against Aujeszky's disease using blocking ELISA. No statistically significant differences in seroprevalences between samples obtained using both extraction methods were observed. The results obtained indicate that the IV is an easy, fast, reliable, clean, and safe method to collect blood samples from hunted wild boar, proving a real alternative to the traditional collection method.

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“…With these three types of comments, both the sign of interference and concentration are considered, differentiating when the result of the determination can be provided, and when not, since providing it would lead to errors in clinical interpretation. Likewise, the use of clinically significant interference improves the interpretation that hemolysis produces on laboratory tests, since it is calculated individually for each analyte and not applied in general as in analytical interference (10%) [2]. This study allowed us to compare the results obtained with the information provided by the inserts, founding differences in several of the techniques analyzed (Table 2).…”

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Study of Interference Produced by Haemolysis In 73 Analytical Tests

Douhal

2020

BJSTR

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One of the main objectives of a clinical laboratory is getting accurate values. To quantify the hemolysis, the study of hemolytic index is a widespread practice. Due to a change of analyzers, the present hemolysis interference study is aiming to quantify the possible change in 73 tests performed by different techniques in such equipment: spectrophotometry, indirect potentiometric and immunological techniques. The tests were performed in Roche Diagnostics Cobas 8000 equipment following the protocol of the Spanish Society of Laboratory Medicine (SEQCml). The results were compared with those reported in the inserts provided by Roche Diagnostics of the different techniques. Interference was obtained for the following compounds: amylase, aspartate aminotransferase (AST), direct bilirubin (DBil), creatinine kinase (CK), alkaline phosphatase (ALP), iron, lactate dehydrogenase (LDH), lipase, phosphorus, potassium, triglycerides, folate, ultrasensitive troponin T (hs-cTnT), insulin and parathyroid hormone (PTH). Working with new analyzers, it is important to study the interference of the hemolysis on different analytical techniques, as it has an impact on the medical decision making. In clinical practice, it is recommended to determine the hemolytic index, to detect and quantify the interference by hemolysis, assessing the possible rejection of samples and providing reliable reports helping clinicians to interpret the results.

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Hemólisis en las muestras para diagnóstico

Cited by 16 publications

References 56 publications

Interferencia causada por hemólisis en la determinación de 25 constituyentes bioquímicos en el autoanalizador ADVIA 1800

Interferencia causada por hemólisis en la determinación de 25 constituyentes bioquímicos en el autoanalizador ADVIA 1800

Blood sampling by puncture in the cavernous sinus from hunted wild boar

Study of Interference Produced by Haemolysis In 73 Analytical Tests

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