Hemolymph ammonia and urea and nitrogenous excretions of Scylla serrata at different temperature and salinity levels

Chen, JC; Pg, Chia

doi:10.3354/meps139119

Cited by 29 publications

(8 citation statements)

References 19 publications

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“…Therefore, at higher salinities, the excretion of ammonia was probably lower, with the compound accumulating and reaching toxic levels. This result also corroborates the findings of Chen and Chia (1996) who demonstrated an inverse relationship between ammonia excretion and salinity in the mud crab Scylla serrata.…”

Section: Discussionsupporting

confidence: 92%

Acute toxicity of total ammonia to Macrobrachium rosenbergii postlarvae at different salinity levels

Motta,

Santos,

Dutra

et al. 2024

Braz. J. Biol.

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Nitrogen compounds, particularly ammonium, nitrite and nitrate, are a major problem in shrimp production systems. These compounds can accumulate in the aquatic environment and reach harmful or even lethal levels. Thus, monitoring the levels of nitrogenous compounds such as ammonia and studying their effects on the animals are essential. One tool used for this purpose is acute toxicity testing based on the evaluation of LC50 values. Furthermore, tools that can help improve the performance of aquatic organisms in culture are needed. The present study aimed to evaluate the effect of salinity on the toxicity of total ammonia to postlarvae of the freshwater prawn Macrobrachium rosenbergii. For this purpose, acute toxicity testing (LC50-96h) was performed using 540 postlarvae with a mean weight of 0.13 g and a mean total length of 2.47 cm, divided into 54 experimental units of two liters each. A completely randomized design in a 3×6 factorial scheme was used, combining three salinities (0, 5, and 10 g.L-1) and six total ammonia concentrations (0, 8, 16, 32, 64, and 128 mg.L-1), with three replicates per combination. The LC50 values for M. rosenbergii postlarvae at 24, 48, 72, and 96 h and their respective confidence intervals (95%) were estimated using the trimmed Spearman-Karber method. The results showed that salinities of 5 or 10 g.L-1 did not reduce the acute toxicity of total ammonia.

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Section: Discussionsupporting

confidence: 92%

Acute toxicity of total ammonia to Macrobrachium rosenbergii postlarvae at different salinity levels

Motta,

Santos,

Dutra

et al. 2024

Braz. J. Biol.

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“…However, the results in the present study show that the ammonia‐N excretion of the shrimp is significantly lower at low salinities of 5000–15 000 mg L −1 . We assume that L. vannamei juvenile exposed to lower salinity levels may shift their excretion pattern from ammoniotelism to ureotelism, which has been confirmed in Scylla serrata (Chen & Chia 1996), in P. japonicus (Chen & Chen 1996; Lee & Chen 2003), in P. monodon (Chen, Chen & Cheng 1994) and in Orconectes rusticus (Sharma 1968), when exposed to higher salinity levels. But we did not specifically investigate this aspect, and thus further research is needed to carry out the detailed investigation.…”

Section: Discussionmentioning

confidence: 79%

Effect of salinity on survival, growth, oxygen consumption and ammonia-N excretion of juvenile whiteleg shrimp,Litopenaeus vannamei

Zhang

et al. 2009

Aquaculture Research

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In this study, we tested the lower salinity tolerance of juvenile shrimps (Litopenaeus vannamei) at a relatively low temperature (20 1C). In the ¢rst of two laboratory experiments, we ¢rst abruptly transferred shrimps (6.91 AE 0.05 g wet weight, mean AE SE) from the rearing salinity (35000 mg L À 1 ) to salinities of 5000, 15000, 25000, 35000 (control) and 40 000 mg L À 1 at 20 1C. The survival of L. vannamei juvenile was not a¡ected by salinities from 15000 to 40 000 mg L À 1 during the 96-h exposure periods. Shrimps exposed to 5000 mg L À 1 were signi¢cantly a¡ected by salinity, with a survival of 12.5% after 96 h. The 24-, 48-and 96-h lethal salinity for 50% (LS 50 ) were 7020,8510 and 9540 mg L À 1 respectively. In the second experiment, shrimps (5.47 AE 0.09 g wet weight, mean AE SE) were acclimatized to the different salinity levels (5000, 15000, 25000, 35000 and 40 000 mg L À 1 ) and then maintained for 30 days at 20 1C. Results showed that the survival was signi¢cantly lower at 5000 mg L À 1 than at other salinity levels, but the ¢nal wet weight under 5000 mg L À 1 treatment was signi¢cantly higher than those under other treatments (Po0.05). Feed intake (FI) of shrimp under 5000 mg L À 1 was signi¢cantly lower than those of shrimp under 150 004 0 000 mg L À 1 ; food conversion e⁄ciency (FCE), however, showed a contrasting change (Po0.05). Furthermore, salinity signi¢cantly in£uenced the oxygen consumption rates, ammonia-N excretion rates and the O/N ratio of test shrimps (Po0.05). The results obtained in our work provide evidence that L. vannamei juveniles have limited capacity to tolerate salinities o10 000 mg L À 1 at a relatively low temperature (20 1C). Results also show that L. vannamei juvenile can recover from the abrupt salinity change between 15000 and 40 000 mg L À 1 within 24 h. Low salinity and temperature a¡ected survival of whiteleg shrimp P Zhang et al. Low salinity and temperature a¡ected survival of whiteleg shrimp P Zhang et al. Low salinity and temperature a¡ected survival of whiteleg shrimp P Zhang et al. Low salinity and temperature a¡ected survival of whiteleg shrimp P Zhang et al.

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“…One example of a scenario causing a sudden reduction in salinity is heavy rainfall in ponds, which leads to S. paramamosain mortality. In recent years, several studies have been published on the salinity tolerance of S. paramamosain ([6, 21]; Hai et al, 1998; [22–24]. ).…”

Section: Introductionmentioning

confidence: 99%

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Wang

Wei

Tang

et al. 2019

International Journal of Genomics

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Scylla paramamosain is a commercially important species distributed along the coast of southern China and other Indo-Pacific countries. Sudden salinity drop exceeding the adjustment capability of S. paramamosain can result in damage or even mortality. In our previous study, we had analyzed the mechanism of adapting sudden drop in salinity from the level of transcriptomics and proteomics, respectively. This study performed a correlation analysis of RNA sequencing transcriptomics and iTRAQ proteomics in order to investigate the adaptation mechanisms to sudden salinity drop from 23‰ to 3‰. There were 3954 correlations and a total of 15 correlations for differentially expressed proteins (DEPs) and differentially expressed genes (DEGs) from proteomics and transcriptomics. The correlation between DEPs and DEGs was 0, and the Spearman correlation coefficient of the same trend correlation for DEPs and DEGs was the highest, reaching 0.9080. KEGG pathway enrichment correlation revealed that protein digestion and absorption (Ko04974), proximal tubule bicarbonate (Ko04964), and bile secretion (Ko04976) played important roles in Na+/H+ and Na+/K+ exchange. In addition, important genes related to osmoregulation, such as ion transport and carbonic anhydrase, were also detected in the correlation analysis for same trend DEPs_DEGs. In conclusion, the proteome and transcriptome correlation results from this study indicate that ion transport plays a critical role in the adaptation of S. paramamosain to sudden reduction in salinity.

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Hemolymph ammonia and urea and nitrogenous excretions of Scylla serrata at different temperature and salinity levels

Cited by 29 publications

References 19 publications

Acute toxicity of total ammonia to Macrobrachium rosenbergii postlarvae at different salinity levels

Acute toxicity of total ammonia to Macrobrachium rosenbergii postlarvae at different salinity levels

Effect of salinity on survival, growth, oxygen consumption and ammonia-N excretion of juvenile whiteleg shrimp,Litopenaeus vannamei

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

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