Hemolymphatic System

Raskin, Rose E.

doi:10.1016/b978-1-4557-4083-3.00004-8

Cited by 18 publications

(20 citation statements)

References 64 publications

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“…Cytological diagnoses were expressed according to those reported in the literature [4,30,38,39]. To facilitate comparison of the agreement between cytological and histological results, each cytological sample was further classified as neoplastic or non-neoplastic according to the main pathologic process.…”

Section: Methodsmentioning

confidence: 99%

“…To facilitate comparison of the agreement between cytological and histological results, each cytological sample was further classified as neoplastic or non-neoplastic according to the main pathologic process. Non-neoplastic samples were those characterized by degenerative, reactive (including extramedullary hematopoiesis) [23,30], and inflammatory changes, as well as normal specimens consisting of stromal elements with mixed leukocyte population [30,38]. Reviewing cytologists were not allowed to use diagnostic modifiers such as “probably”, “most likely”, “suggestive of”, as previously reported [37], nor to provide equivocal diagnoses (i.e.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

et al. 2019

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Cytology represents a useful diagnostic tool in the preliminary clinical approach to canine splenic lesions, and may prevent unnecessary splenectomy. However, few studies have evaluated diagnostic accuracy of cytology in the diagnosis of canine splenic neoplasms. The aim of this study was to determine overall accuracy, sensitivity, specificity, positive and negative predictive values (i.e. diagnostic accuracy indexes) of cytology for canine splenic neoplasms following Standards for the Reporting of Diagnostic Accuracy Studies (STARD) guidelines. A consecutive series of canine splenic cytological samples was retrospectively retrieved from the database of the Diagnostic Pathology Service of the Department of Veterinary Medicine (DIMEVET—University of Milan). Histopathology was set as the diagnostic reference standard. Cytological cases were enrolled when slides were available for review and when the same lesion was submitted for histopathology. Seventy-eight (78) lesions were included in the study. By histopathology, 56 were neoplastic and 22 were non-neoplastic. Cytology had an overall accuracy of 73.08% (95% C.I. 61.84%-82.50%), sensitivity of 64.29% (95% C.I. 50.36%-76.64%), specificity of 95.45% (95% C.I. 77.16%-99.88%), and positive and negative predictive values of 97.3% (95% C.I. 84.01%-99.60%) and 51.22% (95% C.I. 42.21%-60.15%), respectively. Low sensitivity and negative predictive value were balanced by very high specificity and positive predictive value. When positive for neoplasia, cytology represents a useful diagnostic tool to rule in splenic neoplasia, prompting surgery independently from other diagnostic tests. Conversely, a negative cytological result requires additional investigations to confirm the dog to be disease free.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

et al. 2019

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“…1E). A nuclear staining pattern for CD79␣ is known to represent an unspecific reaction in several species including dogs and cats (Raskin, 2016), thus only a distinct cytoplasmic and membranous staining was judged as specific immunoreactivity (Fig. 1H).…”

Section: B Cellsmentioning

confidence: 99%

Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids

Uhde

Lehmbecker

Baumgärtner

et al. 2017

Veterinary Immunology and Immunopathology

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Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical markers are highly needed in order to phenotype distinct immune cells populations in camelids. So far, specific immunohistochemical markers for camelid immune cells are rarely commercially available, and cross-reactivity studies are restricted to the use of frozen dromedary tissues. To bridge this gap, 14 commercially available primary antibodies were tested for their suitability to demonstrate immune cell populations on formalin fixed paraffin-embedded (FFPE) tissue sections of dromedaries, Bactrian camels, llamas, and alpacas in the present study. Out of these, 9 antibodies directed against CD3, CD20, CD79α, HLA-DR, Iba-1, myeloid/histiocyte antigen, CD204, CD208, and CD68 antigen exhibited distinct immunoreaction patterns to certain camelid immune cell subsets. The distribution of these antigens was comparatively evaluated in different anatomical compartments of thymus, spleen, mesenteric, and tracheobronchial lymph nodes. The presented results will provide a basis for further investigations in camelids, especially with respect to the role of the immune response in certain infectious diseases, which harbor a considerable risk to spill over to other species.

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“…Lymphadenitis is defined as an infiltration of one or more non‐lymphoid inflammatory cells into a lymph node (Teske ). Neutrophilic lymphadenitis, also called purulent or suppurative lymphadenitis, is characterised by a neutrophil population exceeding 5% of the cellular population within a lymph node (Raskin ). It may be associated with bacterial, neoplastic or immune‐mediated diseases.…”

Section: Introductionmentioning

confidence: 99%

Canine sterile steroid‐responsive lymphadenitis in 49 dogs

Latre¹,

McPartland

Cain

et al. 2019

J of Small Animal Practice

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Objectives To report clinical and laboratory features, treatment responses and outcome in dogs diagnosed with sterile steroid‐responsive lymphadenitis in the United Kingdom. Materials and Methods Medical records of dogs diagnosed with sterile steroid‐responsive lymphadenitis from 2009 to 2016 at six specialist referral centres were evaluated retrospectively. Results The study included 49 dogs. Springer spaniels appeared to be over‐represented (16 of 49 dogs). Young dogs (median age: 3 years and 9 months) and females (31 of 49) were frequently affected. Clinical presentation was variable, with pyrexia (39 of 49), lethargy (35 of 49) and anorexia (21 of 49) the most commonly reported clinical signs. Lymph node cytology or histopathology demonstrated neutrophilic, pyogranulomatous, granulomatous or necrotising lymphadenitis without a detectable underlying cause in all cases. Because a sterile immune‐mediated aetiology was suspected, all dogs received prednisolone, which was followed by rapid resolution of clinical signs and lymphadenopathy in most cases. Clinical Significance Sterile steroid‐responsive lymphadenitis should be considered in dogs with pyrexia of unknown origin with inflammatory lymphadenopathy if no underlying cause can be found and often responds well to immunosuppressive corticosteroid therapy.

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Hemolymphatic System

Cited by 18 publications

References 64 publications

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms: An investigation in 78 cases using STARD guidelines

Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids

Canine sterile steroid‐responsive lymphadenitis in 49 dogs

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