Hemolytic activity of new linear surfactin analogs in relation to their physico-chemical properties

Dufour, Samuel; Deleu, Magali; Nott, Kathérine; Wathelet, Bernard; Thonart, Philippe; Paquot, Michel

doi:10.1016/j.bbagen.2005.06.015

Cited by 104 publications

(96 citation statements)

References 37 publications

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“…It has been shown that surfactin penetration is promoted by longer lipopeptide chain length (Eeman et al, 2006). The data suggested that particularly high hydrophobic lipopeptides have an increased cytotoxic activity on cells (Deleu et al, 2003;Dufour et al, 2005). Furthermore, the significant difference of cytotoxicity in Fig.…”

Section: Discussionmentioning

confidence: 89%

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Tao

Zou

et al. 2010

Protein Cell

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Microbial lipopeptides play an important role in apoptosis induction of tumor cells. However, there is little knowledge about the relationship between apoptosis induction and membrane fatty acids. The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl (MTT) colorimetric assay and surface tension measurements, showed that the critical micelle concentration (CMC) was a critical level for the inhibitory activity of lipopeptides on the growth of Bcap-37 cells. Under the CMC, the order of least to greatest cytotoxicity effect on cancer cell lines by lipopeptides is C 13 -lipopeptide < C 14 -lipopepitde < C 15 -lipopeptide. Above CMC, all lipopeptides directly exert cytolytic activity. The flow cytometric analysis and Hoechst33258 staining experiments confirmed the apoptosis of Bcap-37 cell lines induced by lipopeptides in a dose-dependent manner. This apoptosis was associated with a significant decrease of the unsaturated degree of the cellular fatty acids of Bcap-37 cell lines due to the changes in the cellular fatty acids composition induced by the lipopeptide treatment. These results indicated that disturbance of the cellular fatty acid composition of breast cancer cell lines were related to in the cell apoptosis. Furthermore, significant difference in IC 50 values of tumor cells and normal cell showed that the lipopeptide exerted selective cytotoxicity on the cancer cells. Thus HSO121 lipopeptides may have potential applications as an anticancer leads.

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Section: Discussionmentioning

confidence: 89%

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Tao

Zou

et al. 2010

Protein Cell

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“…Deuterium NMR has revealed that the hydrophobic residues of the peptide moiety penetrate rather deeply into the membrane interface, where they may induce a stronger destabilization than typical detergents (Heerklotz et al 2004). Dufour et al (2005) have shown by comparing cyclic with linear surfactin analogs that the bulky nature of the cyclic peptide group is a prerequisite for membrane permeabilization but not for surface activity, as expected for detergentlike behaviour. The proposal that membranes are permeabilized by dimers of surfactin (Carrillo et al 2003) argues, however, in favor of a more specific mechanism.…”

Section: Introductionmentioning

confidence: 99%

Leakage and lysis of lipid membranes induced by the lipopeptide surfactin

2006

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Surfactin is a lipopeptide produced by Bacillus subtilis which possesses antimicrobial activity. We have studied the leakage and lysis of POPC vesicles induced by surfactin using calcein fluorescence de-quenching, isothermal titration calorimetry and 31 P solid state NMR. Membrane leakage starts at a surfactin-to-lipid ratio in the membrane, R b % 0.05, and an aqueous surfactin concentration of C S w % 2 lM. The transient, graded nature of leakage and the apparent coupling with surfactin translocation to the inner leaflet of the vesicles, suggests that this low-concentration effect is due to a bilayer-couple mechanism. Different permeabilization behaviour is found at R b % 0.15 and attributed to surfactin-rich clusters, which can induce leaks and stabilize them by covering their hydrophobic edges. Membrane lysis or solubilization to micellar structures starts at R b sat = 0.22 and C S w = 9 lM and is completed at R m sol = 0.43 and C S w = 11 lM. The membrane-water partition coefficient of surfactin is obtained as K = 2 · 10 4 M -1 . These data resolve inconsistencies in the literature and shed light on the variety of effects often referred to as detergent-like effects of antibiotic peptides on membranes. The results are compared with published parameters characterizing the hemolytic and antibacterial activity.

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“…RBCs were then washed once in PBS-EDTA 0.075 % and three times in an isotonic buffer (PBS). RBCs were then suspended in this buffer at a cell density of 5-108 cells ml -1 (Dufour et al 2005). Then for hemolysis assay, CH-PVA polymeric blend and CH-PVA/HA nano composite membrane dissolved in small portions of distilled water and 1 M HCl (Chen et al 2004) respectively, and for each sample, prepared solutions in 5, 10, 15 and 20 mg ml -1 concentrations.…”

Section: Hemolysis Assaymentioning

confidence: 99%

“…The negative control prepared by adding 100 ml of RBCs suspension to 900 ml of PBS. The RBCs in PBS remained safe (Dufour et al 2005). Each experiment has been performed at least twice.…”

Section: Hemolysis Assaymentioning

confidence: 99%

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

Imanieh¹,

Aghahosseini²

2013

Syst Synth Biol

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Development of bioorganic-inorganic composites has drawn eyes to extensive attention in biomedical fields and tissue engineering. So many attempts to prepare hydroxyapatite (HA), in conjunction with various binders including polyvinyl alcohol (PVA), and collagen has performed for late 20 years. We applied a method based on the phase separation for making of polymer porous membranes. This procedure is induced through the addition of a small quantity of water (polymer-rich phase) to a solution with HA precursors (polymer-poor phase). Thermal and structural composite properties of collagen Hydrolysate (CH)-PVA/HA Polymer-Nano-Porous Membranes were analyzed by Design of experiment that was undertaken using D-optimal approach, to select the optimal combination of nano composites precursor. The resulted composite characters were investigated by Fourier transform infrared, scanning electron microscopy (SEM) and thermal gravimetric analysis. Based on the SEM images, this new method could be clearly concluded to porous CH-PVA/HA hybrid materials. Finally the hemocompatibility of nanocomposite membranes were evaluated by the hemolysis study.

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Hemolytic activity of new linear surfactin analogs in relation to their physico-chemical properties

Cited by 104 publications

References 37 publications

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Leakage and lysis of lipid membranes induced by the lipopeptide surfactin

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

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