Hemostatic action of lotus leaf charcoal is probably due to transformation of flavonol aglycons from flavonol glycosides in traditional Chinses medicine

Chen, Yuhuan; Chen, Qiwen; Wang, Xiaozhong; Sun, Fan; Fan, Yanting; Liu, Xiaoru; Li, Hongyan; Deng, Zeyuan

doi:10.1016/j.jep.2019.112364

Cited by 23 publications

(13 citation statements)

References 32 publications

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“…Such compounds are also found in MAP (Chen et al, 2020). Quercetin, a compound which is also present in MAP (Veeresham et al, 2013) is known to have hemostatic action (Chen et al, 2020). It is evident that the prior incubation of the plasma with venom caused the irreversible destruction of clotting factors of the intrinsic and common pathway as observed Fig.…”

Section: Discussionmentioning

confidence: 85%

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Evaluation of the merit of the methanolic extract of Andrographis paniculata to supplement anti-snake venom in reversing secondary hemostatic abnormalities induced by Naja naja venom

et al. 2021

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Increasing evidence suggests a sizable involvement of hemotoxins in the morbidity associated with envenomation by the Indian spectacled cobra, Naja naja (N.N). This study investigates the ability of Indian polyvalent anti-snake venom (ASV), methanolic extract of Andrographis paniculata (MAP) and their combination in reversing the hemostatic abnormalities, viz. activated partial thromboplastin time(aPTT), prothrombin time(PT) and thrombin time(TT) in citrated plasma. These parameters were assessed in 2 groups of experiments. Group 1: Without the prior incubation of plasma with venom and Group 2: With prior incubation of plasma with venom for 90 min at 37°C. Venom caused significant (p < 0.001) prolongation in aPTT (175%), PT (49%) and TT (34%) in Group 1 and ASV could completely bring them back to normal. MAP showed a concentration-dependent reversal in aPTT, normalization of PT and prolongation of TT. When low concentration of ASV was supplemented with MAP, their combined effect in normalizing aPTT and PT improved by 37% and 26% respectively when compared to ASV alone. In Group 2, venom caused significant (p < 0.001) prolongation in aPTT (231%), PT (312%) and TT (245%). ASV had limited effect in reversing aPTT (52%), TT (31%) but completely normalized PT. MAP was marginally effective in reversing the prolonged aPTT and PT but caused further prolongation of TT. Combination of ASV and MAP was more effective than ASV alone in reversing venom-induced increase in aPTT (52%) and PT (29%). The study proved that, a drastic reduction of ASV by 70%, could be effectively supplemented by MAP in combating hemostatic abnormalities induced by NN venom.

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Section: Discussionmentioning

confidence: 85%

“…These constituents are known to have pro-coagulant property (Liang et al, 2019). Such compounds are also found in MAP (Chen et al, 2020). Quercetin, a compound which is also present in MAP (Veeresham et al, 2013) is known to have hemostatic action (Chen et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the merit of the methanolic extract of Andrographis paniculata to supplement anti-snake venom in reversing secondary hemostatic abnormalities induced by Naja naja venom

et al. 2021

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“…One study [6] showed that tannins play a major role in the haemostatic effect of carbon drugs. Another study reported that it was a change in flavonoids that was closely related to haemostatic effect enhancement [7]. These inconsistent results cannot reasonably explain the similar haemostatic effects of many different charcoal medicines.…”

Section: Introductionmentioning

confidence: 99%

Haemostatic Nanoparticles-Derived Bioactivity of from Selaginella tamariscina Carbonisata

et al. 2020

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High-temperature carbonisation is used to prepare many traditional Chinese medicine charcoal drugs, but the bioactive haemostatic substances of these medicines and their mechanisms are still unknown. This study developed and evaluated nanoparticles (NPs) derived from Selaginella pulvinate Carbonisata (STC) for the first time. The haemostatic effect of STC-NPs prepared at 300, 350, and 400 °C were investigated in mouse tail amputation and liver scratch experiments. STC-NPs obtained at 400 °C had the strongest haemostatic effect, and were accordingly characterised by ultraviolet–visible spectroscopy, fluorescence spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, high resolution transmission electron microscopy, X-ray diffractometry, and X-ray photoelectron spectroscopy. STC-NPs averaged 1.4–2.8 nm and exhibited a quantum yield of 6.06% at a maximum excitation wavelength of 332 nm and emission at 432 nm. STC-NPs displayed low toxicity against mouse monocyte macrophage RAW 264.7 cells by CCK-8 assay, and STC-NP treatment significantly shortened bleeding time in rat and mouse models. Coagulation assays showed that the haemostatic effects of STC-NPs were related to improving the fibrinogen and platelet contents, as well as decreasing the prothrombin time that resulted from stimulating extrinsic blood coagulation and activating the fibrinogen system. The STC-NPs had remarkable haemostatic effects in the tail amputation and liver scratch models; these effects may be associated with the exogenous coagulation pathway and activation of the brinogen system, according to the evaluation of the mouse coagulation parameters. This novel evaluation supports the material basis of STC use in traditional Chinese medicine, and this article is worthy of study by authors of clinical pharmacy.

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“…Researchers determined the flavonoid aglycones and flavonoid glycosides contents. The ethyl acetate extracts (flavonoid glycosides, 140.61 ± 5.26 mg/g; flavonoid aglycones, 62.26 ± 2.40 mg/g) could significantly reduce blood clotting time ( Chen et al, 2020 ). Tannins ( Cao et al, 2010 ), total flavonoids ( Zhang et al, 2005 ) and CDs ( Zhang et al, 2018 ) are active fractions with hemostasis in Nepeta tenuifolia Benth.…”

Section: Introductionmentioning

confidence: 99%

Chinese Medicinal Herb-Derived Carbon Dots for Common Diseases: Efficacies and Potential Mechanisms

Zhao

et al. 2022

Front. Pharmacol.

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The management of hemorrhagic diseases and other commonly refractory diseases (including gout, inflammatory diseases, cancer, pain of various forms and causes) are very challenging in clinical practice. Charcoal medicine is a frequently used complementary and alternative drug therapy for hemorrhagic diseases. However, studies (other than those assessing effects on hemostasis) on charcoal-processed medicines are limited. Carbon dots (CDs) are quasi-spherical nanoparticles that are biocompatible and have high stability, low toxicity, unique optical properties. Currently, there are various studies carried out to evaluate their efficacy and safety. The exploration of using traditional Chinese medicine (TCM) -based CDs for the treatment of common diseases has received great attention. This review summarizes the literatures on medicinal herbs-derived CDs for the treatment of the difficult-to-treat diseases, and explored the possible mechanisms involved in the process of treatment.

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Hemostatic action of lotus leaf charcoal is probably due to transformation of flavonol aglycons from flavonol glycosides in traditional Chinses medicine

Cited by 23 publications

References 32 publications

Evaluation of the merit of the methanolic extract of Andrographis paniculata to supplement anti-snake venom in reversing secondary hemostatic abnormalities induced by Naja naja venom

Evaluation of the merit of the methanolic extract of Andrographis paniculata to supplement anti-snake venom in reversing secondary hemostatic abnormalities induced by Naja naja venom

Haemostatic Nanoparticles-Derived Bioactivity of from Selaginella tamariscina Carbonisata

Chinese Medicinal Herb-Derived Carbon Dots for Common Diseases: Efficacies and Potential Mechanisms

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