Hemostatic activities of methanol leaf extracts of Croton megalocarpus Hutch and Lantana camara Linn

Muindi, Hezron Mutisya; Kibiti, Cromwell Mwiti; Ngugi, Mathew Piero

doi:10.5455/jabet.2021.d122

Cited by 2 publications

(2 citation statements)

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“…Medicinal plants have been used for many decades to treat gastrointestinal helminthiasis, and there is currently a growing demand for plant-derived anthelmintic compounds due to their potency, efficacy, and reduced side effects. 11,5,18 In the anthelmintic assay, we observed that the fractions of F. indica exhibited moderate anthelmintic properties. The different solvent fractions caused both the loosening of motion and death of the P.…”

Section: Discussionmentioning

confidence: 90%

Exploration of Anthelmintic, Blood Coagulant, Diuretic and Laxative Activities of Different Solvent Fractions of Flagellaria Indica Leaves

Karmakar,

Paul,

Kundu

et al. 2023

Jordan j. pharm. sci.

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Flagellaria indica (Family: Flagellariaceae) is a common climbing plant found primarily in tropical regions of many countries. The plant has various traditional uses, although most of them lack scientific published reports. The crude ethanolic extract of F. indica leaves was fractionated based on polarity using water, ethyl acetate, and n-hexane. Biological screening was conducted on the anthelmintic, blood coagulation, diuretic, and laxative activities of the water, ethyl acetate, and n-hexane fractions of F. indica leaves. In the anthelmintic test, the n-hexane fraction showed a moderate effect with paralysis times of 16.79 and 13.62 minutes and death times of 27.34 and 21.81 minutes, respectively, at doses of 25 and 50 mg/mL.In the blood coagulant test, only the water fraction showed a notable effect. The clotting times were 4.33, 6.02, 7.68, and 8.32 minutes, respectively, at doses of 200, 100, 50, and 25 mg/mL. Diuretic activity was performed to determine the increase in the volume of excreted urine, and electrolyte analysis of urine was performed to determine pH, density, conductance, and Na+, Cl-, K+ levels, as well as natriuretic, kaliuretic, saluretic, and CAI indexes. The ethyl acetate fraction showed better diuretic activity than the n-hexane fraction, while the water fraction did not reveal a notable diuretic effect. The Na+, Cl-, K+, natriuretic, and saluretic indexes were found satisfactory in the ethyl acetate fraction, and the CAI index was better in the n-hexane fraction. In the laxative test, the n-hexane fraction showed the best laxative properties, with an increase in stool weight of 38% and 54% at doses of 250 and 500 mg/kg, respectively. These results suggest that different fractions of F. indica leaves contain distinct phytochemicals that may be responsible for these biological effects. The isolation of bioactive compounds could help justify its traditional uses in modern medicine.

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Section: Discussionmentioning

confidence: 90%

Exploration of Anthelmintic, Blood Coagulant, Diuretic and Laxative Activities of Different Solvent Fractions of Flagellaria Indica Leaves

Karmakar,

Paul,

Kundu

et al. 2023

Jordan j. pharm. sci.

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“…Four compounds had two carboxylic and alcoholic groups and appear to interact with coagulation factors with lower energy. In the studies conducted by Muindi et al to investigate the effects of methanolic extracts of Croton megalocarpus and Lantana camara plants, the compound quercetin-3-O-B-D-glucoside decreased the activated partial thromboplastin time (APTT) (100, 101). Lin et al investigated the effects of Sedum aizoon compounds on the coagulation system; in this study, Gallic acid (102) caused a reduction in the time of internal blood coagulation in vivo and in vitro, having the same carboxylic acid functional group as our study compounds (102).…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Potential Blood Coagulant Activity ofCaenothus americanus Compounds: Computational Analysis using Docking, Physicochemical, and ADMET Studies

Mashkani,

Yali,

Dorgalaleh

et al. 2023

Preprint

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Caenothus americanus is a common folk remedy for the treatment of wound bleeding. Certain compounds found in this plant have been shown to reduce clotting time. However, analyzing the effects of various compounds of a folk remedy is a time-consuming and expensive process, therefore, this study employed computational analyses using docking, physicochemical, and pharmacokinetic servers to identify potential clotting activity in C.americanus compounds. The ADMETlab, SwissADME web servers, Discovery Studio, and Autodock were used to study the proper binding to target proteins and predict the physicochemical and ADMET properties (adsorption, distribution, metabolism, excretion, and toxicity) of C. americanus compounds. Coagulation factors including activated factor (F) IIa, FVa, FVIIa, FVIIIa, FIXa, FXa, FXIa, FXIIa, and FXIIIa were chosen as target proteins. Docking studies revealed that malic acid, malonic acid, oxalic acid, and succinic acid were effective on coagulation factors, of which, malic acid had better binding to intrinsic pathway factors including FVIIa, FIIa, and FXIIIa (except FVIIIa), oxalic acid to FVIIIa, and malonic acid to FVa and FXa. Moreover, ADMET studies showed the safety profile of these compounds. In conclusion, carboxylic and alcoholic groups of malic acid, malonic acid, oxalic acid and succinic acid play a role in interaction with blood coagulation factors. Additionally, based on the ADMET characteristics and suitable pharmacokinetic potentials of these compounds, they can be introduced as blood coagulant candidates with fewer side effects in bleeding disorders. However, further studies are necessary to evaluate the precise components of the C. americanus with the suability to bind coagulation factors. Keywords: Caenothus americanus, molecular docking, coagulation factors, plant, ADME.

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Hemostatic activities of methanol leaf extracts of Croton megalocarpus Hutch and Lantana camara Linn

Cited by 2 publications

References 0 publications

Exploration of Anthelmintic, Blood Coagulant, Diuretic and Laxative Activities of Different Solvent Fractions of Flagellaria Indica Leaves

Exploration of Anthelmintic, Blood Coagulant, Diuretic and Laxative Activities of Different Solvent Fractions of Flagellaria Indica Leaves

Evaluating the Potential Blood Coagulant Activity ofCaenothus americanus Compounds: Computational Analysis using Docking, Physicochemical, and ADMET Studies

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