Hemostatic chemical constituents from natural medicine Toddalia asiatica root bark by LC-ESI Q-TOF MSE

Abstract

Background Toddalia asiatica root bark as an effective hemostatic natural medicine or Chinese materia medica was applied in China for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug.ResultsAn intelligentized strategy by LC-ESI Q-TOF MSE was presented in this study for rapid identification of hemostatic chemical constituents from this natural medicine. Chromatographic separation was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm), the MSE data in both negative and positive ion modes were acquired to record the high-accuracy MS and MS/MS data of all precursor ions. To reduce the false positive identifications, structural confirmation was conducted by comparison with the isolated reference standards (tR and MS, MS/MS data) or matching with natural product databases. Bioassay-guided fractionation of the extract of T. asiatica root bark was also carried out.ConclusionsAs a consequence, 31 natural compounds in T. asiatica root bark got putatively characterized. There were four main coumarins, isopimpinellin (Cp.23), pimpinellin (Cp.24), coumurrayin (Cp.30) and phellopterin (Cp.34) isolated and identified from T. asiatica root bark. The present study provided candidate strategy that helps to effectively identify the primary natural compounds of TCM or other complex natural medicines, and then promote development and application of natural medicines and their medicinal resources.

Development of a method to screen and isolate bioactive constituents from Stellera chamaejasme by ultrafiltration and liquid chromatography combined with semi‐preparative high‐performance liquid chromatography and high‐speed counter current chromatography

“…This was evident in the secondary mass spectra (fragment ions at m/z 232.04, 203.03, and 187.04). The molecular weight of compound 2 was consistent with that reported previously for isopimpinellin .…”

Separation of New Coumarin Glycosides from Toddalia asiatica Using Offline Two-Dimensional High-Performance Liquid Chromatography

“…(Rutaceae) (Syn: Toddalia aculeata Pers., Scopolia aculeata Sm., Paullinia asiatica L.), well known as Feilongzhangxue in Chinese and commonly known as orange climber in English, is widely distributed in the east, south, and southeast of Asia, Madagascar, Africa, and the Mascarene Islands [1,2]. The roots and barks of T. asiatica have been used in Miao minority medicine, mainly in Guizhou, Yunnan, and Guangxi provinces in China, for the treatment of fall injuries, rheumatic arthralgia, stomachache, chronic lumbago, and diarrhea [3,4]. Previous phytochemical and pharmacological investigations of different parts of this plant revealed that coumarins are the main secondary metabolites [5], some of which exhibited cytotoxic, antimalarial [6], antiviral [7], anti-inflammatory [8], antibacterial [9], anti-platelet aggregation [10], anti-plasmodial, larvicidal [11], phosphodiesterase-4 inhibitory [12], and osteoclastogenesis activities [13].…”

Rapid discrimination of Notopterygium incisum and Notopterygium franchetii based on characteristic compound profiles detected by UHPLC‐QTOF‐MS/MS coupled with multivariate analysis

“…Ten compounds were unambiguously identified as chlorogenic acid, ferulic acid, nodakenin, bergapten, 3,4‐dicaffeoylquinic acid, 3,5‐dicaffeoylquinic acid, 4,5‐dicaffeoylquinic acid, imperatorin, notopterol and isoimperatorin (peaks 6, 16, 17, 18, 21, 22, 34, 38, 42 and 44, respectively) based on the retention time and mass spectra of these standards. The identification of other compounds was deduced by reference to previously published data, performing accurate m / z value analysis of the TOF‐MS data, and with QTOF‐MS/MS fragment analysis. All detected compounds are shown in Table .…”