Hemostatic Effects of A-fact, which is a new hemostatic agent

Abstract

A-Fact is a solution contains iron (II) sulfate and forms an iron-ion-protein complex. An ideal hemostatic agent should have been stopping venous bleeding after being applied to the lesion. Our aim in the present study is to investigate the in vitro effects of A-Fact on hemostatic parameters. Method: Plasma pooling was performed by centrifuging venous blood samples from volunteer people. Total protein, fibrinogen activity, albumin and globulin, coagulation factor (II, VII, IX) levels were analyzed with spectrophotometric method in auto analyzer by applying different concentrations of A-Fact. Microscopic images were obtained by applying 5%, 10%, 15% and 50% A-fact to blood samples for morphological evaluation. Results: A-Fact ensured the formation of the iron-ion-protein complex. Coagulation tests were performed by 2/20 dilution. A-Fact was not effect on coagulation factors. Plasma fibrinogen activity, total protein, albumin and globulin levels were decreased while prothrombin time was prolonged. Furthermore, it was observed that A-Fact caused an increase in INR values. Conclusions: In this study, it was showed that the bleeding-stopping effect of A-Fact may be caused by protein formation. It is clear that further studies are needed in this area.