Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress

Wen, Zheng-Shun; Xue, Ran; Du, Ming; Tang, Zhen; Xiang, Xingwei; Zheng, Bin; Qu, Youle

doi:10.1016/j.ijbiomac.2019.05.082

Cited by 43 publications

(33 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contradictory with the increase in the nuclear NRF2 accumulation, the mRNA expression of NQO1 and SOD1 was downregulated in IPEC-1 cells during oxidative stress, and this may be due to cell antioxidant system failure. A similar result has also been reported recently, in which H 2 O 2 upregulates cellular NRF2 protein levels but downregulates the mRNA expression of SOD1 in IPEC-1 cells [50]. Studies have demonstrated that fucoidan prevents oxidative damage by activating the NRF2 signaling pathway in human hepatocytes [22] and human lung fibroblast cells [20].…”

Section: Discussionsupporting

confidence: 84%

Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells

Zhao

Wang

et al. 2019

Animals

View full text Add to dashboard Cite

This study was conducted to evaluate the effectiveness of fucoidan in ameliorating hydrogen peroxide (H2O2)-induced oxidative stress to porcine intestinal epithelial cell line (IPEC-1). The cell viability test was initially performed to screen out appropriate concentrations of H2O2 and fucoidan. After that, cells were exposed to H2O2 in the presence or absence of pre-incubation with fucoidan. Hydrogen peroxide increased the apoptotic and necrotic rate, boosted reactive oxygen species (ROS) generation, and disturbed the transcriptional expression of genes associated with antioxidant defense and apoptosis in IPEC-1 cells. Pre-incubation with fucoidan inhibited the increases in necrosis and ROS accumulation induced by H2O2. Consistently, in the H2O2-treated IPEC-1 cells, fucoidan normalized the content of reduced glutathione as well as the mRNA abundance of NAD(P)H quinone dehydrogenase 1 and superoxide dismutase 1 while it prevented the overproduction of malondialdehyde. Moreover, H2O2 stimulated the translocation of nuclear factor-erythroid 2-related factor-2 to the nucleus of IPEC-1 cells, but this increase was further promoted by fucoidan pre-treatment. The results suggest that fucoidan is effective in protecting IPEC-1 cells against oxidative damage induced by H2O2, which may help in developing appropriate strategies for maintaining the intestinal health of young piglets.

show abstract

Section: Discussionsupporting

confidence: 84%

Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells

Zhao

Wang

et al. 2019

Animals

View full text Add to dashboard Cite

show abstract

“…Molecular and structural analyses of the Nrf2 pathway revealed a “dedepression” regulatory mechanism, wherein Nrf2 is anchored in the cytoplasm via Keap1-dependent ubiquitination-proteasomal degradation under normal physiological conditions in order to maintain antioxidant and cytoprotective enzymes at basal level and the cells in a stable state [ 30 ]. The Nrf2 pathway is stimulated by electrophiles and oxidants through the modification of critical cysteine thiols of Keap1 and/or phosphorylation of Nrf2, and the subsequently enhanced expression of antioxidant enzymes is achieved from the releasing of Nrf2 from Keap1 and translocating to the nucleus [ 31 ]. In the current study, protein expressions of both Nrf2 and Keap1 decreased after being treated with LPS, which was consistent with previous studies using different doses of pro-oxidants [ 7 , 20 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Dandelion Extract Alleviated Lipopolysaccharide-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells

Sun

Wang

et al. 2020

Toxins

View full text Add to dashboard Cite

In practical dairy production, cows are frequently subjected to inflammatory diseases, such as high-grain diet-induced subacute ruminal acidosis (SARA) as well as mastitis and metritis. Under the circumstances, lipopolysaccharide (LPS) induces oxidative stress within the cow and in the mammary epithelial cells. It has implications in practical production to alleviate oxidative stress and to optimize the lactational function of the mammary epithelial cells. This study thus aimed to investigate the antioxidative effects of dandelion aqueous extract (DAE) on LPS-induced oxidative stress and the mechanism of DAE as an antioxidant to alleviate oxidative stress through the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in the bovine mammary epithelial cell line MAC-T cells. The cells were cultured for 48 h in six treatments including control (without LPS and DAE), LPS (100 ng/mL), DAE10 (100 ng/mL LPS and 10 μg/mL DAE), DAE50 (100 ng/mL LPS and 50 μg/mL DAE), DAE100 (100 ng/mL LPS and 100 μg/mL DAE), and DAE200 (100 ng/mL LPS and 200 μg/mL DAE), respectively. The results showed that cell viability was reduced by LPS, and the adverse effect of LPS was suppressed with the supplementation of DAE. Lipopolysaccharide-induced oxidative stress through enhancing reactive oxygen species (ROS) production, resulted in increases in oxidative damage marker concentrations, while 10 and 50 μg/mL DAE alleviated the LPS-induced oxidative stress via scavenging cellular ROS and improving antioxidant enzyme activity. The upregulation of antioxidative gene expression in DAE treatments was promoted through activating the Nrf2 signaling pathway, with DAE at a concentration of 50 μg/mL exhibiting the highest effect. Overall, DAE acted as an effective antioxidant to inhibit LPS-induced oxidative stress and as a potential inducer of the Nrf2 signaling pathway.

show abstract

“…The monosaccharide composition of HSP was determined according to the precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) high-performance liquid chromatography method [ 24 ]. The sulfate contents of HSP sample was determined according to a published literature [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The primer sequences were obtained from Shanghai Shenggong Biotechnology Co., Ltd. (Shanghai, China) and listed in Table 2 . The relative mRNA expression of the target gene was measured as described previously [ 21 ]. The target genes mRNA expression were analyzed by the 2 - ΔΔ Ct method and normalized to the mean values of internal reference gene ( β -actin).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies reported that cold-pressed hemp seed oil had strong free radical scavenging activity and oxidative stability in vitro [ 20 ]. Moreover, our previous study found the polysaccharide of hemp seed (HSP, a sulfated polysaccharide) displayed good antioxidant activity in vitro [ 21 ]. Investigations on hemp seed suggest that it has potential as an alternative treatment for oxidative stress-related diseases.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polysaccharides from Hemp Seed Protect against Cyclophosphamide-Induced Intestinal Oxidative Damage via Nrf2-Keap1 Signaling Pathway in Mice

Xue

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Hemp seed has been used as a traditional oriental medicine and health food in China for centuries. Polysaccharides from hemp seed (HSP) exhibit important properties of intestinal protection, but there are limited data on the specific underlying mechanism. The primary objective of this study was to investigate the protective effect of HSP on intestinal oxidative damage induced by cyclophosphamide (Cy) in mice. The results showed that pretreatment with HSP significantly increased the average daily gain, thymus index, spleen index, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity in serum and ileal homogenate and significantly reduced malondialdehyde (MDA) content in ileal homogenate. In addition, the expression levels of SOD, GSH-Px, Nrf2, heme oxidase-1 (HO-1), and quinoneoxidoreductase-1 (NQO1) mRNA in ileal homogenate were significantly increased. Western blot results showed that HSP significantly upregulated the expression of Nrf2 protein and downregulated the expression of Keap1 protein in the ileum. Collectively, our findings indicated that HSP had protective effects on intestinal oxidative damage induced by Cy in mice, and its mechanism might be related to the activation of Nrf2-Keap1 signaling pathway.

show abstract

Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress

Cited by 43 publications

References 37 publications

Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells

Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells

Dandelion Extract Alleviated Lipopolysaccharide-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells

Polysaccharides from Hemp Seed Protect against Cyclophosphamide-Induced Intestinal Oxidative Damage via Nrf2-Keap1 Signaling Pathway in Mice

Contact Info

Product

Resources

About