Heparin Inhibits Contraction of Smooth Muscle Cells in Lymphatic Vessels

Lobov, G. I.; Pan’kova, M. N.

doi:10.1007/s10517-010-0860-0

Cited by 12 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was also shown that heparin modulated lymph vessel contractions by stimulating NO production by endotheliocytes [1]. In the present study, addition of L-NAME (NO synthase blocker) to bathing solution 20 min before heparin led to a signifi cant attenuation of the relaxing effects of heparin.…”

Section: Resultssupporting

confidence: 57%

“…The NO in smooth muscle cell cytoplasm of LN capsules, similarly as in the myocytes of blood and lymph vessels, activates soluble guanylate cyclase, which leads to an increase in cGMP concentration with subsequent activation of ATP + K + channels and development of SM relaxation responses [1,5,10]. Preliminary addition of methylene blue (n=8) or glibenclamide (n=11) into bathing solution attenuated the relaxing effects of heparin (Fig.…”

Section: Resultsmentioning

confidence: 96%

See 1 more Smart Citation

Effect of Heparin on Contractile Activity of Lymph Node Capsule

Pan’kova

Lobov

2015

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

Experiments on smooth muscle strips isolated from the capsule of bovine mesenteric lymph node showed that heparin low concentrations (0.010-0.025 U/ml) stimulating spontaneous isometric phasic contractions of smooth muscles, but higher heparin concentrations reduced or even completely eliminated these contractions. The inhibitory effects of heparin were presumably realized via stimulation of NO production by endothelial cells of the subcapsular space followed by activation of ATP-sensitive potassium channels of capsular myocytes and via modulation of prostaglandin synthesis.

show abstract

Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 96%

Effect of Heparin on Contractile Activity of Lymph Node Capsule

Pan’kova

Lobov

2015

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, there may be differences in the extent of lymphangion regional variation between mesenteric lymphatics and larger collecting ducts (e.g., thoracic duct) and even more variation between species. For example, Lobov 27 described a more rigid valvular portion in bovine mesenteric lymphatic vessels. We believe that his results are attributable to both the larger vessel diameter and the greater smooth muscle component of those lymphatic vessels.…”

Section: Discussionmentioning

confidence: 99%

Passive Pressure–Diameter Relationship and Structural Composition of Rat Mesenteric Lymphangions

Rahbar

Weimer

Gibbs

et al. 2012

Lymphatic Research and Biology

View full text Add to dashboard Cite

Background: Lymph flow depends on both the rate of lymph production by tissues and the extent of passive and active pumping. Here we aim to characterize the passive mechanical properties of a lymphangion in both midlymphangion and valve segments to assess regional differences along a lymphangion, as well as evaluating its structural composition. Methods and Results: Mesenteric lymphatic vessels were isolated and cannulated in a microchamber for pressure-diameter (P-D) testing. Vessels were inflated from 0 to 20 cmH 2 O at a rate of 4 cmH 2 O/min, and vessel diameter was continuously tracked, using an inverted microscope, video camera, and custom LabVIEW program, at both mid-lymphangion and valve segments. Isolated lymphatic vessels were also pressure-fixed at 2 and 7 cmH 2 O and imaged using a nonlinear optical microscope (NLOM) to obtain collagen and elastin structural information. We observed a highly nonlinear P-D response at low pressures (3-5 cmH 2 O), which was modeled using a three-parameter constitutive equation. No significant difference in the passive P-D response was observed between mid-lymphangion and valve regions. NLOM imaging revealed an inner elastin layer and outer collagen layer at all locations. Lymphatic valve leaflets were predominantly elastin with thick axially oriented collagen bands at the insertion points. Conclusions: We observed a highly nonlinear P-D response at low pressures (3-5 cmH 2 O) and developed the first constitutive equation to describe the passive P-D response for a lymphangion. The passive P-D response did not vary among regions, in agreement with the composition of elastin and collagen in the lymphatic wall.

show abstract

“…In particular it has been shown that histamine is a potent dosedependent modulator of lymphatic contractility (16 -18, 33, 49, 54 -57, 59, 70, 71). Other mast cell-derived mediators like heparin (40,55), serotonin (15,33,54,70), leukotrienes B4 and C4 (17,33), and thromboxane A 2 (59,65) are also shown to have physiological effects on lymphatic contractility. However, until now, in only one study (59) the activation and degranulation of mast cells has been directly linked to subsequent changes in lymphatic contractility mediated by histamine.…”

mentioning

confidence: 99%

Aging-associated shifts in functional status of mast cells located by adult and aged mesenteric lymphatic vessels

Chatterjee

Gashev

2012

American Journal of Physiology-Heart and Circulatory Physiology

107

View full text Add to dashboard Cite

We had previously proposed the presence of permanent stimulatory influences in the tissue microenvironment surrounding the aged mesenteric lymphatic vessels (MLV), which influence aged lymphatic function. In this study, we performed immunohistochemical labeling of proteins known to be present in mast cells (mast cell tryptase, c-kit, prostaglandin D(2) synthase, histidine decarboxylase, histamine, transmembrane protein 16A, and TNF-α) with double verification of mast cells in the same segment of rat mesentery containing MLV by labeling with Alexa Fluor 488-conjugated avidin followed by toluidine blue staining. Additionally, we evaluated the aging-associated changes in the number of mast cells located by MLV and in their functional status by inducing mast cell activation by various activators (substance P; anti-rat DNP Immunoglobulin E; peptidoglycan from Staphyloccus aureus and compound 48/80) in the presence of ruthenium red followed by subsequent staining by toluidine blue. We found that there was a 27% aging-associated increase in the total number of mast cells, with an ∼400% increase in the number of activated mast cells in aged mesenteric tissue in resting conditions with diminished ability of mast cells to be newly activated in the presence of inflammatory or chemical stimuli. We conclude that higher degree of preactivation of mast cells in aged mesenteric tissue is important for development of aging-associated impairment of function of mesenteric lymphatic vessels. The limited number of intact aged mast cells located close to the mesenteric lymphatic compartments to react to the presence of acute stimuli may be considered contributory to the aging-associated deteriorations in immune response.

show abstract

Heparin Inhibits Contraction of Smooth Muscle Cells in Lymphatic Vessels

Cited by 12 publications

References 9 publications

Effect of Heparin on Contractile Activity of Lymph Node Capsule

Effect of Heparin on Contractile Activity of Lymph Node Capsule

Passive Pressure–Diameter Relationship and Structural Composition of Rat Mesenteric Lymphangions

Aging-associated shifts in functional status of mast cells located by adult and aged mesenteric lymphatic vessels

Contact Info

Product

Resources

About