Heparinized anticoagulant coatings based on polyphenol-amine inspired chemistry for blood-contacting catheters

Abstract

We offered a strategy for constructing a polyphenol-amine coating with TA and lysine for efficient heparin immobilization. The coated substrates showed good anticoagulant properties and biocompatibility.

The polydopamine‐assisted heparin anchor enhances the hydrophilicity, hemocompatibility, and biocompatibility of polyurethane

“…The functional group of SO 3 H combined with water molecules through electrostatic interaction and hydrogen bonds to form the hydrated layers after the heparin immobilization. The hydration layers prevented the protein adsorption onto the membrane surfaces 32 . Compared with the pure PU membranes, PU/PDA‐Hep exhibited the lowest BSA and FBN adsorption of 2.51 ± 0.05 and 3.12 ± 0.12 μg/cm 2 ( p < 0.001), with a reduction of 38.99% and 35.83%, respectively.…”

“…The immobilization of heparin directly onto the surface of PU would seriously affect their biological activity 31 . Therefore, a crosslinking agent was needed to be introduced to covalently immobilize heparin onto the PU surface 32 . The existence of cross‐linking agents provided extra space and mobility for heparin.…”

Bioinspired Polycation Functionalization of the Polyurethane Surface for Enhanced Lubrication, Antibacterial Property, and Anticoagulation

“…The degree of protein adsorption on the PU samples was tested employing the method reported in the literature. 33 All samples were placed in a 24-well plate and immersed with PBS at 37 °C for 24 h. After removing PBS, 1 mL of bovine serum albumin (BSA) solution (0.5 mg mL −1 ; PBS was used as the solvent) was added to each well containing the samples and incubated at 37 °C for 2 h. Afterward, the samples were rinsed gently with PBS to remove the non-adsorbed proteins, and the adhered proteins on the samples were obtained by sonication using 2 wt % sodium dodecyl sulfonate at 37 °C for 30 min. Finally, the protein concentration was determined using the BCA Protein Assay Kit, and the amount of adsorbed protein was calculated according to the instructions of the manufacturer.…”

An anticoagulant/hemostatic indwelling needle for oral glucose tolerance test

“…Grafting bio-functional coatings on the surface of biomaterials to improve the hemocompatibility of devices has received extensive attention. 11–13 The surface immobilization of heparin has been frequently used to afford anticoagulant activity, while avoiding the side effects of free heparin. 12–15 However, the surface of FEP is inert and is difficult to modify, and the anticoagulation coating needs to be anchored on the inner surface of the indwelling needle, which limits the use of many surface treatment methods.…”

“…11–13 The surface immobilization of heparin has been frequently used to afford anticoagulant activity, while avoiding the side effects of free heparin. 12–15 However, the surface of FEP is inert and is difficult to modify, and the anticoagulation coating needs to be anchored on the inner surface of the indwelling needle, which limits the use of many surface treatment methods. In addition, in the structure of the indwelling needle, the catheter and the needle core are closely attached, thus restricting the thickness of the coating to micro- to nano-scale.…”