Hepatic biomarkers of xenobiotic metabolism in eighteen marine fish from NW Mediterranean shelf and slope waters in relation to some of their biological and ecological variables

Abstract

Please cite this article as: Solé, M., Antó, M., Baena, M., Carrasson, M., Cartes, J.E., Maynou, F. Hepatic biomarkers of xenobiotic metabolism in eighteen marine fish from NW Mediterranean shelf and slope waters in relation to some of their biological and ecological variables, Marine Environmental Research (2010), doi: 10.1016/j.marenvres.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. carboxylesterase and glutathione S-transferase. As markers at higher levels of biological organization, feeding preferences (on benthic, suprabenthic or zooplanktonic species), swimming capability, stomach fullness and trophic level were considered. Significant species differences were found among all the biochemical parameters analysed, although no relationships among the biomarkers themselves were evidenced. In general enzymatic activities were much higher in teleosts than in elasmobranchs, and in perciforms than in gadiforms. Seasonality was observed in some species with higher activities usually corresponding to the winter period. No site related differences were observed in the two selected sites, which differ over a small pollution gradient. A multivariate canonical correspondence analysis (CCA) was performed on shelf and slope species separately to relate biochemical markers with ecological variables. CCA revealed that for shelf species, EROD was positively related to benthos feeding as well as trophic level, while on the slope the clearest association was between suprabenthos feeders and trophic level. Our present results, including seasonality, slightly differ from former observations (Solé et al., 2009a) and reveal a more significant role of the ecological variables in controlling biomarkers expression in fish from the shelf.

Biomarkers and bioindicators of the environmental condition using a fish species (Pimelodus maculatus Lacepède, 1803) in a tropical reservoir in Southeastern Brazil

“…The recent advances in the ecotoxicology field had supplied a number of the biomarkers in organism, which can be used to estimate either exposure to chemicals or resultant effects (Handy et al, 2003;Lu et al, 2009;Ramsdorf et al, 2012). Biomarkers and bioindicators has been extensively used as proxies to determine responses at individual level to stressors, with biomarkers being more species-specific and with higher variability of responses compared to bioindicators (Schulz and MartinsJunior, 2001;Solé et al, 2010;Linde-Arias et al, 2008a, b;Barrilli et al, 2015). The integrated use of biomarkers and bioindicators is suggested as an evaluation tool, since they are effective means to determine the impact of pollution in the aquatic environment (Mc Carthy and Schugart, 1990;Reynolds et al, 2003).…”

“…Because the liver is so important in detoxification, exposure to contaminants can lead to an increase in liver size from hypertrophy (an increase in size), hyperplasia (an increase in number) of hepatocytes (Goede and Barton, 1990;Solé et al, 2010), or both. Studies evaluating the relative liver size of fishes from contaminated sites and last disturbed sites often utilize the HSI, which expresses liver size as a percentage of total body weight (Facey et al, 1999;Sadekarpawar and Parikh, 2013).…”

Metallothionein, oxidative stress and trace metals in gills and liver of demersal and pelagic fish species from Kuwaits’ marine area

“…Trace metals are known to exert oxidative stress on aquatic biota and generate reactive oxygen species (ROS) which otherwise are also produced by normal metabolism. Marine fish are known to have the ability to detoxify reactive oxygen species (Bigot et al, 2009;Sole et al, 2010). Metallothioneins are known to play an important role in quenching the effects of reactive oxygen species.…”

“…The other enzyme that catalyzes organic and inorganic peroxides is selenium dependent glutathione peroxidase (Se-GPx), the other isoform of this enzyme selenium independent GPx reduces only organic peroxides (Bigot et al, 2009). Glutathione reductase (GR) is another antioxidant enzyme that maintains the balance of reduced and oxidized glutathione (GSH/GSSG) in favor of its reduced form GSH which acts as a soluble antioxidant by itself and also plays a role as cofactor in other enzymatic activities (Sole et al, 2010). A gross biomarker of oxidative injury is a measure of thiobarbituric acid reactive substance (TBARS) like malondialdehyde (MDA) in tissues, indicating lipid peroxidation of the membranes (Parvez et al, 2006;Bergayou et al, 2009 Therefore, in this study metallothioneins, as scavenging protein for metals and biomarkers of oxidative stress were determined in the yellowfin seabream (Acanthopagrus latus), and tonguesole (Cynoglossus arel) collected from the two sampling stations one inside Kuwait Bay and a second the Auha area outside the Bay.…”

Natural variability of hepatic biomarkers in Mediterranean deep-sea organisms

“…This shrimp species is also one of the most valuable fishery resources in the Mediterranean (Company et al, 2008). All three species have been previously used in environmental monitoring studies conducted in Mediterranean deep-sea habitats (Escartin and Porte, 1999;Porte et al, 2000;Antó et al, 2009;Solé et al, 2009Solé et al, , 2010.…”