Hepatic Cellular Distribution of Silica Nanoparticles by Surface Energy Modification

Abstract

The cellular distribution of silica nanoparticles (NPs) in the liver is not well understood. Targeting specific cells is one of the most important issues in NP-based drug delivery to improve delivery efficacy. In this context, the present study analyzed the relative cellular distribution pattern of silica NPs in the liver, and the effect of surface energy modification on NPs. Hydrophobic NP surface modification enhanced NP delivery to the liver and liver sinusoid fFendothelial cells (LSECs). Conversely, hydrophilic NP surface modification was commensurate with targeting hepatic stellate cells (HSCs) rather than other cell types. There was no notable difference in NP delivery to Kupffer cells or hepatocytes, regardless of hydrophilic or hydrophobic NP surface modification, suggesting that both the targeting of hepatocytes and evasion of phagocytosis by Kupffer cells are not associated with surface energy modification of silica NPs. This study provides useful information to target specific cell types using silica NPs, as well as to understand the relationship between NP surface energy and the NP distribution pattern in the liver, thereby helping to establish strategies for cell targeting using various NPs.

Isolation and Characterization of Compounds from Glycyrrhiza uralensis as Therapeutic Agents for the Muscle Disorders

“…Muscle tissues were routinely fixed with 10% formalin and embedded in paraffin wax [ 60 ]. Paraffin-embedded tissue sections were deparaffinized, hydrated, and endogenous peroxidase was quenched using xylene (Junsei, Tokyo, Japan), an ethanol gradient, and methanol/H 2 O 2 (Junsei), respectively.…”

Different coatings on magnetic nanoparticles dictate their degradation kinetics in vivo for 15 months after intravenous administration in mice

“…In the figures that the area in question is not indicated, it is because it coincides with the bottom right inset area where the image is placed or the zoom corresponds to another of the images digestion [80], and they are thought to recognize MNPs as foreign material and internalize them through multiple receptors [37]. The uptake and retention of MNPs by KCs is strongly correlated with their surface charge, the nature of their chemical coating and their size [87]. Larger particles are usually phagocytosed more easily by this cell type, while MNPs with strongly cationic and anionic surface charges adsorb a quantity of serum proteins to form their PC and can aggregate, interacting more readily with macrophages in vitro.…”

Role of Liver-Mediated Tolerance in Nanoparticle-Based Tumor Therapy

“…The potential of LSECs as a target for immunotherapy has scarcely been issued yet. Finding a way to adjust the NP surface for LSEC targeting is a universal approach to improve the efficacy of NP targeting and drug delivery to endothelial cell types in the liver [ 4 ]. LSECs are specialized in the uptake of soluble material and of immune complexes which have a mean diameter of about 40 nm, at a range from 20–150 nm [ 21 ].…”

“…During the last decades, the development of nanoparticles (NPs) that deliver drugs and biologicals in a cell type-specific manner has received growing interest as a new therapeutic strategy in cancer therapy [ 1 ]. Targeting may be an intrinsic property of the NP due to its size and surface properties [ 2 ] or can be conferred by conjugated moieties that bind target cell surface receptors, including antibodies, derivatives of natural ligands, and aptamers [ 3 , 4 ].…”